Title:
METHOD FOR PRODUCING CARDIOMYOCYTE POPULATION FROM PLURIPOTENT STEM CELLS
Document Type and Number:
WIPO Patent Application WO/2016/043168
Kind Code:
A1
Abstract:
Provided is a method for producing by a simple procedure in a short time a cardiomyocyte population having little damage that can be used safely in clinical practice, using a cell population that has been induced to differentiate from pluripotent stem cells into cardiomyocytes. The present invention is a method for producing a cardiomyocyte population including (1) a step for inducing pluripotent stem cells to differentiate into cardiomyocytes, (2) a step for bringing the cell population that has been induced to differentiate into contact with a laminin selected from the group consisting of laminin α2β1γ1, laminin α2β2γ1, laminin α1β1γ1, and laminin α1β2γ1 or a fragment having the integrin binding activity of this laminin, and (3) a step for collecting the cells adhered to the laminin or the fragment.
Inventors:
OCHI YUKIKO (JP)
SEKIGUCHI KIYOTOSHI (JP)
MIYAGAWA SHIGERU (JP)
SAWA YOSHIKI (JP)
SILTANEN ANTTI MARKUS (JP)
SEKIGUCHI KIYOTOSHI (JP)
MIYAGAWA SHIGERU (JP)
SAWA YOSHIKI (JP)
SILTANEN ANTTI MARKUS (JP)
Application Number:
PCT/JP2015/076072
Publication Date:
March 24, 2016
Filing Date:
September 14, 2015
Export Citation:
Assignee:
UNIV OSAKA (JP)
International Classes:
C12N5/077; C12N5/071; C12N5/10
Domestic Patent References:
| WO2014014119A1 | 2014-01-23 |
Foreign References:
| JP2008543338A | 2008-12-04 | |||
| JP2014511139A | 2014-05-12 | |||
| JP2011078370A | 2011-04-21 |
Other References:
ZIOBER, B.L. ET AL.: "Alternative Extracellular and Cytoplasmic Domains of the Integrin a7 Subunit Are Differentially Expressed During Development", J. BIOL. CHEM., vol. 268, no. 35, 1993, pages 26773 - 26783, XP055418138
LEUNG, E. ET AL.: "A Novel Extracellular Domain Variant of the Human Integrin a7 Subunit Generated by Alternative Intron Splicing", BIOCHEM. BIOPHYS. RES. COMMUN., vol. 243, 1998, pages 317 - 325, XP055418167
MAITRA, N. ET AL.: "Expression of a and beta integrins during terminal differentiation of cardiomyocytes", CARDIOVASCULAR RESEARCH, vol. 47, 2000, pages 715 - 725, XP055418142
VON DER MARK, H. ET AL.: "Alternative Splice Variants of alpha7beta1 Integrin Selectively Recognize Different Laminin Isoforms", J. BIOL. CHEM., vol. 277, no. 8, 2002, pages 6012 - 6016, XP055418145
RYOKO NISHIUCHI ET AL.: "Saibo Secchaku Inshi no Seibutsugaku", TISSUE ENGINEERING 2007, 27 June 2007 (2007-06-27), pages 88 - 95, XP008157427
NISHIUCHI R. ET AL.: "Ligand-binding specificities of laminin-binding integrins: A comprehensive survey of laminin-integrin interactions using recombinant alpha3beta1, alpha6beta1, alpha7beta1 and alpha6beta4 integrins", MATRIX BIOLOGY, vol. 25, 2006, pages 189 - 197, XP027933625, DOI: doi:10.1016/j.matbio.2005.12.001
TANIGUCHI, Y. ET AL.: "The C-terminal Region of Laminin beta Chains Modulates the Integrin Binding Affinities of Laminins", J. BIOL. CHEM., vol. 284, no. 12, 20 March 2009 (2009-03-20), pages 7820 - 7831, XP055022477, DOI: doi:10.1074/jbc.M809332200
