Title:
METHOD FOR PRODUCING EXOSOMES AND EXOSOMES OBTAINED THEREBY
Document Type and Number:
WIPO Patent Application WO/2019/142853
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a method for causing exosomes to contain an arbitrary protein, lipid, carbohydrate, or nucleic acid. The above problem can be solved by a method for producing exosomes that includes (a) a step for adding a biological toxin having perforation activity to a medium that contains cells and incubating, (b) a step for adding ATP and incubating, and (c) a step for adding a medium that contains a calcium ion and incubating.
Inventors:
KANO FUMI (JP)
MURATA MASAYUKI (JP)
SONODA YUKI (JP)
MURATA MASAYUKI (JP)
SONODA YUKI (JP)
Application Number:
PCT/JP2019/001230
Publication Date:
July 25, 2019
Filing Date:
January 17, 2019
Export Citation:
Assignee:
TOKYO INST TECH (JP)
International Classes:
C12N5/07; A61K51/02
Domestic Patent References:
| WO2016178532A1 | 2016-11-10 | |||
| WO2016076347A1 | 2016-05-19 |
Foreign References:
| JP2014185090A | 2014-10-02 | |||
| JP2017521644A | 2017-08-03 | |||
| JP2011120520A | 2011-06-23 | |||
| JP2010285426A | 2010-12-24 | |||
| JP2017038566A | 2017-02-23 | |||
| JP2013213688A | 2013-10-17 |
Other References:
ROMERO, MATTHEW ET AL.: "Intrinsic repair protects cells from pore-forming toxins by microvesicle shedding", CELL DEATH AND DIFFERENTIATION, vol. 24, no. 5, 2017, pages 798 - 808, XP055628620
MASAYUKI MURATA ET AL: "Semi-intact Cell System and Cell Resealing Technique for Image-based Analysis of Intracellular Events: A Powerful Cellular Tool for Single Cell Biology", SEITBUTSU BUTSURI, vol. 54, no. 4, 2014, pages 206 - 209, XP055628627
ABE ET AL: "26U-am10S . Abstract Construction of quantitative evaluation system of microvesicles derived from a living body by flow cytometry", THE 135TH ANNUAL MEETING OF THE PHARMACEUTICAL SOCIETY OF JAPAN, 2015, pages 63
YIM, NAMBIN ET AL.: "Exosome engineering for effficient intracellular delivery of soluble proteins using optically reversible protein-protein interaction module", NATURE COMMUNICATIONS, vol. 7, no. 12277, 2016, pages 1 - 9, XP002779472
STICKNEY, ZACHARY ET AL.: "Development of exosome surface display technology in living human cells", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMNICATIONS, vol. 472, 2016, pages 53 - 59, XP029445487, DOI: 10.1016/j.bbrc.2016.02.058
MASAYUKI MURATA ET AL: "Semi-intact Cell System and Cell Resealing Technique for Image-based Analysis of Intracellular Events: A Powerful Cellular Tool for Single Cell Biology", SEITBUTSU BUTSURI, vol. 54, no. 4, 2014, pages 206 - 209, XP055628627
ABE ET AL: "26U-am10S . Abstract Construction of quantitative evaluation system of microvesicles derived from a living body by flow cytometry", THE 135TH ANNUAL MEETING OF THE PHARMACEUTICAL SOCIETY OF JAPAN, 2015, pages 63
YIM, NAMBIN ET AL.: "Exosome engineering for effficient intracellular delivery of soluble proteins using optically reversible protein-protein interaction module", NATURE COMMUNICATIONS, vol. 7, no. 12277, 2016, pages 1 - 9, XP002779472
STICKNEY, ZACHARY ET AL.: "Development of exosome surface display technology in living human cells", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMNICATIONS, vol. 472, 2016, pages 53 - 59, XP029445487, DOI: 10.1016/j.bbrc.2016.02.058
Attorney, Agent or Firm:
YAMAGUCHI Kenjiro et al. (JP)
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