Title:
METHOD FOR PRODUCING BLOOD CHIMERIC ANIMAL
Document Type and Number:
WIPO Patent Application WO/2016/208532
Kind Code:
A1
Abstract:
Disclosed is a novel means capable of producing a blood chimeric animal that maintains, for a long period of time, a state in which blood cells derived from a heterologous animal such as a human are retained at a high rate even in medium- to large-sized livestock. The method for preparing a non-human animal that retains blood cells derived from a heterologous animal, according to the present invention, includes transplanting, into the non-human animal, hematopoietic cells of the heterologous animal in which the function of a gene acting on a hematopoietic system has been modified. The gene acting on a hematopoietic system is, for example, the Lnk gene. According to the method of the present invention, when a medium- to large-sized mammal such as a pig is used as a recipient, the survival rate of hematopoietic cells derived from a heterologous animal is dramatically increased such that blood chimerism of 10% or more can be maintained even in a 16 month old animal.
Inventors:
FUKAWA KAZUO (JP)
ITO TETSUYA (JP)
KAMIKAWA MAI (JP)
SENDAI YUTAKA (JP)
NAKAUCHI HIROMITSU (JP)
YAMAZAKI SATOSHI (JP)
WATANABE MOTOO (JP)
ITO TETSUYA (JP)
KAMIKAWA MAI (JP)
SENDAI YUTAKA (JP)
NAKAUCHI HIROMITSU (JP)
YAMAZAKI SATOSHI (JP)
WATANABE MOTOO (JP)
Application Number:
PCT/JP2016/068233
Publication Date:
December 29, 2016
Filing Date:
June 20, 2016
Export Citation:
Assignee:
NAT FEDERATION OF AGRICULTURAL COOP ASS (JP)
UNIV TOKYO (JP)
UNIV TOKYO (JP)
International Classes:
A01K67/027; C12N5/16; C12N15/09
Domestic Patent References:
| WO2011071085A1 | 2011-06-16 | |||
| WO2005019441A1 | 2005-03-03 |
Foreign References:
| JP2005229802A | 2005-09-02 |
Other References:
HITOSHI TAKIZAWA ET AL.: "Yokuseisei Adaptor Protein Lnk no Ikkasei Sogai ni yoru Zoketsu Kansaibo Oyobi Zenku Saibo no Kotsuzui Saikenno Seigyo", PROCEEDINGS OF THE JAPANESE SOCIETY FOR IMMUNOLOGY, vol. 34, 2004, pages 66, XP009507919, ISSN: 0919-1984
TAKIZAWA, H. ET AL.: "Enhanced engraftment of hematopoietic stem/progenitor cells by the transient inhibition of an adaptor protein, Lnk", BLOOD, vol. 107, no. 7, 2006, pages 2968 - 2975, XP055341166, ISSN: 0006-4971
SUZUKI, N. ET AL.: "Homeostasis of hematopoietic stem cells regulated by the myeloproliferative disease associated- gene product Lnk/Sh2b3 via Bcl-xL", EXPERIMENTAL HEMATOLOGY, vol. 40, 2012, pages 166 - 174, XP028437326, ISSN: 0301-472X
See also references of EP 3311660A4
TAKIZAWA, H. ET AL.: "Enhanced engraftment of hematopoietic stem/progenitor cells by the transient inhibition of an adaptor protein, Lnk", BLOOD, vol. 107, no. 7, 2006, pages 2968 - 2975, XP055341166, ISSN: 0006-4971
SUZUKI, N. ET AL.: "Homeostasis of hematopoietic stem cells regulated by the myeloproliferative disease associated- gene product Lnk/Sh2b3 via Bcl-xL", EXPERIMENTAL HEMATOLOGY, vol. 40, 2012, pages 166 - 174, XP028437326, ISSN: 0301-472X
See also references of EP 3311660A4
Attorney, Agent or Firm:
TANIGAWA AND PARTNERS, PATENT FIRM (JP)
Patent business corporation mountain stream international patent firm (JP)
Patent business corporation mountain stream international patent firm (JP)
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