Title:
METHOD FOR PRODUCING ANTIBODY-LYSOSOMAL ENZYME FUSION PROTEIN
Document Type and Number:
WIPO Patent Application WO/2022/191158
Kind Code:
A1
Abstract:
Disclosed is a method for producing a fusion protein in which an antibody is fused with a lysosomal enzyme. This method for producing a fusion protein in which an antibody is fused with a human lysosomal enzyme comprises: (a) culturing mammalian cells that produce the fusion protein in serum-free culture medium and bringing about secretion of the fusion protein into the culture medium, (b) recovering the culture supernatant by removing the mammalian cells from the culture medium, and (c) purifying the fusion protein from the culture supernatant using: column chromatography that uses as the stationary phase a material to which a substance exhibiting affinity for the antibody is bonded, anion-exchange column chromatography, cation-exchange column chromatography, and size-exclusion column chromatography.
Inventors:
KAKIMOTO SHINJI (JP)
FUKUI TSUYOSHI (JP)
HATANO YUKICHI (JP)
KOTANI AYAKA (JP)
MIURA TAKUYA (JP)
ISHIGURO TOSHIFUMI (JP)
FUKUI TSUYOSHI (JP)
HATANO YUKICHI (JP)
KOTANI AYAKA (JP)
MIURA TAKUYA (JP)
ISHIGURO TOSHIFUMI (JP)
Application Number:
PCT/JP2022/009851
Publication Date:
September 15, 2022
Filing Date:
March 08, 2022
Export Citation:
Assignee:
JAPAN CHEM RES (JP)
International Classes:
C12P21/02; C07K1/16; C07K16/28; C07K19/00; C12N9/24; C12N15/13; C12N15/56; C12N15/62
Domestic Patent References:
| WO2018124121A1 | 2018-07-05 | |||
| WO2014017088A1 | 2014-01-30 | |||
| WO2016117341A1 | 2016-07-28 | |||
| WO2016067944A1 | 2016-05-06 | |||
| WO2018038243A1 | 2018-03-01 | |||
| WO2012063799A1 | 2012-05-18 | |||
| WO2013161958A1 | 2013-10-31 |
Foreign References:
| JP2021506875A | 2021-02-22 | |||
| JP2017132757A | 2017-08-03 | |||
| JP2014534055A | 2014-12-18 | |||
| JP2019521986A | 2019-08-08 | |||
| JP2010511378A | 2010-04-15 | |||
| JP2009273427A | 2009-11-26 | |||
| JP2014508506A | 2014-04-10 | |||
| JP6279466B2 | 2018-02-14 |
Other References:
ALTSCHUL SF., J MOL. BIOL., vol. 215, 1990, pages 403 - 10
PEARSONLIPMAN, PROC. NATL. ACAD. SCI. USA., vol. 85, 1988, pages 2444
SMITHWATERMAN, ADV. APPL. MATH., vol. 2, 1981, pages 482 - 9
MIYAHARA M., J. BIOL. CHEM., vol. 275, 2000, pages 613 - 618
PEARSONLIPMAN, PROC. NATL. ACAD. SCI. USA., vol. 85, 1988, pages 2444
SMITHWATERMAN, ADV. APPL. MATH., vol. 2, 1981, pages 482 - 9
MIYAHARA M., J. BIOL. CHEM., vol. 275, 2000, pages 613 - 618
Attorney, Agent or Firm:
YAMAMOTO, Yoshiki et al. (JP)
Download PDF: