Title:
METHOD FOR PRETREATING BIOLOGICAL PARTICLES-CONTAINING SAMPLE, METHOD FOR ACQUIRING IMAGE OF BIOLOGICAL PARTICLES, DEVICE FOR PRETREATING BIOLOGICAL PARTICLES-CONTAINING SAMPLE, AND DEVICE FOR ACQUIRING IMAGE OF BIOLOGICAL PARTICLES
Document Type and Number:
WIPO Patent Application WO/2018/101194
Kind Code:
A1
Abstract:
The present invention relates to a method for pretreating a sample including biological particles, the method comprising: a step of acquiring a fraction (1b), which passes through a sieve (A) having a sieve mesh size of 250-1000 μm and does not pass through a sieve (B) having a sieve mesh size of 32-63 μm, by sieving a biological particles-containing sample to be detected; and a step of adding a colloidal solution having a density of 1.10-2.45 g/cm3 to the fraction (1b), centrifuging the resultant solution, and acquiring a supernatant fraction (S0) after centrifugation.
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Inventors:
KITAHASHI TOMO (JP)
WATANABE HIROMI (JP)
TSUCHIYA MASASHI (JP)
YAMAMOTO HIROYUKI (JP)
WATANABE HIROMI (JP)
TSUCHIYA MASASHI (JP)
YAMAMOTO HIROYUKI (JP)
Application Number:
PCT/JP2017/042355
Publication Date:
June 07, 2018
Filing Date:
November 27, 2017
Export Citation:
Assignee:
JAPAN AGENCY MARINE EARTH SCI (JP)
International Classes:
C12N1/02; C12M1/00; G01N1/10; G01N15/00
Foreign References:
| JP2016232868A | 2016-11-30 | |||
| US20140146157A1 | 2014-05-29 |
Other References:
GREGO M. ET AL.: "Main meiofauna taxa as an indicator for assessing the spatial and seasonal impact of fish farming", MAR. POLLUT. BULL., vol. 58, no. 8, August 2009 (2009-08-01), pages 1178 - 1186, XP085618438
BURGESS R. ET AL.: "An improved protocol for separating meiofauna from sediments using colloidal silica sols", MARINE ECOLOGY PROGRESS SERIES, vol. 214, 2001, pages 161 - 165, XP055567954
CARSTENSEN J. ET AL.: "7. 08-coastal monitoring programs", TREATISE ON ESTUARINE AND COASTAL SCIENCE, vol. 7, 2011, pages 175 - 206, XP055528357, DOI: doi:10.1016/B978-0-12-374711-2.00712-9
See also references of EP 3480294A4
BURGESS R. ET AL.: "An improved protocol for separating meiofauna from sediments using colloidal silica sols", MARINE ECOLOGY PROGRESS SERIES, vol. 214, 2001, pages 161 - 165, XP055567954
CARSTENSEN J. ET AL.: "7. 08-coastal monitoring programs", TREATISE ON ESTUARINE AND COASTAL SCIENCE, vol. 7, 2011, pages 175 - 206, XP055528357, DOI: doi:10.1016/B978-0-12-374711-2.00712-9
See also references of EP 3480294A4
Attorney, Agent or Firm:
TANAI Sumio et al. (JP)
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