Title:
METHOD FOR PREPARING ROSUVASTATIN INTERMEDIATE
Document Type and Number:
WIPO Patent Application WO/2014/205917
Kind Code:
A1
Abstract:
Provided is a method for preparing a rosuvastatin intermediate which is (R)-3-hydroxy-monomethyl glutarate and hydroxyl-protected (R)-3-hydroxy- monomethyl glutarate. (R)-4-cyano-3-hydroxy-methyl butyrate serving as a substrate is subjected to reaction under the action of an enzyme to generate the (R)-3-hydroxy- monomethyl glutarate, the enzyme is a recombinant nitrilase, and the reaction is carried out in an aqueous-phase buffering solution with the pH of 7.0 to 9.0 at the temperature of 25 to 35 degrees centigrade. The method has the characteristics of mild reaction conditions and high reaction efficiency, and is easy and convenient to operate; a specific recombinant nitrilase is used, thereby solving the problems of ultralow concentration of the substrate and low conversion rate in an existing biological method.
Inventors:
TAO JUNHUA (CN)
TANG SUSU (CN)
LI BIN (CN)
LIU GEN (CN)
TANG SUSU (CN)
LI BIN (CN)
LIU GEN (CN)
Application Number:
PCT/CN2013/083294
Publication Date:
December 31, 2014
Filing Date:
September 11, 2013
Export Citation:
Assignee:
ENZYMEWORKS INC (CN)
International Classes:
C12P7/62; C12N9/78; C12R1/19
Foreign References:
| CN101805756A | 2010-08-18 | |||
| CN101072871A | 2007-11-14 | |||
| CN102181493A | 2011-09-14 |
Attorney, Agent or Firm:
SUZHOU CREATOR PATENT & TRADEMARK AGENCY LTD. (CN)
苏州创元专利商标事务所有限公司 (CN)
苏州创元专利商标事务所有限公司 (CN)
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