Title:
METHOD FOR PRODUCING CANCER STEM CELLS
Document Type and Number:
WIPO Patent Application WO/2018/151309
Kind Code:
A1
Abstract:
The present invention pertains to a method for producing cancer stem cells that includes obtaining a living cell population that contains cancer stem cells by culturing a living cell population that includes cancer cells in the joint presence of a gel substance, the gel substance being a material that induces the expression of osteopontin in at least some of the cells included in the living cell population. Furthermore, the present invention pertains to an inducer of the conversion of cancer cells into cancer stem cells, the inducer including a gel substance that induces the expression of osteopontin in at least some of the cells included in the living cell population. The gel substance comprises, for example, a double network gel, PNaSS gel, PCDME gel, PA gel, PAMPS gel, PDMA gel, or PAAc gel. Provided are a means and method with which it is possible to prepare cancer stem cells in a relatively short time at a relatively low culture cost without requiring expensive equipment.
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Inventors:
TANAKA SHINYA (JP)
YASUDA KAZUNORI (JP)
GONG JIAN PING (JP)
TSUDA MASUMI (JP)
KUROKAWA TAKAYUKI (JP)
YASUDA KAZUNORI (JP)
GONG JIAN PING (JP)
TSUDA MASUMI (JP)
KUROKAWA TAKAYUKI (JP)
Application Number:
PCT/JP2018/005884
Publication Date:
August 23, 2018
Filing Date:
February 20, 2018
Export Citation:
Assignee:
UNIV HOKKAIDO NAT UNIV CORP (JP)
International Classes:
C12N5/095; C12Q1/02
Foreign References:
| JP2016523079A | 2016-08-08 | |||
| US20150175972A1 | 2015-06-25 | |||
| JP2010158180A | 2010-07-22 |
Other References:
CAO, LEI ET AL.: "Osteopontin promotes a cancer stem cell - like phenotype in hepatocellular carcinoma cells via an integrin-NF-kB-HIF-la pathway", ONCOTARGET, vol. 6, no. 9, 2015, pages 6627 - 6640, XP055535323
MAEDA, EIJIRO ET AL.: "Significant increase in Young's modulus of ATDC5 cells during chondrogenic differentiation induced by PAMPS/PDMAAm double- network gel: Comparison with induction by insulin", JOURNAL OF BIOMECHANICS, vol. 47, no. 13, 2014, pages 3408 - 3414, XP029050399
TSUDA, MASUMI ET AL.: "Using biomateiral , cancer stem cell is made", CONSORTIUM OF BIOLOGICAL SCIENCES 2017, 8 December 2017 (2017-12-08)
MAEDA, EIJIRO ET AL.: "Significant increase in Young's modulus of ATDC5 cells during chondrogenic differentiation induced by PAMPS/PDMAAm double- network gel: Comparison with induction by insulin", JOURNAL OF BIOMECHANICS, vol. 47, no. 13, 2014, pages 3408 - 3414, XP029050399
TSUDA, MASUMI ET AL.: "Using biomateiral , cancer stem cell is made", CONSORTIUM OF BIOLOGICAL SCIENCES 2017, 8 December 2017 (2017-12-08)
Attorney, Agent or Firm:
SIKS & CO. (JP)
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