METHOD FOR PRODUCING ENZYMATIC REACTION BY USING ADENOSINE TO REPLACE ATP

WIPO Patent Application WO/2018/228247

A1

Disclosed in the present application are a method for producing an enzymatic reaction by using adenosine to replace adenosine triphosphate (ATP) and an application thereof. The method comprises the following steps: (1) in an enzymatic reaction system, adding an ATP regenerating enzyme, adenylate kinase (AK) enzyme and adenosine in proportion to carry out an enzymatic reaction; (2) directly separating an immobilized ATP regenerating enzyme and AK enzyme in a reaction tank, and separating a free ATP regenerating enzyme and AK enzyme by means of an ultrafiltration membrane in a filter; (3) separating and purifying the filtrate obtained in step (2) to obtain a product. The present invention has the following beneficial effects: 1) Using adenosine to replace ATP or adenosine monophosphate (AMP) saves a large amount of costs in industrial production; 2) a stable enzyme recovery system is established, thus saving energy and being environmentally friendly; 3) a small amount of generated by-product ATP, adenosine diphosphate (ADP) and AMP may be directly used in a cyclic reaction, or used to produce ATP, or concentrated and purified by means of filtration, ion exchange and like methods, while operation is simple.

LIU SHANSHAN (CN)
LIU HUI (CN)
ZHOU WENWEN (CN)
QIN YONGFA (CN)

PCT/CN2018/090050

December 20, 2018

June 06, 2018

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ANHUI GSH BIO TECH CO LTD (CN)

C12P19/32; C12N9/12; C12P13/00; C12P13/06; C12P13/14; C12P19/30; C12P21/02

CN106191170A	2016-12-07
CN105219823A	2016-01-06
CN105603028A	2016-05-25
CN105861598A	2016-08-17
CN102978267A	2013-03-20
CN105647996A	2016-06-08

CHINA KINTEC IP LAWS LLP (CN)

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