MIYAZAKI T. ET AL.: "Recombinant human laminin isoforms can support the undifferentiated growth of human embryonic stem cells", BIOCHEM. BIOPHYS. RES. COMMUN., vol. 375, 2008, pages 27 - 32, XP024520467, DOI: doi:10.1016/j.bbrc.2008.07.111
SINGH, A. ET AL.: "Adhesion strength-based, label-free isolation of human pluripotent stem cells", NATURE, vol. 10, no. 5, 2013, pages 438 - 444, XP055418153
LEE, S.T. ET AL.: "Development of three dimensional culture and expression of integrin heterodimers in human embryonic stem cells", ORGANOGENESIS, vol. 9, no. 3, 2013, pages 143 - 148, XP055418160
YUKIKO IMANISHI ET AL.: "Hito iPS Yurai Shinkin Saibo ni Okeru Laminin Mochiita Saibo Seiseiho no Kento", REGENERATIVE MEDICINE, vol. 14, 1 February 2015 (2015-02-01), pages 355, XP009501003
LEUNG, E. ET AL.: "A Novel Extracellular Domain Variant of the Human Integrin a7 Subunit Generated by Alternative Intron Splicing", BIOCHEM. BIOPHYS. RES. COMMUN., vol. 243, 1998, pages 317 - 325, XP055418167
MAITRA, N. ET AL.: "Expression of a and beta integrins during terminal differentiation of cardiomyocytes", CARDIOVASCULAR RESEARCH, vol. 47, 2000, pages 715 - 725, XP055418142
VON DER MARK, H. ET AL.: "Alternative Splice Variants of alpha7beta1 Integrin Selectively Recognize Different Laminin Isoforms", J. BIOL. CHEM., vol. 277, no. 8, 2002, pages 6012 - 6016, XP055418145
RYOKO NISHIUCHI ET AL.: "Saibo Secchaku Inshi no Seibutsugaku", TISSUE ENGINEERING 2007, 27 June 2007 (2007-06-27), pages 88 - 95, XP008157427
NISHIUCHI R. ET AL.: "Ligand-binding specificities of laminin-binding integrins: A comprehensive survey of laminin-integrin interactions using recombinant alpha3beta1, alpha6beta1, alpha7beta1 and alpha6beta4 integrins", MATRIX BIOLOGY, vol. 25, 2006, pages 189 - 197, XP027933625, DOI: doi:10.1016/j.matbio.2005.12.001
TANIGUCHI, Y. ET AL.: "The C-terminal Region of Laminin beta Chains Modulates the Integrin Binding Affinities of Laminins", J. BIOL. CHEM., vol. 284, no. 12, 20 March 2009 (2009-03-20), pages 7820 - 7831, XP055022477, DOI: doi:10.1074/jbc.M809332200
MIYAZAKI T. ET AL.: "Recombinant human laminin isoforms can support the undifferentiated growth of human embryonic stem cells", BIOCHEM. BIOPHYS. RES. COMMUN., vol. 375, 2008, pages 27 - 32, XP024520467, DOI: doi:10.1016/j.bbrc.2008.07.111
SINGH, A. ET AL.: "Adhesion strength-based, label-free isolation of human pluripotent stem cells", NATURE, vol. 10, no. 5, 2013, pages 438 - 444, XP055418153
LEE, S.T. ET AL.: "Development of three dimensional culture and expression of integrin heterodimers in human embryonic stem cells", ORGANOGENESIS, vol. 9, no. 3, 2013, pages 143 - 148, XP055418160
YUKIKO IMANISHI ET AL.: "Hito iPS Yurai Shinkin Saibo ni Okeru Laminin Mochiita Saibo Seiseiho no Kento", REGENERATIVE MEDICINE, vol. 14, 1 February 2015 (2015-02-01), pages 355, XP009501003
Attorney, Agent or Firm:
IWATANI, Ryo (JP)
Tatsu Iwatani (JP)
Tatsu Iwatani (JP)
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