Title:
METHOD OF PRODUCING 6-AMINOPENICILLANIC ACID USING THE ACULEACIN A ACYLASE ENZYME OF ACTINOPLANES UTAHENSIS, WHICH IS PURIFIED FROM RECOMBINANT BACTERIA STREPTOMYCES LIVIDANS CECT 3377
Document Type and Number:
WIPO Patent Application WO2006058942
Kind Code:
A3
Abstract:
The invention relates to a method of producing 6-aminopenicillanic acid using the aculeacin A acylase enzyme of Actinoplanes utahensis, which is purified from recombinant bacteria Streptomyces lividans CECT 3377. More specifically, the invention relates to a method of producing 6-aminopenicillanic acid, comprising the hydrolysis of penicillins V, K, F or dihydroF, using the aculeacin A acylase enzyme of bacteria Actinoplanes utahensis which is purified from broth cultures of cells of recombinant bacteria Streptomyces lividans CECT 3367 which carry the aac gene that encodes said enzyme.
Inventors:
DE LA MATA RIESCO ISABEL (ES)
TORRES BACETE JESUS (ES)
HORMIGO CISNEROS DANIEL (ES)
STUART LEON MARIBEL (ES)
ARROYO SANCHEZ MIGUEL (ES)
TORRES BACETE JESUS (ES)
HORMIGO CISNEROS DANIEL (ES)
STUART LEON MARIBEL (ES)
ARROYO SANCHEZ MIGUEL (ES)
Application Number:
PCT/ES2005/000643
Publication Date:
August 24, 2006
Filing Date:
November 25, 2005
Export Citation:
Assignee:
UNIV COMPLUTENSE DE MADRID REC (ES)
DE LA MATA RIESCO ISABEL (ES)
TORRES BACETE JESUS (ES)
HORMIGO CISNEROS DANIEL (ES)
STUART LEON MARIBEL (ES)
ARROYO SANCHEZ MIGUEL (ES)
DE LA MATA RIESCO ISABEL (ES)
TORRES BACETE JESUS (ES)
HORMIGO CISNEROS DANIEL (ES)
STUART LEON MARIBEL (ES)
ARROYO SANCHEZ MIGUEL (ES)
International Classes:
C07D499/44; C12P37/06
Foreign References:
| ES2105989A1 | 1997-10-16 |
Other References:
PARMAR A. ET AL.: "Advances in enzymatic transformation of penicillins to 6-aminopenicillanic acid (6-APA)", BIOTECHNOLOGY ADVANCES, vol. 18, 2000, pages 289 - 301, XP004213782
INOKOSHI J. ET AL.: "Efficient production of aculeacin-A acylase in recombinant streptomyces strains", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 39, no. 4-5, July 1993 (1993-07-01), pages 532 - 536, XP001095451
INOKOSHI J. ET AL.: "Identification of precursor peptide of aculeacin A acylase as a protein with proteolytic activity", FEMS MICROBIOLOGY LETTERS, vol. 114, no. 3, 15 December 1993 (1993-12-15), pages 305 - 309
KREUZMAN A.J. ET AL.: "Membrane-associated echinocndin B deacylase of Actinoplanes utahensis: purification, characterization, heterologous cloning and enzymatic deacylation reaction", JOURNAL OF INDUSTRIAL MICROBIOLOGY AND BIOTECHNOLOGY, vol. 24, no. 3, March 2000 (2000-03-01), pages 173 - 180
INOKOSHI J. ET AL.: "Efficient production of aculeacin-A acylase in recombinant streptomyces strains", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 39, no. 4-5, July 1993 (1993-07-01), pages 532 - 536, XP001095451
INOKOSHI J. ET AL.: "Identification of precursor peptide of aculeacin A acylase as a protein with proteolytic activity", FEMS MICROBIOLOGY LETTERS, vol. 114, no. 3, 15 December 1993 (1993-12-15), pages 305 - 309
KREUZMAN A.J. ET AL.: "Membrane-associated echinocndin B deacylase of Actinoplanes utahensis: purification, characterization, heterologous cloning and enzymatic deacylation reaction", JOURNAL OF INDUSTRIAL MICROBIOLOGY AND BIOTECHNOLOGY, vol. 24, no. 3, March 2000 (2000-03-01), pages 173 - 180
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