Title:
METHOD FOR PRODUCING BRAIN ORGANOIDS
Document Type and Number:
WIPO Patent Application WO/2020/100481
Kind Code:
A1
Abstract:
A method for producing brain organoids comprising a step of culturing a neuroectoderm marker-positive cell aggregate in a culture medium that contains extracellular matrix at a concentration of more than 10 volume%.
Inventors:
HIRAMINE HAYATO (JP)
ISHIKAWA MITSURU (JP)
OKANO HIDEYUKI (JP)
ISHIKAWA MITSURU (JP)
OKANO HIDEYUKI (JP)
Application Number:
PCT/JP2019/040001
Publication Date:
May 22, 2020
Filing Date:
October 10, 2019
Export Citation:
Assignee:
JSR CORP (JP)
UNIV KEIO (JP)
UNIV KEIO (JP)
International Classes:
C12N5/079; A61K35/30; A61P25/00; C12Q1/06
Domestic Patent References:
| WO2017060884A1 | 2017-04-13 | |||
| WO2014090993A1 | 2014-06-19 | |||
| WO2017121754A1 | 2017-07-20 | |||
| WO2015076388A1 | 2015-05-28 | |||
| WO1996022362A1 | 1996-07-25 | |||
| WO2002101057A1 | 2002-12-19 | |||
| WO1998030679A1 | 1998-07-16 |
Foreign References:
| JP2018214930A | 2018-11-15 | |||
| US5843780A | 1998-12-01 | |||
| US6200806B1 | 2001-03-13 | |||
| US6280718B1 | 2001-08-28 |
Other References:
LANCASTER M.A. ET AL.: "Cerebral organoids model human brain development and microcephaly", NATURE, vol. 501, no. 7467, 19 September 2013 (2013-09-19), pages 373 - 379, XP055576219, DOI: 10.1038/nature12517
MATSUI Y. ET AL.: "Derivation of pluripotential embryonic stem cells from murine primordial germ cells in culture", CELL, vol. 70, no. 5, 1992, pages 841 - 847, XP024245512, DOI: 10.1016/0092-8674(92)90317-6
OKITA K. ET AL.: "An efficient nonviral method to generate integration-free human-induced pluripotent stem cells from cord blood and peripheral blood cells", STEM CELLS, vol. 31, no. 3, 2013, pages 458 - 466, XP055461400, DOI: 10.1002/stem.1293
TAKAHASHI K.YAMANAKA S.: "Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors", CELL, vol. 126, no. 4, 2006, pages 663 - 676
TAKAHASHI K. ET AL.: "Induction of pluripotent stem cells from adult human fibroblasts by defined factors", CELL, vol. 131, no. 5, 2007, pages 861 - 872, XP055547222, DOI: 10.1016/j.cell.2007.11.019
YU J. ET AL.: "Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells", SCIENCE, vol. 318, no. 5858, 2007, pages 1917 - 1920, XP055435356, DOI: 10.1126/science.1151526
NAKAGAWA M. ET AL.: "Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts", NAT BIOTECHNOL, vol. 26, no. 1, 2008, pages 101 - 106, XP008153586, DOI: 10.1038/nbt1374
HOU P. ET AL.: "Pluripotent stem cells induced from mouse somatic cells by small-molecule compounds", SCIENCE, vol. 341, no. 6146, 2013, pages 651 - 654, XP055079363, DOI: 10.1126/science.1239278
HSU PD ET AL.: "Development and Applications of CRISPR-Cas9 for Genome Engineering", CELL, vol. 157, no. 6, 2014, pages 1262 - 1278, XP055694974, DOI: 10.1016/j.cell.2014.05.010
ISHIZAKI T. ET AL.: "Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases", MOL PHARMACOL, vol. 57, no. 5, 2000, pages 976 - 983, XP008003729
NARUMIYA S. ET AL.: "Use and properties of ROCK-specific inhibitor Y-27632", METHODS ENZYMOL, vol. 325, 2000, pages 273 - 284, XP008003728, DOI: 10.1016/S0076-6879(00)25449-9
UEHATA M. ET AL.: "Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase in hypertension", NATURE, vol. 389, no. 6654, 1997, pages 990 - 994, XP002229794, DOI: 10.1038/40187
