Title:
METHOD FOR PRODUCING DOPAMINERGIC NEURONS
Document Type and Number:
WIPO Patent Application WO/2018/193949
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a method for efficiently producing dopaminergic neurons within a short period of time. Dopaminergic neurons are produced by a method comprising: (1) a step for culturing pluripotent stem cells in the presence of a TGF-β family inhibitor, a GSK3β inhibitor and a BMP inhibitor; (2) a step for suspension-culturing the cells obtained in step (1) in the presence of a TGF-β family inhibitor, a GSK3β inhibitor, FGF8 and a hedgehog signal agonist under normal oxygen partial pressure so as to form neurospheres; and (3) a step for collecting cells constituting the neurospheres and inducing the differentiation thereof into dopaminergic neurons.
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Inventors:
OZAKI NORIO (JP)
ARIOKA YUKO (JP)
MORI DAISUKE (JP)
KUSHIMA ITARU (JP)
ARIOKA YUKO (JP)
MORI DAISUKE (JP)
KUSHIMA ITARU (JP)
Application Number:
PCT/JP2018/015304
Publication Date:
October 25, 2018
Filing Date:
April 11, 2018
Export Citation:
Assignee:
UNIV NAGOYA NAT UNIV CORP (JP)
International Classes:
C12N5/0793; C12N1/00; C12N5/10
Domestic Patent References:
| WO2017009766A1 | 2017-01-19 | |||
| WO2016115407A1 | 2016-07-21 | |||
| WO2013187416A1 | 2013-12-19 | |||
| WO2015020234A1 | 2015-02-12 | |||
| WO1998030679A1 | 1998-07-16 | |||
| WO2005123902A1 | 2005-12-29 |
Foreign References:
| US20150010515A1 | 2015-01-08 | |||
| JP2017082600A | 2017-05-18 |
Other References:
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FUJIMORI KOKI ET AL.: "Modeling neurological diseases with induced pluripotent cells reprogrammed from immortalized lymphoblastoid cell lines", MOLECULAR BRAIN, vol. 9, no. 1, 3 October 2016 (2016-10-03), pages 88, XP055560521, Retrieved from the Internet
CHEN HM ET AL.: "Transcripts involved in calcium signaling and telencephalic neuronal fate are altered in induced pluripotent stem cells from bipolar disorder patients", TRANSLATIONAL PSYCHIATRY, vol. 4, 25 March 2014 (2014-03-25), pages e375, XP055560524, Retrieved from the Internet
See also references of EP 3613848A4
STEM CELL REPORTS, vol. 6, no. 3, 8 March 2016 (2016-03-08), pages 422 - 35
MOLECULAR BRAIN, vol. 9, 2016, pages 88
PLOS ONE, vol. 9, no. 2, 21 February 2014 (2014-02-21), pages e87388
BIOCHIM BIOPHYS ACTA, vol. 1850, no. 1, September 2015 (2015-09-01), pages 1669 - 32
NAT COMMUN., vol. 7, 14 October 2016 (2016-10-14), pages 13097
"Manipulating the Mouse Embryo A Laboratory Manual", 1994, COLD SPRING HARBOR LABORATORY PRESS
THOMSON, JA ET AL., SCIENCE, vol. 282, 1998, pages 1145 - 1147
WILMUT ET AL., NATURE, vol. 385, 1997, pages 810
CIBELLI ET AL., SCIENCE, vol. 318, no. 5858, 2007, pages 1917 - 1920
WAKAYAMA ET AL., NATURE, vol. 394, 1998, pages 369
AKIRA IRITANI ET AL., TANPAKUSHITSU KAKUSAN KOSO, vol. 44, 1999, pages 892
BAGUISI ET AL., NATURE BIOTECHNOLOGY, vol. 17, 1999, pages 456
NATURE GENETICS, vol. 22, 1999, pages 127
PROC. NATL. ACAD. SCI. USA, vol. 96, 1999, pages 14984
RIDEOUT III ET AL., NATURE GENETICS, vol. 24, 2000, pages 109
TACHIBANA ET AL.: "Human Embryonic Stem Cells Derived by Somatic Cell Nuclear Transfer", CELL, 2013
NAKAJIMA ET AL., STEM CELLS, vol. 25, 2007, pages 983 - 985
