METHOD FOR PREPARING SMALL-MOLECULE ASS-HIDE GLUE POLYPEPTIDE PRODUCT

WIPO Patent Application WO/2024/074159

A1

A method for preparing a small-molecule ass-hide glue polypeptide product. The method comprises the following steps: S1. pretreatment of ass-hide glue: crushing ass-hide glue blocks as raw materials, dissolving same by adding water, performing centrifugation to remove grease on the upper layer, and performing filtration to obtain an ass-hide glue solution; S2. enzymatic hydrolysis treatment of ass-hide glue: adjusting the pH of the ass-hide glue solution to 5.5, adding papain, and deactivating the enzyme after the enzymatic hydrolysis reaction is completed; and then adjusting the pH of the solution to 6.0, adding a compound bio-enzyme, and deactivating the enzyme after the enzymatic hydrolysis reaction is completed to obtain an ass-hide glue enzymatic hydrolyzate; S3. posttreatment of ass-hide glue: sequentially performing filtering, odor removing and drying crushing treatment on the ass-hide glue enzymatic hydrolyzate to obtain an ass-hide glue polypeptide compound for later use; S4. treatment of adjuvant: crushing an adjuvant, and then performing alcohol extraction to obtain an alcohol extract; performing water extraction on the remaining drug residues after the alcohol extraction of the adjuvant to obtain a water extract; and respectively distilling the alcohol extract and the water extract under reduced pressure to obtain an alcohol extract concentrate and a water extract concentrate, and then combining and drying the alcohol extract concentrate and the water extract concentrate to obtain a liquid extract of the adjuvant; and S5, preparation of a product: preparing the ass-hide glue polypeptide compound, the liquid extract of the adjuvant and a pharmaceutically acceptable carrier into a solid oral preparation, i.e., the small-molecule ass-hide glue polypeptide product. The above-mentioned preparation method has a high preparation efficiency and strong operability, and is easy to industrialize.

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WANG CHAO (CN)
ZHAO QIANG (CN)
SONG WENLU (CN)
AN XIAOYA (CN)
WANG YIHENG (CN)
HAN SHUYANG (CN)
ZHANG YINUO (CN)
ZHU MINGYUE (CN)
SHI RUIRUI (CN)
FENG LEI (CN)

PCT/CN2023/136381

April 11, 2024

December 05, 2023

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UNIV JINING (CN)

A23L33/18; A23L33/105; C07K1/34; C12P21/06

CN116649580A	2023-08-29
CN115724947A	2023-03-03
CN102101884A	2011-06-22
CN107998333A	2018-05-08
CN113332415A	2021-09-03
CN115088849A	2022-09-23

QINGDAO FIRST PATENT & TRADEMARK AGENT CO., LTD. (CN)

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