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Title:
METHOD FOR PRODUCING AGGREGATED AND IMMOBILIZED CELLS USING AQUEOUS PLASMARIZED PROTEIN SOLUTION, AND AGGREGATED AND IMMOBILIZED CELLS PRODUCED BY SAID PRODUCTION METHOD
Document Type and Number:
WIPO Patent Application WO/2016/143669
Kind Code:
A1
Abstract:
There is found a means for adhering cells or cell masses to each other with a protein such as an extracellular matrix protein to produce aggregated and immobilized cells. The purpose of the present invention is to apply the means for various purposes. Specifically, it is found that aggregated and immobilized cells can be produced by using an aqueous plasmarized protein solution in the form of mists or liquid droplets as an adhesion solution for cells or cell masses, wherein the aqueous plasmarized protein solution is produced by carrying out the plasmarization of an extracellular matrix protein by means of low-temperature plasma discharge preferably using a surface discharge-induced low-temperature plasma discharge device. This finding leads to the accomplishment of the present invention.

Inventors:
SATO MASAAKI (JP)
YOSHINO DAISUKE (JP)
Application Number:
PCT/JP2016/056632
Publication Date:
September 15, 2016
Filing Date:
March 03, 2016
Export Citation:
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Assignee:
UNIV TOHOKU (JP)
International Classes:
C12N5/07; C12N1/00; C12N5/071; C07K1/24; C07K14/78
Domestic Patent References:
WO2012118099A12012-09-07
WO2006093151A12006-09-08
WO2015079759A12015-06-04
Foreign References:
JP2003210156A2003-07-29
Attorney, Agent or Firm:
SHIMURA MITSUHARU (JP)
Mitsuharu Shimura (JP)
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