Title:
METHOD FOR PRODUCING CELL MASS INCLUDING PITUITARY TISSUE, AND CELL MASS THEREOF
Document Type and Number:
WIPO Patent Application WO/2019/103129
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a technique for efficiently producing a cell mass including pituitary tissue from pluripotent stem cells. A method for producing a cell mass including pituitary tissue, the method including the following steps (1) and (2): (1) a first step for suspension-culturing pluripotent stem cells in the presence of a Wnt signaling pathway inhibitor, and forming an aggregate of cells; and (2) a second step for suspension-culturing the aggregate obtained in the first step in the presence of a BMP signaling pathway agonist and a sonic hedgehog signaling pathway agonist, and obtaining a cell mass including pituitary tissue.
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Inventors:
NAKANO TOKUSHIGE (JP)
Application Number:
PCT/JP2018/043290
Publication Date:
May 31, 2019
Filing Date:
November 22, 2018
Export Citation:
Assignee:
SUMITOMO CHEMICAL CO (JP)
International Classes:
C12N5/0735; A61K35/30; A61P5/06; A61P5/10; A61P43/00; C12N5/079; C12N5/10
Domestic Patent References:
| WO2016013669A1 | 2016-01-28 | |||
| WO2017126551A1 | 2017-07-27 | |||
| WO2017183732A1 | 2017-10-26 | |||
| WO2013065763A1 | 2013-05-10 | |||
| WO2016063985A1 | 2016-04-28 | |||
| WO2009148170A1 | 2009-12-10 | |||
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| JP2016538856A | 2016-12-15 | |||
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OZONE C. ET AL.: "Functional anterior pituitary generated in self-organizing culture of human embryonic stem cells", FUNCTIONAL ANTERIOR PITUITARY GENERATED IN SELF-ORGANIZING CULTURE OF HUMAN EMBRYONIC STEM CELLS, vol. 7, no. 1, January 2016 (2016-01-01), pages 1 - 10, XP055535023
DINCER ET AL., CELL REPORTS, vol. 5, 2013, pages 1387 - 1402
ZIMMER ET AL., STEM CELL REPORTS, vol. 6, 2016, pages 858 - 872
CELL, vol. 70, 1992, pages 841 - 847
YAMANAKA ET AL., MOUSE CELL, 2006
CELL, vol. 126, no. 4, 2006, pages 663 - 676
CELL, vol. 131, no. 5, 2007, pages 861 - 872
SCIENCE, vol. 318, no. 5858, 2007, pages 1917 - 1920
NAT. BIOTECHNOL., vol. 26, no. 1, 2008, pages 101 - 106
SCIENCE, vol. 341, 2013, pages 651 - 654
"Manipulating the Mouse Embryo, A Laboratory Manual", 1994, COLD SPRING HARBOR LABORATORY PRESS
"Gene Targeting, A Practical Approach", 1993, IRL PRESS AT OXFORD UNIVERSITY PRESS
"Biomanual Series 8, Gene Targeting, Making of Mutant Mouse using ES cell", 1995, YODOSHA CO., LTD.
"Molecular Cloning, A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
J. CELL BIOL., vol. 105, 1987, pages 589 - 598
CELL STEM CELL, vol. 10, no. 6, 2012, pages 771 - 775
NATURE METHODS, vol. 8, 2011, pages 424 - 429
SCIENTIFIC REPORTS, vol. 4, 2014, pages 3594
NAT BIOTECHNOL, vol. 28, 2010, pages 611 - 615
NAT COMMUN, vol. 3, 2012, pages 1236
NAT BIOTECHNOL, vol. 19, 2001, pages 971 - 974
EMBO J., vol. 3, 1984, pages 1463 - 1468
ONCOGENE, vol. 26, 2007, pages 5163 - 5168
AM J CANCER RES., vol. 5, no. 8, 2015, pages 2344 - 2360
DEV BIOL., vol. 361, no. 2, 15 January 2012 (2012-01-15), pages 392 - 402
BIOCHEM J., vol. 402, 15 March 2007 (2007-03-15), pages 515 - 523
ONCO TARGETS THER., vol. 6, 2013, pages 943 - 955
Attorney, Agent or Firm:
TAKASHIMA, Hajime et al. (JP)
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