Title:
METHOD FOR PRETREATING LIGNOCELLULOSIC BIOMASS MATCHED WITH SACCHARIFICATION OF WHOLE FIBROSOME
Document Type and Number:
WIPO Patent Application WO/2020/034395
Kind Code:
A1
Abstract:
On the basis of existing problems in the prior art in the pretreatment of lignocellulose, the present invention provides a method suitable for pretreating lignocellulosic biomass, suitable for producing fermentable sugars by saccharifying cellulose substrates of cellulosome whole bacteria. The pretreatment method comprises three steps performed in sequence: (1) physical pulverization, (2) sulfonation pretreatment, and (3) hydrothermal pretreatment/xylanase hydrolysis pretreatment. The (2) sulfonation pretreatment step must be set before the (3) hydrothermal pretreatment/xylanase hydrolysis pretreatment step. The method achieves green and efficient separation and comprehensive utilization of three major components of biomass: cellulose, hemicellulose, and lignin. Furthermore, the cellulose substrate obtained by the method is used for bacterial saccharification of fibrosomes, hydrolytic saccharification efficiency is greatly improved, producing an unexpected technical effect, and has significant progress.
Inventors:
LI BIN (CN)
YU GUANG (CN)
LIU YAJUN (CN)
LIU CHAO (CN)
CUI QIU (CN)
ZHANG YUEDONG (CN)
PENG HUI (CN)
YU GUANG (CN)
LIU YAJUN (CN)
LIU CHAO (CN)
CUI QIU (CN)
ZHANG YUEDONG (CN)
PENG HUI (CN)
Application Number:
PCT/CN2018/112317
Publication Date:
February 20, 2020
Filing Date:
October 29, 2018
Export Citation:
Assignee:
QINGDAO INST BIOENERGY & BIOPROCESS TECH CAS (CN)
International Classes:
C12P7/10; C12P19/02; C12P19/04; D21C1/02; D21C3/06
Domestic Patent References:
| WO2013034818A1 | 2013-03-14 |
Foreign References:
| CN105625075A | 2016-06-01 | |||
| CN106191158A | 2016-12-07 | |||
| CN102174594A | 2011-09-07 | |||
| EP2804951A1 | 2014-11-26 |
Other References:
LI, QIANG ET AL.: "Improving Alkaline Pretreatment of Corn Stover to Enhance Enzymatic Saccharification", BIOREFINERY, vol. 33, no. 10, 31 May 2012 (2012-05-31), pages 51 - 55
Attorney, Agent or Firm:
QINGDAO HECHUANG INTELLECTUAL PROPERTY AGENCY FIRM (GENERAL PARTNERSHIP ) (CN)
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