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Title:
METHOD FOR PRODUCING COMPREHENSIVE ANTI-SURFACE ANTIBODY WHEREIN THE ANTIGEN USED IS A MICROORGANISM FIXED BY A PROTEIN CROSSLINKING AND FIXATION REAGENT
Document Type and Number:
WIPO Patent Application WO/2010/071237
Kind Code:
A1
Abstract:
Provided is a method for producing comprehensive antibody against an entire protein expressed at the surface of a microorganism, and the comprehensive antibody obtained by the same method.  The method for producing comprehensive antibody that contains antibody to an entire protein molecule expressed on the surface of a microorganism comprises (a) treatment of a microorganism using a protein crosslinking and fixation reagent in order to fix the protein expressed at the surface of a microorganism by intramolecular crosslinking, (b) immunogen administration to a laboratory animal using as the immunogen the microorganism that has been fixed by the protein crosslinking and fixation reagent, and c) recovery of antibody from the laboratory animal. 

Inventors:
SHICHIRI, Masayoshi (1-27-1-1206, Wakagi Itabashi-k, Tokyo 65, 〒1740065, JP)
Application Number:
JP2009/071716
Publication Date:
June 24, 2010
Filing Date:
December 18, 2009
Export Citation:
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Assignee:
KABUSHIKI KAISHA SAIWAI MEDIX (Kubota Bldg. 1F, 1-12-17 Kandaizumi-cho, Chiyoda-k, Tokyo 24, 〒1010024, JP)
株式会社さいわいメディックス (〒24 東京都千代田区神田和泉町1番地12-17 久保田ビル1F Tokyo, 〒1010024, JP)
SHICHIRI, Masayoshi (1-27-1-1206, Wakagi Itabashi-k, Tokyo 65, 〒1740065, JP)
七里眞義 (〒65 東京都板橋区若木1-27-1-1206 Tokyo, 〒1740065, JP)
II, Ikuko (894-13, Horinishi Hadano-sh, Kanagawa 31, 〒2591331, JP)
井伊郁子 (〒31 神奈川県秦野市堀西894-13 Kanagawa, 〒2591331, JP)
International Classes:
C07K16/02; A23K1/18; A23L1/305; A61K39/395; A61P31/00; C07K16/12
Foreign References:
JP2008324257A2008-12-19
Other References:
TERUO MIYAZAKI: 'Keiran Kotai no Gyorui Kansensho Bojo eno Riyo' JOURNAL OF THE AGRICULTURAL CHEMICAL SOCIETY OF JAPAN vol. 67, 1993, pages 1440 - 1443, XP008159742
NYGREN E. ET AL.: 'Murine antibody responses following systemic or mucosal immunization with viable or inactivated Vibrio cholerae.' VACCINE vol. 26, 09 December 2008, pages 6784 - 6790
TETI G. ET AL.: 'Specificity and protective activity of murine monoclonal antibodies directed against the capsular polysaccharide of type III group B streptococci.' HYBRIDOMA vol. 11, 1992, pages 13 - 22
MAKOTO SHIMIZU: 'Keiran Ran'o Chu ni Chikuseki suru Kotai - sono Tokusei to Riyo Niwatori Yurai IgG Kotai = IgY no Shokuhin, Shiyaku eno Oyo ni Kitai' KAGAKU TO SEIBUTSU vol. 30, 1992, pages 492 - 494
TSUKASA SUGIYAMA: 'Judo Men'eki Shokuhin to shite no Tamago -Keiran Kotai (IgY) to sono Riyo ni Tsuite' NOGYO GIJUTSU KENKYU vol. 50, no. 5, 1996, pages 86 - 87
KAZUNORI NAKAGAWA ET AL.: 'Niwatori o Mochiita Kotai no Sakuseiho' KESSEN SHIKETSUSHI vol. 9, 1998, pages 435 - 439
HIROAKI KINTSUJI ET AL.: '17) Hapten-ka Oyobi Men'eki Gensei Kyoka shita Reisuibyokintai Shinshi Vaccine no Yukosei' HEISEI 14 NENDO SHIGA-KEN SUISAN SHIKENJO JIGYO HOKOKU 2002, pages 188 - 189
See also references of EP 2377885A1
Attorney, Agent or Firm:
HIRAKI, Yusuke et al. (Kamiya-cho MT Bldg. 19F, 3-20 Toranomon 4-chome, Minato-k, Tokyo 01, 〒1050001, JP)
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