Title:
METHOD FOR PRODUCING CULTURED CELL, AND METHOD FOR PRODUCING THERAPEUTIC AGENT FOR SPINAL CORD INJURY DISEASE
Document Type and Number:
WIPO Patent Application WO/2019/092939
Kind Code:
A1
Abstract:
[Problem] To provide a cell culture method which can achieve the increase in safe expression of GDNF gene (mRNA) without needing to employ a gene transfer method using a virus.
[Solution] A method for producing a cultured cell containing mRNA for glial cell line derived neurotrophic factor (GDNF), the method including a culture step of culturing a human skin-derived stem cell in a serum-free culture medium containing at least one component selected from SAG, purmorphamine and sonic hedgehog (SHH) protein, wherein the serum-free culture medium may additionally contain a B-27 supplement, a ROCK inhibitor, EGF and FGF2.
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Inventors:
NISHINA HIROMICHI (JP)
YANAGA HIROKO (JP)
YANAGA HIROKO (JP)
Application Number:
PCT/JP2018/028998
Publication Date:
May 16, 2019
Filing Date:
August 02, 2018
Export Citation:
Assignee:
REGENESIS SCIENCE CO LTD (JP)
International Classes:
C12N15/01; A61K35/30; A61K35/36; A61P25/00; C12N1/00; C12N5/10; C12N5/074
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Other References:
CHEN, J, K. ET AL.: "Small molecule modulation of Smoothened activity", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 99, no. 22, 29 October 2002 (2002-10-29), pages 14071 - 14076, XP002251705
CHEN, W. ET AL.: "Activity-dependent internalization of smoothened mediated by beta-arrestin 2 and GRK2.", SCIENCE, vol. 306, no. 5705, 24 December 2004 (2004-12-24), pages 2257 - 2260, XP055607826
TSUJI O ET AL.: "Therapeutic potential of appropriately evaluated self-induced pluripotent stem cells for spinal cord injury", PNAS, vol. 107, no. 28, 2010, pages 12704 - 12709
INOUE H ET AL.: "Improvement of hind-paralysis following traumatic spinal cord injury in rats by grafting normal human keratinocytes: new cell-therapy strategy for nerve regeneration", J ARTIF ORGANS, vol. 14, no. 4, 2011, pages 375 - 80, XP019987750, DOI: 10.1007/s10047-011-0598-0
INOUE H ET AL.: "Improvement of hind-limb paralysis following traumatic spinal cord injury in rats by grafting normal human keratinocytes: new cell-therapy strategy for nerve regeneration", J ARTIF ORGANS, vol. 14, no. 4, 2011, pages 375 - 80, XP019987750, DOI: 10.1007/s10047-011-0598-0
ANSORENA E ET AL.: "Injectable alginate hydrogel loaded with GDNF promotes functional recovery in a hemisection model of spinal cord injury", INT J PHARM., vol. 455, no. 1-2, 2013, pages 148 - 58, XP028699749, DOI: 10.1016/j.ijpharm.2013.07.045
TANG XQ ET AL.: "Adenovirus-mediated delivery of GDNF ameliorates corticospinal neuronal atrophy and motor function deficits in rats with spinal cord injury", NEUROREPORT, vol. 15, no. 3, 2004, pages 425 - 9
GUZEN FP ET AL.: "Glial cell line-derived neurotrophic factor added to a sciatic nerve fragment grafted in a spinal cord gap ameliorates motor impairments in rats and increases local axonal growth", RESTOR NEUROL NEUROSCI, vol. 27, no. 1, 2009, pages 1 - 16
KAO CH ET AL.: "Exogenous administration of glial cell line-derived neurotrophic factor improves recovery after spinal cord injury", RESUSCITATION, vol. 77, no. 3, 2008, pages 395 - 400, XP022667026, DOI: 10.1016/j.resuscitation.2008.01.023
