Title:
METHOD FOR PRODUCING DOPAMINERGIC NEURONS
Document Type and Number:
WIPO Patent Application WO/2015/020234
Kind Code:
A1
Abstract:
The present invention provides a method for more efficiently producing high-quality dopaminergic neurons from neuron precursor cells. More specifically, the present invention provides a method for producing dopaminergic neurons, which is characterized by subjecting neuron precursor cells to a step for culturing using a culture medium comprising (i) a cAMP analog and (ii) an MEK inhibitor. Furthermore, the present invention also provides a drug containing the dopaminergic neurons obtained by said method, as well as a reagent and kit for use with said method.
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Inventors:
SHOJI MASANOBU (JP)
Application Number:
PCT/JP2014/071352
Publication Date:
February 12, 2015
Filing Date:
August 06, 2014
Export Citation:
Assignee:
TAKEDA PHARMACEUTICAL (JP)
International Classes:
C12N5/0793; A61K35/12; A61L27/00; A61P25/16
Domestic Patent References:
| WO2004081172A2 | 2004-09-23 | |||
| WO2013067362A1 | 2013-05-10 |
Foreign References:
| US20120094381A1 | 2012-04-19 | |||
| JP2013163062A | 2013-08-22 |
Other References:
HWANG D.Y. ET AL.: "Human ES and iPS cells as cell sources for the treatment of Parkinson's disease: current state and problems", J. CELL . BIOCHEM., vol. 109, no. 2, pages 292 - 301, XP055314361
KRIKS S. ET AL.: "Dopamine neurons derived from human ES cells efficiently engraft in animal models of Parkinson's disease", NATURE, vol. 480, no. 7378, pages 547 - 551, XP055271235
JAEGER I. ET AL.: "Temporally controlled modulation of FGF/ERK signaling directs midbrain dopaminergic neural progenitor fate in mouse and human pluripotent stem cells", DEVELOPMENT, vol. 138, no. 20, October 2011 (2011-10-01), pages 4363 - 4374, XP055314360
DENHAM M. ET AL.: "Glycogen synthase kinase 3beta and activin/nodal inhibition in human embryonic stem cells induces a pre-neuroepithelial state that is required for specification to a floor plate cell lineage", STEM CELLS, vol. 30, no. 11, November 2012 (2012-11-01), pages 2400 - 2411, XP055314359
KUPERSHMIDT L. ET AL.: "The neuroprotective effect of Activin A and B: implication for neurodegenerative diseases", J. NEUROCHEM., vol. 103, no. 3, November 2007 (2007-11-01), pages 962 - 971, XP055139439
SUZUKI K. ET AL.: "Activin A induces neuronal differentiation and survival via ALK4 in a SMAD-independent manner in a subpopulation of human neuroblastomas", BIOCHEM. BIOPHYS. RES. COMMUN., vol. 394, no. 3, 9 April 2010 (2010-04-09), pages 639 - 645, XP055314358
FASANO ET AL., CELL STEM CELL, vol. 6, 2010, pages 336 - 347
KRIKS ET AL., NATURE, vol. 480, 2011, pages 547 - 553
KIRKEBY ET AL., CELL REPORTS, vol. 1, 2012, pages 703 - 714
XI ET AL., STEM CELLS, vol. 30, 2012, pages 1655 - 1663
DENHAM ET AL., STEM CELLS, vol. 30, 2012, pages 2400 - 2411
EG CELL: PROC NATL ACAD SCI U S A., vol. 95, 1998, pages 13726 - 31
GS CELL: NATURE, vol. 456, 2008, pages 344 - 9
EVANS ET AL., NATURE, vol. 292, 1981, pages 154 - 6
MARTIN GR ET AL., PROC NATL ACAD SCI, vol. 78, 1981, pages 7634 - 8
THOMSON ET AL., SCIENCE, vol. 282, 1998, pages 1145 - 7
NAT BIOTECHNOL, vol. 26, 2008, pages 101 - 106
NATURE, vol. 454, no. 7204, 31 July 2008 (2008-07-31), pages 646 - 50
