Title:
METHOD FOR PRODUCING CORNEAL ENDOTHELIAL CELL
Document Type and Number:
WIPO Patent Application WO/2013/051722
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide: a method for producing a corneal endothelial cell, particularly from a neural crest stem cell originated from a parenchyma of cornea or an iPS cell, with high efficiency; a method for producing a corneal endothelial cell in a large quantity in a steady manner by inducing the differentiation of a stem cell into the corneal endothelial cell more efficiently; and a medicinal agent comprising a corneal endothelial cell.
A method for inducing the differentiation of a corneal endothelial cell from a stem cell, comprising a step of culturing the stem cell in a differentiation-inducing culture medium containing a GSK3 inhibitor (preferably a GSK3β inhibitor) and retinoic acid, preferably additionally containing at least one component selected from the group consisting of TGFb2, insulin and a ROCK inhibitor; and others.
Inventors:
SHIMMURA SHIGETO (JP)
HATOU SHIN (JP)
TSUBOTA KAZUO (JP)
YOSHIDA SATORU (JP)
HATOU SHIN (JP)
TSUBOTA KAZUO (JP)
YOSHIDA SATORU (JP)
Application Number:
PCT/JP2012/076048
Publication Date:
April 11, 2013
Filing Date:
October 05, 2012
Export Citation:
Assignee:
UNIV KEIO (JP)
International Classes:
C12N5/10; A61K35/44; A61L27/00; A61P27/02; C12N15/09
Foreign References:
| JP2009268433A | 2009-11-19 | |||
| JP2004024852A | 2004-01-29 | |||
| JP2005229869A | 2005-09-02 | |||
| JP2009268433A | 2009-11-19 |
Other References:
SHIN HATO ET AL.: "Kakumaku Jisshitsu Kansaibo kara Kakumaku Naihi Saibo eno Bunka Yudo", REGENERATIVE MEDICINE, vol. 10, 1 February 2011 (2011-02-01), pages 163, XP008173359
SHIN HATO ET AL.: "Kakumaku Jisshitsu Kansaibo kara Kakumaku Naihi Saibo eno Bunka Yudo", JAPAN CORNEA SOCIETY SOKAI · KERATOPLASTY SOCIETY OF JAPAN PROGRAM SHOROKUSHU, vol. 35TH-27T, February 2011 (2011-02-01), pages 59, XP008173306
YOSHIDA,S. ET AL.: "Isolation of multipotent neural crest-derived stem cells from the adult mouse cornea", STEM CELLS, vol. 24, no. 12, December 2006 (2006-12-01), pages 2714 - 22, XP055142565
TOMA,J.G. ET AL.: "Isolation of multipotent adult stem cells from the dermis of mammalian skin", NAT. CELL BIOL., vol. 3, no. 9, September 2001 (2001-09-01), pages 778 - 84, XP001022251
LEE,G. ET AL.: "Derivation of neural crest cells from human pluripotent stem cells", NAT. PROTOC., vol. 5, no. 4, April 2010 (2010-04-01), pages 688 - 701, XP009162965
AGGARWAL RK, BR J OPHTHALMOL, vol. 81, 1997, pages 178 - 179
SHIMMURA S. ET AL., CORNEA, vol. 24, no. 2, 2005, pages 178 - 181
PRICE FW JR; PRICE MO, J REFRACT SURG., vol. 21, no. 4, 2005, pages 339 - 345
YOSHIDA S. ET AL., STEM CELLS, vol. 24, no. 12, 2006, pages 2714 - 2722
NAT BIOTECHNOL, vol. 26, 2008, pages 101 - 106
NATURE, vol. 454, no. 7204, 31 July 2008 (2008-07-31), pages 646 - 50
CELL STEM CELL, vol. 4, no. 1, 9 January 2009 (2009-01-09), pages 16 - 9
CELL STEM CELL, vol. 5, no. 5, 6 November 2009 (2009-11-06), pages 491 - 503
CELL STEM CELL., vol. 4, no. 5, 8 May 2009 (2009-05-08), pages 381 - 4
NATURE PROTOCOLS, vol. 5, no. 4, 2010, pages 688 - 701
NAT CELL BIOL., vol. 3, 2001, pages 778 - 784
INVESTIGATIVE OPHTHLMOLOGY & VISUAL SCIENCE, vol. 51, no. 8, 2010, pages 3935 - 3942
CURRENT EYE RESEARCH, vol. 34, 2009, pages 347 - 354
NAT CELL BIOL., vol. 3, 2001, pages 778 784
SHIN HATO ET AL.: "Kakumaku Jisshitsu Kansaibo kara Kakumaku Naihi Saibo eno Bunka Yudo", JAPAN CORNEA SOCIETY SOKAI · KERATOPLASTY SOCIETY OF JAPAN PROGRAM SHOROKUSHU, vol. 35TH-27T, February 2011 (2011-02-01), pages 59, XP008173306
YOSHIDA,S. ET AL.: "Isolation of multipotent neural crest-derived stem cells from the adult mouse cornea", STEM CELLS, vol. 24, no. 12, December 2006 (2006-12-01), pages 2714 - 22, XP055142565
TOMA,J.G. ET AL.: "Isolation of multipotent adult stem cells from the dermis of mammalian skin", NAT. CELL BIOL., vol. 3, no. 9, September 2001 (2001-09-01), pages 778 - 84, XP001022251
LEE,G. ET AL.: "Derivation of neural crest cells from human pluripotent stem cells", NAT. PROTOC., vol. 5, no. 4, April 2010 (2010-04-01), pages 688 - 701, XP009162965
AGGARWAL RK, BR J OPHTHALMOL, vol. 81, 1997, pages 178 - 179
SHIMMURA S. ET AL., CORNEA, vol. 24, no. 2, 2005, pages 178 - 181
PRICE FW JR; PRICE MO, J REFRACT SURG., vol. 21, no. 4, 2005, pages 339 - 345
YOSHIDA S. ET AL., STEM CELLS, vol. 24, no. 12, 2006, pages 2714 - 2722
NAT BIOTECHNOL, vol. 26, 2008, pages 101 - 106
NATURE, vol. 454, no. 7204, 31 July 2008 (2008-07-31), pages 646 - 50
CELL STEM CELL, vol. 4, no. 1, 9 January 2009 (2009-01-09), pages 16 - 9
CELL STEM CELL, vol. 5, no. 5, 6 November 2009 (2009-11-06), pages 491 - 503
CELL STEM CELL., vol. 4, no. 5, 8 May 2009 (2009-05-08), pages 381 - 4
NATURE PROTOCOLS, vol. 5, no. 4, 2010, pages 688 - 701
NAT CELL BIOL., vol. 3, 2001, pages 778 - 784
INVESTIGATIVE OPHTHLMOLOGY & VISUAL SCIENCE, vol. 51, no. 8, 2010, pages 3935 - 3942
CURRENT EYE RESEARCH, vol. 34, 2009, pages 347 - 354
NAT CELL BIOL., vol. 3, 2001, pages 778 784
Attorney, Agent or Firm:
TAKASHIMA, HAJIME (JP)
Takashima 1 (JP)
Takashima 1 (JP)
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