Title:
METHOD FOR PRODUCING DNA-EDITED PLANT CELL, AND KIT TO BE USED THEREIN
Document Type and Number:
WIPO Patent Application WO/2021/079759
Kind Code:
A1
Abstract:
A method for producing a DNA-edited plant cell, the method comprising a step for introducing a CRISPR-Cas9 system, which contains Cas9 protein and a guide RNA thereof as constituting elements, into a plant cell, wherein the Cas9 protein is derived from Neisseria meningitidis.
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Inventors:
SAIKA HIROAKI (JP)
TOKI SEIICHI (JP)
KAYA HIDETAKA (JP)
TOKI SEIICHI (JP)
KAYA HIDETAKA (JP)
Application Number:
PCT/JP2020/038323
Publication Date:
April 29, 2021
Filing Date:
October 09, 2020
Export Citation:
Assignee:
NAT AGRICULTURE & FOOD RES ORG (JP)
International Classes:
A01H1/00; A01H5/00; C12N5/10; C12N15/09
Foreign References:
| JP2018057407A | 2018-04-12 |
Other References:
OSAKABE, KEISHI: "Establishment of highly efficient chloroplast genome editing by using synthetic custom design nucleases", GRANTS-IN-AID FOR SCIENTIFIC RESEARCH, FINAL RESEARCH REPORT, 2016, XP055819245, Retrieved from the Internet
JAGANATHAN, D. ET AL.: "CRISPR for crop improvement: An update review", FRONTIERS IN PLANT SCIENCE, vol. 9, no. Article 985, 2018, XP055647931, DOI: 10.3389/fpls.2018.00985
EDRAKI, A. ET AL.: "A compact, high-accuracy Cas9 with a dinucleotide PAM for In vivo genome editing", MOLECULAR CELL, vol. 73, no. 4, 20 December 2018 (2018-12-20), pages 714 - 726, XP055585186
JAGANATHAN, D. ET AL.: "CRISPR for crop improvement: An update review", FRONTIERS IN PLANT SCIENCE, vol. 9, no. Article 985, 2018, XP055647931, DOI: 10.3389/fpls.2018.00985
EDRAKI, A. ET AL.: "A compact, high-accuracy Cas9 with a dinucleotide PAM for In vivo genome editing", MOLECULAR CELL, vol. 73, no. 4, 20 December 2018 (2018-12-20), pages 714 - 726, XP055585186
Attorney, Agent or Firm:
CENTCREST IP ATTORNEYS (JP)
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