CELL STEM CELL, vol. 4, no. 1, 9 January 2009 (2009-01-09), pages 16 - 9
CELL STEM CELL., vol. 5, no. 5, 6 November 2009 (2009-11-06), pages 491 - 503
CELL STEM CELL., vol. 4, no. 5, 8 May 2009 (2009-05-08), pages 381 - 4
BREWER G.J. ET AL., J. NEUROSCI. RES., vol. 35, 1993, pages 567
LING, N. ET AL., NATURE, vol. 321, 1986, pages 779 - 782
VALE, W. ET AL., NATURE, vol. 321, 1986, pages 776 - 779
NATURE NEUROSCIENCE, vol. 2, 1999, pages 1137
N ENGL J MED., vol. 344, 2001, pages 710 - 9
NATURE BIOTECHNOLOGY, vol. 26, 2008, pages 101 - 106
CELL., vol. 131, 2007, pages 861 - 872
See also references of EP 3031908A4
KRIKS S. ET AL.: "Dopamine neurons derived from human ES cells efficiently engraft in animal models of Parkinson's disease", NATURE, vol. 480, no. 7378, pages 547 - 551, XP055271235
JAEGER I. ET AL.: "Temporally controlled modulation of FGF/ERK signaling directs midbrain dopaminergic neural progenitor fate in mouse and human pluripotent stem cells", DEVELOPMENT, vol. 138, no. 20, October 2011 (2011-10-01), pages 4363 - 4374, XP055314360
DENHAM M. ET AL.: "Glycogen synthase kinase 3beta and activin/nodal inhibition in human embryonic stem cells induces a pre-neuroepithelial state that is required for specification to a floor plate cell lineage", STEM CELLS, vol. 30, no. 11, November 2012 (2012-11-01), pages 2400 - 2411, XP055314359
KUPERSHMIDT L. ET AL.: "The neuroprotective effect of Activin A and B: implication for neurodegenerative diseases", J. NEUROCHEM., vol. 103, no. 3, November 2007 (2007-11-01), pages 962 - 971, XP055139439
SUZUKI K. ET AL.: "Activin A induces neuronal differentiation and survival via ALK4 in a SMAD-independent manner in a subpopulation of human neuroblastomas", BIOCHEM. BIOPHYS. RES. COMMUN., vol. 394, no. 3, 9 April 2010 (2010-04-09), pages 639 - 645, XP055314358
FASANO ET AL., CELL STEM CELL, vol. 6, 2010, pages 336 - 347
KRIKS ET AL., NATURE, vol. 480, 2011, pages 547 - 553
KIRKEBY ET AL., CELL REPORTS, vol. 1, 2012, pages 703 - 714
XI ET AL., STEM CELLS, vol. 30, 2012, pages 1655 - 1663
DENHAM ET AL., STEM CELLS, vol. 30, 2012, pages 2400 - 2411
EG CELL: PROC NATL ACAD SCI U S A., vol. 95, 1998, pages 13726 - 31
GS CELL: NATURE, vol. 456, 2008, pages 344 - 9
EVANS ET AL., NATURE, vol. 292, 1981, pages 154 - 6
MARTIN GR ET AL., PROC NATL ACAD SCI, vol. 78, 1981, pages 7634 - 8
THOMSON ET AL., SCIENCE, vol. 282, 1998, pages 1145 - 7
NAT BIOTECHNOL, vol. 26, 2008, pages 101 - 106
NATURE, vol. 454, no. 7204, 31 July 2008 (2008-07-31), pages 646 - 50
CELL STEM CELL, vol. 4, no. 1, 9 January 2009 (2009-01-09), pages 16 - 9
CELL STEM CELL., vol. 5, no. 5, 6 November 2009 (2009-11-06), pages 491 - 503
CELL STEM CELL., vol. 4, no. 5, 8 May 2009 (2009-05-08), pages 381 - 4
BREWER G.J. ET AL., J. NEUROSCI. RES., vol. 35, 1993, pages 567
LING, N. ET AL., NATURE, vol. 321, 1986, pages 779 - 782
VALE, W. ET AL., NATURE, vol. 321, 1986, pages 776 - 779
NATURE NEUROSCIENCE, vol. 2, 1999, pages 1137
N ENGL J MED., vol. 344, 2001, pages 710 - 9
NATURE BIOTECHNOLOGY, vol. 26, 2008, pages 101 - 106
CELL., vol. 131, 2007, pages 861 - 872
See also references of EP 3031908A4
Attorney, Agent or Firm:
TAKASHIMA, HAJIME (JP)
Takashima 1 (JP)
Takashima 1 (JP)
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