KOELSCH A ET AL.: "Transgene-mediated GDNF expression enhances synaptic connectivity and GAB A transmission to improve functional outcome after spinal cord contusion", J NEUROCHEM, vol. 113, no. 1, 2010, pages 143 - 52
CAO L ET AL.: "Olfactory ensheathing cells genetically modified to secrete GDNF to promote spinal cord repair", BRAIN, vol. 127, March 2004 (2004-03-01), pages 535 - 49
DENG LX ET AL.: "A novel growth-promoting pathway formed by GDNF-overexpressing Schwann cells promotes propriospinal axonal regeneration, synapse formation, and partial recovery of function after spinal cord injury", J NEUROSCI, vol. 33, no. 13, 27 March 2013 (2013-03-27), pages 5655 - 67
STEPHEN J. A. DAVIEES ET AL.: "Transplantaion of specific human astrocytes promotes functional recovery after spinal cord injury", PLOS ONE, vol. 6, no. 3, 2011, pages e17328
SOWA NA ET AL.: "Ecto-5'-nucleotidase (CD73) inhibits nociception by hydrolyzing AMP to adenosine in nociceptive circuits", J NEUROSCI, vol. 30, no. 6, 10 February 2010 (2010-02-10), pages 2235 - 2244
KIM MS ET AL.: "Perspectives on Tissue-Engineered Nerve Regeneration for the Treatment of Spinal Cord Injury", TISSUE ENG PART, vol. 20, no. 13-14, 2014, pages 1781 - 3
BIEMASKIE J ET AL.: "Skin-derived precursors generate myelinating Schwann cells that promote remyelination and functional recovery after contusion spinal cord injury", J NEUROSCIENCE, vol. 27, no. 36, 2007, pages 9545 - 9559, XP055331841, DOI: 10.1523/JNEUROSCI.1930-07.2007
ZHAO YZ ET AL.: "Thermosensitive heparin-poloxamer hydrogels enhance the effects of GDNF on neuronal circuit remodeling and neuroprotection after spinal cord injury", J BIOMED MATER RES A, vol. 5, no. 10, 2017, pages 2816 - 2829
ZHOU HL ET AL.: "Changes in Glial cell line-derived neurotrophic factor expression in the rostral and caudal stumps of the transected adult rat spinal cord", NEUROCHEM RES, vol. 33, no. 5, 2008, pages 927 - 37, XP019579149
CHOU AK ET AL.: "Adenoviral-mediated glial cell line-derived neurotrophic factor gene transfer has a protective effect on sciatic nerve following constriction-induced spinal cord injury", PLOS ONE, vol. 9, no. 3, 2014, pages e92264
DETLOFF MR ET AL.: "Acute exercise prevents the development of neuropathic pain and the sprouting of non-peptidergic (GDNF- and artemin-responsive) c-fibers after spinal cord injury", EXP NEUROL, vol. 255, 2014, pages 38 - 48
DOLBEARE D ET AL.: "Restriction of axonal retraction and promotion of axonal regeneration by chronically injured neurons after intraspinal treatment with glial cell line-derived neurotrophic factor (GDNF", J NEUROTRAUMA, vol. 20, no. 11, 2003, pages 1251 - 61
LU Y ET AL.: "Glial cell line-derived neurotrophic factor-transfected placenta-derived versus bone marrow-derived mesenchymal cells for treating spinal cord injury", MED SCI MONIT, vol. 23, 2017, pages 1800 - 1811
CODEGA P ET AL.: "Prospective identification and purification of quiescent adult neural stem cells from their in vivo niche", NEURON, vol. 82, no. 3, 2014, pages 545 - 59, XP028654862, DOI: 10.1016/j.neuron.2014.02.039
DECAROLIS NA ET AL.: "In vivo contribution of nestin-and GLAST-lineage cells to adult hippocampal neurogenesis", HIPPOCAMPUS, vol. 23, no. 8, 2013, pages 708 - 719
SUFAN WU ET AL.: "Bone marrow stromal cells enhance differentiation of cocultured neurosphere cells and promote regeneration of injured spinal cord", J NEUROSCI RES, vol. 72, no. 3, 2003, pages 343 - 51
CHEMICAL ABSTRACTS, Columbus, Ohio, US; abstract no. 364590-63-6
SINHA SCHEN JK, NAT CHEM BIOL., vol. 2, no. 1, January 2006 (2006-01-01), pages 29 - 30
