Title:
A METHOD OF PRODUCING A FUNCTIONAL IMMUNOGLOBULIN SUPERFAMILY PROTEIN
Document Type and Number:
WIPO Patent Application WO2000015665
Kind Code:
A3
Abstract:
The present invention relates to a process of producing a functional immunoglobulin superfamily protein, which has at least one disulphide bond when functional, the process comprising the steps of providing a bacterial cell, where the gene is expressible, isolating the protein from the cell without reducing it and subjecting the isolated protein to a folding treatment. Preferably, the immunoglobulin superfamily protein is selected from the group consisting of Class I and Class II histocompatibility molecules and beta 2 microglobulin (beta2m). Other embodiments of the invention is a stable peptide free MHC protein obtainable by a process of the invention and a kit comprising a MHC class I heavy chain and a abeta2m allowing the recipient to produce and measure or detect a functional MHC class I protein to which a peptide, which is capabe of binding to said MHC class I protein, can be added leading to the generation of a functional MHC class I protein.
Inventors:
PEDERSEN LARS OESTERGAARD (DK)
BUUS SOEREN (DK)
BUUS SOEREN (DK)
Application Number:
PCT/DK1999/000484
Publication Date:
August 17, 2000
Filing Date:
September 14, 1999
Export Citation:
Assignee:
PEDERSEN LARS OESTERGAARD (DK)
BUUS SOEREN (DK)
BUUS SOEREN (DK)
International Classes:
G01N33/68; A61K38/00; A61P35/00; A61P37/02; C07K14/74; C12N15/09; C12P21/02; G01N30/88; C12R1/19; (IPC1-7): C07K14/705; C12P21/02; G01N33/68
Domestic Patent References:
| WO1995011702A1 | 1995-05-04 | |||
| WO1997044667A2 | 1997-11-27 | |||
| WO1993022332A2 | 1993-11-11 | |||
| WO1994006813A1 | 1994-03-31 |
Foreign References:
| US5529921A | 1996-06-25 |
Other References:
GARBOCZI D N ET AL: "HLA-A2-peptide complexes: refolding and crystallization of molecules expressed in Escherichia coli and complexed with single antigenic peptides.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, (1992 APR 15) 89 (8) 3429-33., XP002131059
STEVENS JAMES ET AL: "Efficient generation of major histocompatibility complex class I-peptide complexes using synthetic peptide libraries.", JOURNAL OF BIOLOGICAL CHEMISTRY JAN. 30, 1998, vol. 273, no. 5, 30 January 1998 (1998-01-30), pages 2874 - 2884, XP002136475, ISSN: 0021-9258
PARKER K C ET AL: "An HLA-A2/beta 2-microglobulin/peptide complex assembled from subunits expressed separately in Escherichia coli.", MOLECULAR IMMUNOLOGY, (1992 MAR) 29 (3) 371-8., XP000876800
PLAKSIN D ET AL: "A T cell receptor V alpha domain expressed in bacteria: does it dimerize in solution?.", JOURNAL OF EXPERIMENTAL MEDICINE, (1996 OCT 1) 184 (4) 1251-8., XP000876791
DATABASE MEDLINE [online] US NATIONAL LIBRARY OF MEDICINE (NLM), BETHESDA, MD, US; MOLLOY P E ET AL: "Production of soluble single-chain T-cell receptor fragments in Escherichia coli trxB mutants.", XP002131051, retrieved from STN Database accession no. 1998346783
STEVENS JAMES ET AL: "Efficient generation of major histocompatibility complex class I-peptide complexes using synthetic peptide libraries.", JOURNAL OF BIOLOGICAL CHEMISTRY JAN. 30, 1998, vol. 273, no. 5, 30 January 1998 (1998-01-30), pages 2874 - 2884, XP002136475, ISSN: 0021-9258
PARKER K C ET AL: "An HLA-A2/beta 2-microglobulin/peptide complex assembled from subunits expressed separately in Escherichia coli.", MOLECULAR IMMUNOLOGY, (1992 MAR) 29 (3) 371-8., XP000876800
PLAKSIN D ET AL: "A T cell receptor V alpha domain expressed in bacteria: does it dimerize in solution?.", JOURNAL OF EXPERIMENTAL MEDICINE, (1996 OCT 1) 184 (4) 1251-8., XP000876791
DATABASE MEDLINE [online] US NATIONAL LIBRARY OF MEDICINE (NLM), BETHESDA, MD, US; MOLLOY P E ET AL: "Production of soluble single-chain T-cell receptor fragments in Escherichia coli trxB mutants.", XP002131051, retrieved from STN Database accession no. 1998346783
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