Title:
METHOD FOR PREPARING TRANSGENIC ANIMAL
Document Type and Number:
WIPO Patent Application WO/1997/011597
Kind Code:
A1
Abstract:
A method for preparing spermatozoa having an alien DNA of a mature male nonhuman vertebrate transduced thereinto which comprises injecting the alien DNA carried by a liposome for transduction into the testes of the animal thrice or more repeatedly and thus producing spermatozoa having the alien DNA transduced thereinto in the testes of the animal. A transgenic nonhuman vertebrate can be conveniently and efficiently produced by fertilizing an unfertilized egg with such a spermatozoon by natural crossing, artificial fertilization or external fertilization.
Inventors:
OGAWA SHYOSO (JP)
IWAYA MAKOTO (JP)
SATOH MASAHIRO (JP)
TADA NORIHIRO (JP)
IWAYA MAKOTO (JP)
SATOH MASAHIRO (JP)
TADA NORIHIRO (JP)
Application Number:
PCT/JP1996/002828
Publication Date:
April 03, 1997
Filing Date:
September 27, 1996
Export Citation:
Assignee:
HOECHST JAPAN (JP)
OGAWA SHYOSO (JP)
IWAYA MAKOTO (JP)
SATOH MASAHIRO (JP)
TADA NORIHIRO (JP)
OGAWA SHYOSO (JP)
IWAYA MAKOTO (JP)
SATOH MASAHIRO (JP)
TADA NORIHIRO (JP)
International Classes:
A01K67/027; C12N5/10; C12N15/09; C12N15/87; C12N15/88; (IPC1-7): A01K67/027
Other References:
ANIMAL BIOTECH., Vol. 5, No. 1, (1994), M. SATO et al., "Direct Injection Offoreign DNA Into Mouse Testis as a Possible Alternative of Sperm-Mediated Gene Transfer", p. 19-31.
SCIENCE, Vol. 261, (1993), ZHU, N. et al., "Systemic Gene Expression After Intravenous DNA Delivery Into Adult Mice", p. 209-211.
MOL. REPROD. DEV., Vol. 30, (1993), BACHILLER, D. et al., "Liposome-Mediated DNA Uptake by Sperm Cells", p. 194-200.
PROC. NATL. ACAD. SCI. U.S.A., Vol. 86, (1989), J.P. BEHR et al., "Efficient Gene Transfer Into Mammalian Primary Endocrine Cells With Lipopolyamine-Coated DNA", p. 6982-6986.
See also references of EP 0867114A4
SCIENCE, Vol. 261, (1993), ZHU, N. et al., "Systemic Gene Expression After Intravenous DNA Delivery Into Adult Mice", p. 209-211.
MOL. REPROD. DEV., Vol. 30, (1993), BACHILLER, D. et al., "Liposome-Mediated DNA Uptake by Sperm Cells", p. 194-200.
PROC. NATL. ACAD. SCI. U.S.A., Vol. 86, (1989), J.P. BEHR et al., "Efficient Gene Transfer Into Mammalian Primary Endocrine Cells With Lipopolyamine-Coated DNA", p. 6982-6986.
See also references of EP 0867114A4
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