Title:
METHOD FOR PRODUCING ETHANOL USING RECOMBINANT YEAST
Document Type and Number:
WIPO Patent Application WO/2014/021163
Kind Code:
A1
Abstract:
The purpose of the present invention is to improve the efficiency of ethanol fermentation in the presence of acetic acid and to achieve excellent ethanol productivity.
A yeast mutant strain, which has a deficiency of an acetoaldehyde dehydrogenase gene corresponding to at least one acetoaldehyde dehydrogenase gene selected from the group consisting of ALD4 gene, ALD5 gene and ALD6 gene in Saccharomyces cerevisiae, is cultured in a culture medium containing acetic acid, and ethanol in the culture medium is then collected.
Inventors:
ONISHI TORU (JP)
Application Number:
PCT/JP2013/070036
Publication Date:
February 06, 2014
Filing Date:
July 24, 2013
Export Citation:
Assignee:
TOYOTA MOTOR CO LTD (JP)
ONISHI TORU (JP)
ONISHI TORU (JP)
International Classes:
C12P7/06; C12N15/09
Domestic Patent References:
| WO2011065539A1 | 2011-06-03 |
Foreign References:
| JP2010239925A | 2010-10-28 | |||
| US20120122172A1 | 2012-05-17 |
Other References:
SONDEREGGER, M ET AL.: "Metabolic engineering of a phosphoketolase pathway for pentose catabolism in Saccharomyces cerevisiae.", APPL. ENVIRON. MICROBIOL., vol. 70, no. 5, 2004, pages 2892 - 2897
EGLINTON, J. M. ET AL.: "Decreasing acetic acid accumulation by a glycerol overproducing strain of Saccharomyces cerevisiae by deleting the ALD6 aldehyde dehydrogenase gene.", YEAST, vol. 19, 2002, pages 295 - 301
WANG, X. ET AL.: "Molecular cloning, characterization, and potential roles of cytosolic and mitochondrial aldehyde dehydrogenases in ethanol metabolism in Saccharomyces cerevisiae.", J. BACTERIOL., vol. 180, no. 4, 1998, pages 822 - 830
REMIZE, F ET AL.: "Engineering of the pyruvate dehydrogenase bypass in Saccharomyces cerevisiae: role of the cytosolic Mg2+ and mitochondrial K+ acetaldehyde dehydrogenases Ald6p and Ald4p in acetate formation during alcoholic fermentation.", APPL. ENVIRON. MICROBIOL., vol. 66, no. 8, 2000, pages 3151 - 3159
SAINT-PRIX, F. ET AL.: "Functional analysis of the ALD gene family of Saccharomyces cerevisiae during anaerobic growth on glucose: the NADP'-dependent Ald6p and Ald5p isoforms play a major role in acetate formation.", MICROBIOLOGY, vol. 150, 2004, pages 2209 - 2220
EGLINTON, J. M. ET AL.: "Decreasing acetic acid accumulation by a glycerol overproducing strain of Saccharomyces cerevisiae by deleting the ALD6 aldehyde dehydrogenase gene.", YEAST, vol. 19, 2002, pages 295 - 301
WANG, X. ET AL.: "Molecular cloning, characterization, and potential roles of cytosolic and mitochondrial aldehyde dehydrogenases in ethanol metabolism in Saccharomyces cerevisiae.", J. BACTERIOL., vol. 180, no. 4, 1998, pages 822 - 830
REMIZE, F ET AL.: "Engineering of the pyruvate dehydrogenase bypass in Saccharomyces cerevisiae: role of the cytosolic Mg2+ and mitochondrial K+ acetaldehyde dehydrogenases Ald6p and Ald4p in acetate formation during alcoholic fermentation.", APPL. ENVIRON. MICROBIOL., vol. 66, no. 8, 2000, pages 3151 - 3159
SAINT-PRIX, F. ET AL.: "Functional analysis of the ALD gene family of Saccharomyces cerevisiae during anaerobic growth on glucose: the NADP'-dependent Ald6p and Ald5p isoforms play a major role in acetate formation.", MICROBIOLOGY, vol. 150, 2004, pages 2209 - 2220
Attorney, Agent or Firm:
HIRAKI Yusuke et al. (JP)
Yusuke Hiraki (JP)
Yusuke Hiraki (JP)
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