Title:
METHOD OF PRODUCING BIOSPECIFIC MOLECULES BY PROTEIN TRANS-SPLICING
Document Type and Number:
WIPO Patent Application WO2002046208
Kind Code:
A3
Abstract:
The invention provides methods of using protein trans-splicing for the production of bispecific molecule which has a first antigen recognition portion that binds a C3b-like receptor and a second antigen recognition portion that binds an antigenic molecule present in the circulatory system of a mammal. The invention also provides bispecific molecules produced by protein trans-splicing. The bispecific molecules of the invention can be used for the clearance of pathogenic antigenic molecules from the circulatory system of a mammal. The invention further provides methods of using protein trans-splicing for the production of poylclonal libraries of bispecific molecules, which comprise populations of bispecific molecules with different antigen recognition specificities. Such polyclonal libraries of bispecific molecules can be used for targeting multiple epitopes of a pathogenic antigenic molecule and/or multiple variants of a pathogenic antigenic molecule.
Inventors:
HIMAWAN JEFF (US)
Application Number:
PCT/US2001/045653
Publication Date:
August 08, 2002
Filing Date:
November 01, 2001
Export Citation:
Assignee:
ELUSYS THERAPEUTICS INC (US)
HIMAWAN JEFF (US)
HIMAWAN JEFF (US)
International Classes:
A61K39/395; A61P31/04; A61P31/10; A61P31/12; C12N15/09; A61P31/18; A61P33/00; A61P33/02; A61P37/06; A61P39/02; C07K14/35; C07K14/735; C07K16/28; C07K16/46; C07K19/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/62; C12P21/02; C12P21/08; (IPC1-7): A61K39/395; A61K48/00; C12P21/06; C12N9/00
Foreign References:
| US6054312A | 2000-04-25 |
Other References:
SCOTT, P.C. ET AL.: "Production of cyclic peptides and proteins in vivo", PROC. NATL. ACAD. SCI. USA, vol. 96, 1999, pages 13638 - 13643, XP002137479
PLUECKTHUN ET AL.: "New protein engineering approaches to multivalent and bispecific antibody fragment", vol. 3, 1997, pages 83 - 105, XP002950976
NOREN, C.J. ET AL.: "Dissecting the chemistry of protein splicing and its applications", ANGEW. CHEM. INT. ED., vol. 39, 2000, pages 451 - 466, XP002950975
MCGUINNESS, B.T.J. ET AL.: "Phage diabody repertoires for selection of large numbers of bispecific antibody fragments", NATURE GENETICS, vol. 14, 1996, pages 1149 - 1154, XP002100039
PERLER, F.B.: "A natural example of protein trans-splicing", TRENDS BIOCHEM. SCI., vol. 24, 1999, pages 209 - 211, XP004170188
PLUECKTHUN ET AL.: "New protein engineering approaches to multivalent and bispecific antibody fragment", vol. 3, 1997, pages 83 - 105, XP002950976
NOREN, C.J. ET AL.: "Dissecting the chemistry of protein splicing and its applications", ANGEW. CHEM. INT. ED., vol. 39, 2000, pages 451 - 466, XP002950975
MCGUINNESS, B.T.J. ET AL.: "Phage diabody repertoires for selection of large numbers of bispecific antibody fragments", NATURE GENETICS, vol. 14, 1996, pages 1149 - 1154, XP002100039
PERLER, F.B.: "A natural example of protein trans-splicing", TRENDS BIOCHEM. SCI., vol. 24, 1999, pages 209 - 211, XP004170188
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