CHEN JKTAIPALE JYOUNG KEMAITI TBEACHY PA, PROC NATL ACAD SCI USA., vol. 99, no. 22, 29 October 2002 (2002-10-29), pages 14071 - 6
CHEN, W.REN, X. R.NELSON, C. D.BARAK, L. S.CHEN, J. K.BEACHY, P. A.DE SAUVAGE, F.LEFKOWITZ, R. J., SCIENCE, vol. 306, no. 5705, 2004, pages 2257 - 2260
ISHIZAKI ET AL., MOL. PHARMACOL., vol. 57, 2000, pages 976 - 983
NARUMIYA ET AL., METHODS ENZYMOL., vol. 325, 2000, pages 273 - 284
UENATA ET AL., NATURE, vol. 389, 1997, pages 990 - 994
SASAKI ET AL., PHARMACOL. THER., vol. 93, 2002, pages 225 - 232
NAKAJIMA ET AL., CANCER CHEMOTHER. PHARMACOL., vol. 52, no. 4, 2003, pages 319 - 324
See also references of EP 3708662A4
CHEN, W. ET AL.: "Activity-dependent internalization of smoothened mediated by beta-arrestin 2 and GRK2.", SCIENCE, vol. 306, no. 5705, 24 December 2004 (2004-12-24), pages 2257 - 2260, XP055607826
TSUJI O ET AL.: "Therapeutic potential of appropriately evaluated self-induced pluripotent stem cells for spinal cord injury", PNAS, vol. 107, no. 28, 2010, pages 12704 - 12709
INOUE H ET AL.: "Improvement of hind-paralysis following traumatic spinal cord injury in rats by grafting normal human keratinocytes: new cell-therapy strategy for nerve regeneration", J ARTIF ORGANS, vol. 14, no. 4, 2011, pages 375 - 80, XP019987750, DOI: 10.1007/s10047-011-0598-0
INOUE H ET AL.: "Improvement of hind-limb paralysis following traumatic spinal cord injury in rats by grafting normal human keratinocytes: new cell-therapy strategy for nerve regeneration", J ARTIF ORGANS, vol. 14, no. 4, 2011, pages 375 - 80, XP019987750, DOI: 10.1007/s10047-011-0598-0
ANSORENA E ET AL.: "Injectable alginate hydrogel loaded with GDNF promotes functional recovery in a hemisection model of spinal cord injury", INT J PHARM., vol. 455, no. 1-2, 2013, pages 148 - 58, XP028699749, DOI: 10.1016/j.ijpharm.2013.07.045
TANG XQ ET AL.: "Adenovirus-mediated delivery of GDNF ameliorates corticospinal neuronal atrophy and motor function deficits in rats with spinal cord injury", NEUROREPORT, vol. 15, no. 3, 2004, pages 425 - 9
GUZEN FP ET AL.: "Glial cell line-derived neurotrophic factor added to a sciatic nerve fragment grafted in a spinal cord gap ameliorates motor impairments in rats and increases local axonal growth", RESTOR NEUROL NEUROSCI, vol. 27, no. 1, 2009, pages 1 - 16
KAO CH ET AL.: "Exogenous administration of glial cell line-derived neurotrophic factor improves recovery after spinal cord injury", RESUSCITATION, vol. 77, no. 3, 2008, pages 395 - 400, XP022667026, DOI: 10.1016/j.resuscitation.2008.01.023
KOELSCH A ET AL.: "Transgene-mediated GDNF expression enhances synaptic connectivity and GAB A transmission to improve functional outcome after spinal cord contusion", J NEUROCHEM, vol. 113, no. 1, 2010, pages 143 - 52
CAO L ET AL.: "Olfactory ensheathing cells genetically modified to secrete GDNF to promote spinal cord repair", BRAIN, vol. 127, March 2004 (2004-03-01), pages 535 - 49
DENG LX ET AL.: "A novel growth-promoting pathway formed by GDNF-overexpressing Schwann cells promotes propriospinal axonal regeneration, synapse formation, and partial recovery of function after spinal cord injury", J NEUROSCI, vol. 33, no. 13, 27 March 2013 (2013-03-27), pages 5655 - 67
STEPHEN J. A. DAVIEES ET AL.: "Transplantaion of specific human astrocytes promotes functional recovery after spinal cord injury", PLOS ONE, vol. 6, no. 3, 2011, pages e17328
