Title:
METHOD FOR PRODUCING CYCLIC PEPTIDES FROM IN VITRO PLANT CELL CULTURES
Document Type and Number:
WIPO Patent Application WO/2005/108596
Kind Code:
A1
Abstract:
The present invention relates to a method for producing cyclic peptides, polypeptides or proteins in an in vitro culture system of plant origin Violaceae species, Cucurbitaceae species or Rubiaceae species comprising the following steps: (1) cultivating a plant cell or tissue culture derived from callus, organ, embryonic, suspension or single cell cultures, in one or more nutrient media, (2) inducing expression of one or more said cyclic peptides, polypeptides or proteins, (3) recovering one or more said cyclic peptides, polypeptides or proteins from said cell or tissue culture or said medium of said cell or tissue cultures, or both and wherein the cyclic peptides, polypeptides or proteins have a cyclic cysteine knot or its chemical or structural equivalent and at least three disulfide bonds.
Inventors:
DOERNENBURG HEIKE (DE)
SEYDEL PETER (DE)
BUCHHOLZ RAINER (DE)
SEYDEL PETER (DE)
BUCHHOLZ RAINER (DE)
Application Number:
PCT/EP2005/004999
Publication Date:
November 17, 2005
Filing Date:
May 09, 2005
Export Citation:
Assignee:
UNIV FRIEDRICH ALEXANDER ER (DE)
DOERNENBURG HEIKE (DE)
SEYDEL PETER (DE)
BUCHHOLZ RAINER (DE)
DOERNENBURG HEIKE (DE)
SEYDEL PETER (DE)
BUCHHOLZ RAINER (DE)
International Classes:
C12P21/00; (IPC1-7): C12P21/04
Other References:
TRABI ET AL: "Circular proteins - no end in sight", TRENDS IN BIOCHEMICAL SCIENCES, vol. 27, 2002, pages 132 - 138, XP004343536
TRABI ET AL: "Variations in cyclotide expression in Viola species", JOURNAL OF NATURAL PRODUCTS, vol. 67, 17 April 2004 (2004-04-17), pages 806 - 810, XP018002385
SVANGÂRD ET AL: "Cytotoxic cyclotides from Viola tricolor", JOURNAL OF NATURAL PRODUCTS, vol. 67, 11 September 2003 (2003-09-11), pages 144 - 147, XP002341805
DÖRNENBURG ET AL: "Strategies for the improvement of secondary metabolite production in plant cell cultures", ENZYME AND MICROBIAL TECHNOLOGY, vol. 17, 1995, pages 674 - 684, XP002347369
ZHANG ET AL: "To stretch the boundary of secondary metabolite production in plant cell-based bioprocessing: Anthocyanin as a case study", JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY, vol. 5, December 2004 (2004-12-01), XP002341806
SEYDEL ET AL: "Poster P3 / Gewinnung von Viola tricolor-Zellinien zur Produktion zyklischer Peptide / Dechema Veranstaltungen, Frankfurt am Main, 8-9 November 2004", November 2004 (2004-11-01), pages 1 - 3, XP002347370, Retrieved from the Internet [retrieved on 20050823]
ANONYMOUS: "Institute of Bioprocess Engineering / Friedrich-Alexander-University of Erlangen-Nuremberg / Publication", February 2004 (2004-02-01), pages 1 - 4, XP002347617, Retrieved from the Internet [retrieved on 20051004]
See also references of EP 1747278A1
TRABI ET AL: "Variations in cyclotide expression in Viola species", JOURNAL OF NATURAL PRODUCTS, vol. 67, 17 April 2004 (2004-04-17), pages 806 - 810, XP018002385
SVANGÂRD ET AL: "Cytotoxic cyclotides from Viola tricolor", JOURNAL OF NATURAL PRODUCTS, vol. 67, 11 September 2003 (2003-09-11), pages 144 - 147, XP002341805
DÖRNENBURG ET AL: "Strategies for the improvement of secondary metabolite production in plant cell cultures", ENZYME AND MICROBIAL TECHNOLOGY, vol. 17, 1995, pages 674 - 684, XP002347369
ZHANG ET AL: "To stretch the boundary of secondary metabolite production in plant cell-based bioprocessing: Anthocyanin as a case study", JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY, vol. 5, December 2004 (2004-12-01), XP002341806
SEYDEL ET AL: "Poster P3 / Gewinnung von Viola tricolor-Zellinien zur Produktion zyklischer Peptide / Dechema Veranstaltungen, Frankfurt am Main, 8-9 November 2004", November 2004 (2004-11-01), pages 1 - 3, XP002347370, Retrieved from the Internet
ANONYMOUS: "Institute of Bioprocess Engineering / Friedrich-Alexander-University of Erlangen-Nuremberg / Publication", February 2004 (2004-02-01), pages 1 - 4, XP002347617, Retrieved from the Internet
See also references of EP 1747278A1
Attorney, Agent or Firm:
Scheele, Friedrich (Hinkelmann & Partner Blumenstrasse 17, München, DE)
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