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MATSUI ET AL., CELL, vol. 70, 1992, pages 841 - 847
SHAMBLOTT ET AL., PROC. NATL. ACAD. SCI. USA, vol. 95, no. 23, 1998, pages 13726 - 13731
TURNPENNY ET AL., STEM CELLS, vol. 21, no. 5, 2003, pages 598 - 609
TAKAHASHI KYAMANAKA S, CELL, vol. 126, no. 4, 2006, pages 663 - 676
TAKAHASHI, K ET AL., CELL, vol. 131, no. 5, 2007, pages 861 - 72
NAKAGAWA M ET AL., NAT. BIOTECHNOL., vol. 26, no. 11, 2008, pages 1269 - 1275
KIM JB ET AL., NATURE, vol. 454, no. 7204, 2008, pages 646 - 650
KIM JB ET AL., CELL, vol. 136, no. 3, 2009, pages 411 - 419
KIM DKIM CHMOON JI ET AL., CELL STEM CELL, vol. 4, 2009, pages 472 - 476
SILVA J ET AL., PLOS. BIOL., vol. 6, no. 10, 2008, pages e 253
STADTFELD M ET AL., SCIENCE, vol. 322, no. 5903, 2008, pages 949 - 953
WOLTJEN KMICHAEL IPMOHSENI P ET AL., NATURE, vol. 458, 2009, pages 771 - 775
YUSA KRAD RTAKEDA J ET AL., NAT METHODS, vol. 6, 2009, pages 363 - 369
YU JHU KSMUGA-OTTO KTIAN S ET AL., SCIENCE, vol. 324, 2009, pages 797 - 801
WATANABE ET AL., NATURE NEUROSCIENCE, vol. 8, 2005, pages 288 - 296
REYNOLDS BAWEISS S., SCIENCE, USA, vol. 255, no. 5052, 27 March 1992 (1992-03-27), pages 1707 - 10
OKITA, K. ET AL.: "A more efficient method to generate integration-free human iPS cells", NATURE METHODS, vol. 8, 2011, pages 409 - 412, XP055176852, DOI: 10.1038/nmeth.1591
FUJIMORI, K. ET AL.: "Modeling neurological diseases with induced pluripotent cells reprogrammed from immortalized lymphoblastoid cell lines", MOLECULAR BRAIN, vol. 9, 2016, pages 88, XP055560521, DOI: 10.1186/s13041-016-0267-6
HOOK, V. ET AL.: "Human iPSC neurons display activity-dependent neurotransmitter secretion: aberrant catecholamine levels in schizophrenia neurons", STEM CELL REPORTS, vol. 3, 2014, pages 531 - 538
ROBICSEK, O. ET AL.: "Abnormal neuronal differentiation and mitochondrial dysfunction in hair follicle-derived induced pluripotent stem cells of schizophrenia patients", MOLECULAR PSYCHIATRY, vol. 18, 2013, pages 1067 - 1076
BODEA, G.O. ET AL., REELIN AND CXCL12 REGULATE DISTINCT MIGRATORY BEHAVIORS DURING THE DEVELOPMENT OF THE DOPAMINERGIC SYSTEM., ENGLAND, vol. 141, 2014, pages 661 - 673
FUJIMORI KOKI ET AL.: "Modeling neurological diseases with induced pluripotent cells reprogrammed from immortalized lymphoblastoid cell lines", MOLECULAR BRAIN, vol. 9, no. 1, 3 October 2016 (2016-10-03), pages 88, XP055560521, Retrieved from the Internet
CHEN HM ET AL.: "Transcripts involved in calcium signaling and telencephalic neuronal fate are altered in induced pluripotent stem cells from bipolar disorder patients", TRANSLATIONAL PSYCHIATRY, vol. 4, 25 March 2014 (2014-03-25), pages e375, XP055560524, Retrieved from the Internet
See also references of EP 3613848A4
STEM CELL REPORTS, vol. 6, no. 3, 8 March 2016 (2016-03-08), pages 422 - 35
MOLECULAR BRAIN, vol. 9, 2016, pages 88
PLOS ONE, vol. 9, no. 2, 21 February 2014 (2014-02-21), pages e87388
BIOCHIM BIOPHYS ACTA, vol. 1850, no. 1, September 2015 (2015-09-01), pages 1669 - 32
NAT COMMUN., vol. 7, 14 October 2016 (2016-10-14), pages 13097
"Manipulating the Mouse Embryo A Laboratory Manual", 1994, COLD SPRING HARBOR LABORATORY PRESS
THOMSON, JA ET AL., SCIENCE, vol. 282, 1998, pages 1145 - 1147
WILMUT ET AL., NATURE, vol. 385, 1997, pages 810