SOWA NA ET AL.: "Ecto-5'-nucleotidase (CD73) inhibits nociception by hydrolyzing AMP to adenosine in nociceptive circuits", J NEUROSCI, vol. 30, no. 6, 10 February 2010 (2010-02-10), pages 2235 - 2244
KIM MS ET AL.: "Perspectives on Tissue-Engineered Nerve Regeneration for the Treatment of Spinal Cord Injury", TISSUE ENG PART, vol. 20, no. 13-14, 2014, pages 1781 - 3
BIEMASKIE J ET AL.: "Skin-derived precursors generate myelinating Schwann cells that promote remyelination and functional recovery after contusion spinal cord injury", J NEUROSCIENCE, vol. 27, no. 36, 2007, pages 9545 - 9559, XP055331841, DOI: 10.1523/JNEUROSCI.1930-07.2007
ZHAO YZ ET AL.: "Thermosensitive heparin-poloxamer hydrogels enhance the effects of GDNF on neuronal circuit remodeling and neuroprotection after spinal cord injury", J BIOMED MATER RES A, vol. 5, no. 10, 2017, pages 2816 - 2829
ZHOU HL ET AL.: "Changes in Glial cell line-derived neurotrophic factor expression in the rostral and caudal stumps of the transected adult rat spinal cord", NEUROCHEM RES, vol. 33, no. 5, 2008, pages 927 - 37, XP019579149
CHOU AK ET AL.: "Adenoviral-mediated glial cell line-derived neurotrophic factor gene transfer has a protective effect on sciatic nerve following constriction-induced spinal cord injury", PLOS ONE, vol. 9, no. 3, 2014, pages e92264
DETLOFF MR ET AL.: "Acute exercise prevents the development of neuropathic pain and the sprouting of non-peptidergic (GDNF- and artemin-responsive) c-fibers after spinal cord injury", EXP NEUROL, vol. 255, 2014, pages 38 - 48
DOLBEARE D ET AL.: "Restriction of axonal retraction and promotion of axonal regeneration by chronically injured neurons after intraspinal treatment with glial cell line-derived neurotrophic factor (GDNF", J NEUROTRAUMA, vol. 20, no. 11, 2003, pages 1251 - 61
LU Y ET AL.: "Glial cell line-derived neurotrophic factor-transfected placenta-derived versus bone marrow-derived mesenchymal cells for treating spinal cord injury", MED SCI MONIT, vol. 23, 2017, pages 1800 - 1811
CODEGA P ET AL.: "Prospective identification and purification of quiescent adult neural stem cells from their in vivo niche", NEURON, vol. 82, no. 3, 2014, pages 545 - 59, XP028654862, DOI: 10.1016/j.neuron.2014.02.039
DECAROLIS NA ET AL.: "In vivo contribution of nestin-and GLAST-lineage cells to adult hippocampal neurogenesis", HIPPOCAMPUS, vol. 23, no. 8, 2013, pages 708 - 719
SUFAN WU ET AL.: "Bone marrow stromal cells enhance differentiation of cocultured neurosphere cells and promote regeneration of injured spinal cord", J NEUROSCI RES, vol. 72, no. 3, 2003, pages 343 - 51
CHEMICAL ABSTRACTS, Columbus, Ohio, US; abstract no. 364590-63-6
SINHA SCHEN JK, NAT CHEM BIOL., vol. 2, no. 1, January 2006 (2006-01-01), pages 29 - 30
CHEN JKTAIPALE JYOUNG KEMAITI TBEACHY PA, PROC NATL ACAD SCI USA., vol. 99, no. 22, 29 October 2002 (2002-10-29), pages 14071 - 6
CHEN, W.REN, X. R.NELSON, C. D.BARAK, L. S.CHEN, J. K.BEACHY, P. A.DE SAUVAGE, F.LEFKOWITZ, R. J., SCIENCE, vol. 306, no. 5705, 2004, pages 2257 - 2260
ISHIZAKI ET AL., MOL. PHARMACOL., vol. 57, 2000, pages 976 - 983
NARUMIYA ET AL., METHODS ENZYMOL., vol. 325, 2000, pages 273 - 284
UENATA ET AL., NATURE, vol. 389, 1997, pages 990 - 994
SASAKI ET AL., PHARMACOL. THER., vol. 93, 2002, pages 225 - 232
NAKAJIMA ET AL., CANCER CHEMOTHER. PHARMACOL., vol. 52, no. 4, 2003, pages 319 - 324
See also references of EP 3708662A4
Attorney, Agent or Firm:
HIROSE Takayuki (JP)
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