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Title:
METHOD FOR PRODUCING DNA FRAGMENTS HAVING DESIRED BASE SEQUENCE
Document Type and Number:
WIPO Patent Application WO/2018/147070
Kind Code:
A1
Abstract:
The present invention provides a method whereby at least two DNA materials are assembled and DNA fragments having a desired base sequence are produced. This method comprises: a step (1) in which at least two DNA materials are linked and a precursor DNA fragment is obtained that has part of a desired base sequence and a linking base sequence alternating therein; and a step (2) in which the precursor DNA fragment is treated using a restriction enzyme, the linking base sequences are removed, and a DNA fragment having the desired base sequence is obtained. The base sequences recognized by the restriction enzyme are arranged in the precursor DNA fragment such that, when the base sequences are treated using the restriction enzyme and the precursor DNA fragment is cleaved, the linking base sequences are removed and the parts of the desired base sequences that are adjacent via the linking base sequences can be linked at the cleaved sites.

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Inventors:
YACHIE NOZOMU (JP)
ADACHI DAISUKE (JP)
SATO CHIKAKO (JP)
Application Number:
PCT/JP2018/001954
Publication Date:
August 16, 2018
Filing Date:
January 23, 2018
Export Citation:
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Assignee:
SPIBER INC (JP)
International Classes:
C12N15/09; C12N15/11
Foreign References:
JP2002236125A2002-08-23
US20100291633A12010-11-18
US7723077B22010-05-25
JP2011512140A2011-04-21
Other References:
ENGLER, C. ET AL.: "Golden gate shuffling: a one-pot DNA shuffling method based on type IIs restriction enzymes", PLOS ONE, vol. 4, no. 5, 2009, pages e5554, XP055533834
TSUGE, KENJI ET AL.: "Establishment of gene cluster unit for re-editing genome", BIOTECHNOLOGY, AND AGROCHEMISTRY, vol. 54, no. 10, 2016, pages 740 - 746
Attorney, Agent or Firm:
HASEGAWA Yoshiki et al. (JP)
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