Title:
METHOD FOR PRODUCING 1,3-PROPYLENE GLYCOL BY MEANS OF FERMENTATION OF RECOMBINANT MICROORGANISM
Document Type and Number:
WIPO Patent Application WO/2019/119789
Kind Code:
A1
Abstract:
Provided is a method for producing 1,3-propylene glycol by means of fermentation of a recombinant microorganism. First, a recombinant microorganism is provided; the recombinant microorganism can overexpress the acetyl-CoA carboxylase gene accBC and accDA, express the malonyl-CoA synthetase gene mcr, and express the 3-hydroxypropionyl-CoA synthetase gene pcs, the 3-hydroxypropionyl-CoA reductase gene pduP and the 1,3-propylene glycol reductase gene yqhD. The recombinant microorganism is subjected to fermentation culture in a shake flask or a fermentor using glucose as a substrate to obtain 1,3-propylene glycol. The recombinant microorganism can utilize low-cost glucose, sucrose, molasses, xylose and the like as raw materials in the fermentation process, and expensive coenzyme B12 is not needed, thus, production cost is significantly reduced, and a good market application prospect is brought about.
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Inventors:
CHEN ZHEN (CN)
LIU DEHUA (CN)
LIU DEHUA (CN)
Application Number:
PCT/CN2018/095893
Publication Date:
June 27, 2019
Filing Date:
July 17, 2018
Export Citation:
Assignee:
UNIV TSINGHUA (CN)
International Classes:
C12N1/21; C12P7/18
Foreign References:
| CN108085288A | 2018-05-29 | |||
| CN107400652A | 2017-11-28 | |||
| CN106906248A | 2017-06-30 | |||
| CN200380104657A | 2003-10-06 |
Other References:
FENG, XINJUN ET AL.: "Research and Development of 3-Hydroxypropionic Acid by Microbes and Engineered Cells", BIOTECHNOLOGY & BUSINESS, 30 November 2017 (2017-11-30), pages 30 - 43, XP009521789, ISSN: 1674-0319
LEE, M.S. ET AL.: "Enhancement of 1,3-propanediol Production by Expression of Pyruvate Decarboxylase and Aldehyde Dehydrogenase from Zymomonas Mobilis in the Acetolactate- synthase-deficient Mutant of Klebsiella Pneumoniae", JOURNAL OF INDUSTRIAL MICROBIOLOGY AND BIOTECHNOLOGY, vol. 41, no. 8, 20 May 2014 (2014-05-20), pages 1259 - 1266, XP055619622, DOI: 10.1007/s10295-014-1456-x
LI HONGMEI ET AL.: "Cloning and Expression of 1,3-Propenediol Oxidoreductase Isoenzyme Gene yqhd from Escherichia coli", CHEMICAL INDUSTRY AND ENGINEERING PROGRESS, vol. 27, no. 4, 31 December 2008 (2008-12-31), pages 527 - 530, XP009521779
See also references of EP 3730602A4
LEE, M.S. ET AL.: "Enhancement of 1,3-propanediol Production by Expression of Pyruvate Decarboxylase and Aldehyde Dehydrogenase from Zymomonas Mobilis in the Acetolactate- synthase-deficient Mutant of Klebsiella Pneumoniae", JOURNAL OF INDUSTRIAL MICROBIOLOGY AND BIOTECHNOLOGY, vol. 41, no. 8, 20 May 2014 (2014-05-20), pages 1259 - 1266, XP055619622, DOI: 10.1007/s10295-014-1456-x
LI HONGMEI ET AL.: "Cloning and Expression of 1,3-Propenediol Oxidoreductase Isoenzyme Gene yqhd from Escherichia coli", CHEMICAL INDUSTRY AND ENGINEERING PROGRESS, vol. 27, no. 4, 31 December 2008 (2008-12-31), pages 527 - 530, XP009521779
See also references of EP 3730602A4
Attorney, Agent or Firm:
NTD UNIVATION INTELLECTUAL PROPERTY AGENCY LTD. (CN)
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