Title:
METHOD FOR PRODUCING AFFINITY SEPARATION MATRIX, AND AFFINITY SEPARATION MATRIX
Document Type and Number:
WIPO Patent Application WO/2017/195641
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide an affinity separation matrix having extremely excellent binding capacity and binding efficiency with respect to a κ-chain variable region-containing peptide, a method for producing the affinity separation matrix, and a method for producing a κ-chain variable region-containing peptide using the affinity separation matrix. The affinity separation matrix production method according to the present invention is characterized by including a step for immobilizing, as a ligand, a κ-chain variable region-containing peptide having a cysteine residue at the N-terminus and/or the C-terminus thereof to a water-insoluble carrier with the terminal cysteine residue being interposed therebetween.
Inventors:
MURATA DAI (JP)
YOSHIDA SHINICHI (JP)
YOSHIDA SHINICHI (JP)
Application Number:
PCT/JP2017/016819
Publication Date:
November 16, 2017
Filing Date:
April 27, 2017
Export Citation:
Assignee:
KANEKA CORP (JP)
International Classes:
C07K1/22; B01J20/24; B01J20/281; B01J20/30; C07K17/00; C07K16/00
Domestic Patent References:
| WO2016031902A1 | 2016-03-03 | |||
| WO2008044692A1 | 2008-04-17 |
Foreign References:
| JP2008523140A | 2008-07-03 |
Other References:
GRAILLE MARC ET AL.: "Complex between peptostreptococcus magnus Protein L and a Human antibody reveals structural convergence in the interaction modes of Fab binding proteins", STRUCTURE, vol. 9, no. 8, 2001, pages 679 - 687, XP055259618
Attorney, Agent or Firm:
UEKI, Kyuichi et al. (JP)
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