Title:
METHOD FOR PROCESSING BIOLOGICAL SAMPLES
Document Type and Number:
WIPO Patent Application WO2001020042
Kind Code:
A3
Abstract:
Methods, compositions and systems for processing biological samples employ microparticulate separation reagents to capture a target species from a biological sample. Photoactivation of a photoaffinity label coupled to the separation reagent creates a covalent adduct of the separation reagent and target species. The adduct is captured and DNA from the target species is analyzed using known techniques. Capture is accomplished using magnetic beads, filtration, centrifugation or other separation techniques. The method of the invention is amenable to complete automation.
Inventors:
CHAPMAN WILLIAM H JR (US)
KLEVAN LEONARD (US)
KLEVAN LEONARD (US)
Application Number:
PCT/US2000/025423
Publication Date:
November 22, 2001
Filing Date:
September 15, 2000
Export Citation:
Assignee:
MIRAIBIO INC (US)
CHAPMAN WILLIAM H JR (US)
KLEVAN LEONARD (US)
CHAPMAN WILLIAM H JR (US)
KLEVAN LEONARD (US)
International Classes:
G01N33/53; C12M1/00; C12Q1/68; C12Q1/6804; C12Q1/6834; G01N1/10; G01N33/48; G01N33/543; (IPC1-7): C12Q1/68; G01N33/50
Domestic Patent References:
| WO1995016204A1 | 1995-06-15 | |||
| WO1996031781A1 | 1996-10-10 | |||
| WO1994001773A1 | 1994-01-20 | |||
| WO1995031429A1 | 1995-11-23 |
Foreign References:
| US4716122A | 1987-12-29 | |||
| EP0475784A1 | 1992-03-18 | |||
| EP0408078A2 | 1991-01-16 | |||
| EP0156537A2 | 1985-10-02 | |||
| EP0332022A2 | 1989-09-13 | |||
| US4879220A | 1989-11-07 |
Other References:
SAFARK I ET AL: "Use of magnetic techniques for the isolation of cells", JOURNAL OF CHROMATOGRAPHY B: BIOMEDICAL APPLICATIONS,NL,ELSEVIER SCIENCE PUBLISHERS, vol. 722, no. 1-2, 5 February 1999 (1999-02-05), pages 33 - 53, XP004156204, ISSN: 0378-4347
MARGEL SHLOMO ET AL: "Synthesis, characterization, and use of new solid and hollow, magnetic and non-magnetic, organic-inorganic monodispersed hybrid microspheres.", SCIENTIFIC AND CLINICAL APPLICATIONS OF MAGNETIC CARRIERS., 1997, International Conference;Rostock, Germany; September 5-7, 1996, Plenum Press;Plenum Press 233 Spring Street, New York, New York, USA; London, England, UK, pages 37 - 51, XP001009773, ISBN: 0-306-45687-7
MARGEL ET AL.: "Functional nanospheres: synthesis and biological applications", RECENT RES.DEVEL.IN POLYMER SCIENCE, vol. 1, 1997, pages 51 - 78, XP001009817
KAUSCH ALBERT P ET AL: "Organelle isolation by magnetic immunoabsorption.", BIOTECHNIQUES, vol. 26, no. 2, February 1999 (1999-02-01), pages 336 - 343, XP002170373, ISSN: 0736-6205
EBERLE A N: "PHOTO AFFINITY LABELING OF PEPTIDE HORMONE RECEPTORS", JOURNAL OF RECEPTOR RESEARCH, vol. 3, no. 1-2, 1983, pages 313 - 326, XP001009839, ISSN: 0197-5110
MARGEL SHLOMO ET AL: "Synthesis, characterization, and use of new solid and hollow, magnetic and non-magnetic, organic-inorganic monodispersed hybrid microspheres.", SCIENTIFIC AND CLINICAL APPLICATIONS OF MAGNETIC CARRIERS., 1997, International Conference;Rostock, Germany; September 5-7, 1996, Plenum Press;Plenum Press 233 Spring Street, New York, New York, USA; London, England, UK, pages 37 - 51, XP001009773, ISBN: 0-306-45687-7
MARGEL ET AL.: "Functional nanospheres: synthesis and biological applications", RECENT RES.DEVEL.IN POLYMER SCIENCE, vol. 1, 1997, pages 51 - 78, XP001009817
KAUSCH ALBERT P ET AL: "Organelle isolation by magnetic immunoabsorption.", BIOTECHNIQUES, vol. 26, no. 2, February 1999 (1999-02-01), pages 336 - 343, XP002170373, ISSN: 0736-6205
EBERLE A N: "PHOTO AFFINITY LABELING OF PEPTIDE HORMONE RECEPTORS", JOURNAL OF RECEPTOR RESEARCH, vol. 3, no. 1-2, 1983, pages 313 - 326, XP001009839, ISSN: 0197-5110
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