CIBELLI ET AL., SCIENCE, vol. 318, no. 5858, 2007, pages 1917 - 1920
WAKAYAMA ET AL., NATURE, vol. 394, 1998, pages 369
AKIRA IRITANI ET AL., TANPAKUSHITSU KAKUSAN KOSO, vol. 44, 1999, pages 892
BAGUISI ET AL., NATURE BIOTECHNOLOGY, vol. 17, 1999, pages 456
NATURE GENETICS, vol. 22, 1999, pages 127
PROC. NATL. ACAD. SCI. USA, vol. 96, 1999, pages 14984
RIDEOUT III ET AL., NATURE GENETICS, vol. 24, 2000, pages 109
TACHIBANA ET AL.: "Human Embryonic Stem Cells Derived by Somatic Cell Nuclear Transfer", CELL, 2013
NAKAJIMA ET AL., STEM CELLS, vol. 25, 2007, pages 983 - 985
KIM ET AL., CELL STEM CELL, vol. 1, 2007, pages 346 - 352
REVAZOVA ET AL., CLONING STEM CELLS, vol. 9, 2007, pages 432 - 449
REVAZOVA ET AL., CLONING STEM CELLS, vol. 10, 2008, pages 11 - 24
STREGKCHENKO N. ET AL., REPROD BIOMED ONLINE, vol. 9, 2004, pages 623 - 629
KLIMANSKAYA I. ET AL., NATURE, vol. 444, 2006, pages 481 - 485
CHUNG Y. ET AL., CELL STEM CELL, vol. 2, 2008, pages 113 - 117
ZHANG X. ET AL., STEM CELLS, vol. 24, 2006, pages 2669 - 2676
WASSARMAN, P.M. ET AL., METHODS IN ENZYMOLOGY, vol. 365, 2003
MATSUI ET AL., CELL, vol. 70, 1992, pages 841 - 847
SHAMBLOTT ET AL., PROC. NATL. ACAD. SCI. USA, vol. 95, no. 23, 1998, pages 13726 - 13731
TURNPENNY ET AL., STEM CELLS, vol. 21, no. 5, 2003, pages 598 - 609
TAKAHASHI KYAMANAKA S, CELL, vol. 126, no. 4, 2006, pages 663 - 676
TAKAHASHI, K ET AL., CELL, vol. 131, no. 5, 2007, pages 861 - 72
NAKAGAWA M ET AL., NAT. BIOTECHNOL., vol. 26, no. 11, 2008, pages 1269 - 1275
KIM JB ET AL., NATURE, vol. 454, no. 7204, 2008, pages 646 - 650
KIM JB ET AL., CELL, vol. 136, no. 3, 2009, pages 411 - 419
KIM DKIM CHMOON JI ET AL., CELL STEM CELL, vol. 4, 2009, pages 472 - 476
SILVA J ET AL., PLOS. BIOL., vol. 6, no. 10, 2008, pages e 253
STADTFELD M ET AL., SCIENCE, vol. 322, no. 5903, 2008, pages 949 - 953
WOLTJEN KMICHAEL IPMOHSENI P ET AL., NATURE, vol. 458, 2009, pages 771 - 775
YUSA KRAD RTAKEDA J ET AL., NAT METHODS, vol. 6, 2009, pages 363 - 369
YU JHU KSMUGA-OTTO KTIAN S ET AL., SCIENCE, vol. 324, 2009, pages 797 - 801
WATANABE ET AL., NATURE NEUROSCIENCE, vol. 8, 2005, pages 288 - 296
REYNOLDS BAWEISS S., SCIENCE, USA, vol. 255, no. 5052, 27 March 1992 (1992-03-27), pages 1707 - 10
OKITA, K. ET AL.: "A more efficient method to generate integration-free human iPS cells", NATURE METHODS, vol. 8, 2011, pages 409 - 412, XP055176852, DOI: 10.1038/nmeth.1591
FUJIMORI, K. ET AL.: "Modeling neurological diseases with induced pluripotent cells reprogrammed from immortalized lymphoblastoid cell lines", MOLECULAR BRAIN, vol. 9, 2016, pages 88, XP055560521, DOI: 10.1186/s13041-016-0267-6
HOOK, V. ET AL.: "Human iPSC neurons display activity-dependent neurotransmitter secretion: aberrant catecholamine levels in schizophrenia neurons", STEM CELL REPORTS, vol. 3, 2014, pages 531 - 538
ROBICSEK, O. ET AL.: "Abnormal neuronal differentiation and mitochondrial dysfunction in hair follicle-derived induced pluripotent stem cells of schizophrenia patients", MOLECULAR PSYCHIATRY, vol. 18, 2013, pages 1067 - 1076
BODEA, G.O. ET AL., REELIN AND CXCL12 REGULATE DISTINCT MIGRATORY BEHAVIORS DURING THE DEVELOPMENT OF THE DOPAMINERGIC SYSTEM., ENGLAND, vol. 141, 2014, pages 661 - 673
Attorney, Agent or Firm:
HAGINO Mikiharu (JP)
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