SASAKI Y. ET AL.: "The novel and specific Rho-kinase inhibitor (S)-(+)-2-methyl-1-[(4-methyl-5-isoquinoline) sulfonyl]-homopiperazine as a probing molecule for Rho-kinase-involved pathway", PHARMACOL THER., vol. 93, no. 2-3, 2002, pages 225 - 232, XP002458450, DOI: 10.1016/S0163-7258(02)00191-2
NAKAJIMA M. ET AL.: "Effect of Wf-536, a novel ROCK inhibitor, against metastasis of B16 melanoma", CANCER CHEMOTHERAPY AND PHARMACOLOGY, vol. 52, no. 4, 2003, pages 319 - 324, XP055093569, DOI: 10.1007/s00280-003-0641-9
NAKAGAWA M. ET AL.: "A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells", SCIENTIFIC REPORTS, vol. 4, 2014, pages 3594
See also references of EP 3882342A4
MATSUI Y. ET AL.: "Derivation of pluripotential embryonic stem cells from murine primordial germ cells in culture", CELL, vol. 70, no. 5, 1992, pages 841 - 847, XP024245512, DOI: 10.1016/0092-8674(92)90317-6
OKITA K. ET AL.: "An efficient nonviral method to generate integration-free human-induced pluripotent stem cells from cord blood and peripheral blood cells", STEM CELLS, vol. 31, no. 3, 2013, pages 458 - 466, XP055461400, DOI: 10.1002/stem.1293
TAKAHASHI K.YAMANAKA S.: "Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors", CELL, vol. 126, no. 4, 2006, pages 663 - 676
TAKAHASHI K. ET AL.: "Induction of pluripotent stem cells from adult human fibroblasts by defined factors", CELL, vol. 131, no. 5, 2007, pages 861 - 872, XP055547222, DOI: 10.1016/j.cell.2007.11.019
YU J. ET AL.: "Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells", SCIENCE, vol. 318, no. 5858, 2007, pages 1917 - 1920, XP055435356, DOI: 10.1126/science.1151526
NAKAGAWA M. ET AL.: "Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts", NAT BIOTECHNOL, vol. 26, no. 1, 2008, pages 101 - 106, XP008153586, DOI: 10.1038/nbt1374
HOU P. ET AL.: "Pluripotent stem cells induced from mouse somatic cells by small-molecule compounds", SCIENCE, vol. 341, no. 6146, 2013, pages 651 - 654, XP055079363, DOI: 10.1126/science.1239278
HSU PD ET AL.: "Development and Applications of CRISPR-Cas9 for Genome Engineering", CELL, vol. 157, no. 6, 2014, pages 1262 - 1278, XP055694974, DOI: 10.1016/j.cell.2014.05.010
ISHIZAKI T. ET AL.: "Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases", MOL PHARMACOL, vol. 57, no. 5, 2000, pages 976 - 983, XP008003729
NARUMIYA S. ET AL.: "Use and properties of ROCK-specific inhibitor Y-27632", METHODS ENZYMOL, vol. 325, 2000, pages 273 - 284, XP008003728, DOI: 10.1016/S0076-6879(00)25449-9
UEHATA M. ET AL.: "Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase in hypertension", NATURE, vol. 389, no. 6654, 1997, pages 990 - 994, XP002229794, DOI: 10.1038/40187
SASAKI Y. ET AL.: "The novel and specific Rho-kinase inhibitor (S)-(+)-2-methyl-1-[(4-methyl-5-isoquinoline) sulfonyl]-homopiperazine as a probing molecule for Rho-kinase-involved pathway", PHARMACOL THER., vol. 93, no. 2-3, 2002, pages 225 - 232, XP002458450, DOI: 10.1016/S0163-7258(02)00191-2
NAKAJIMA M. ET AL.: "Effect of Wf-536, a novel ROCK inhibitor, against metastasis of B16 melanoma", CANCER CHEMOTHERAPY AND PHARMACOLOGY, vol. 52, no. 4, 2003, pages 319 - 324, XP055093569, DOI: 10.1007/s00280-003-0641-9
NAKAGAWA M. ET AL.: "A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells", SCIENTIFIC REPORTS, vol. 4, 2014, pages 3594
See also references of EP 3882342A4
Attorney, Agent or Firm:
MATSUNUMA Yasushi et al. (JP)
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