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Patent Searching and Data


Title:
METHOD FOR PRODUCING GENE KNOCK-IN CELLS
Document Type and Number:
WIPO Patent Application WO/2018/030208
Kind Code:
A1
Abstract:
The present inventor attempted to produce a knock-in animal with the use of a single-stranded DNA as a donor by utilizing a genome editing system comprising a nuclease in the form of a protein and a DNA-targeting RNA. As a result, it was found that cells wherein the donor DNA was knocked-in could be thus obtained at an extremely high efficiency.

Inventors:
AIDA TOMOMI (JP)
TANAKA KOHICHI (JP)
Application Number:
PCT/JP2017/027838
Publication Date:
February 15, 2018
Filing Date:
August 01, 2017
Export Citation:
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Assignee:
UNIV NAT CORP TOKYO MEDICAL & DENTAL (JP)
International Classes:
A01K67/027; C12N15/09; C12N5/075; C12N5/10; C12N9/16
Domestic Patent References:
WO2016080097A12016-05-26
Foreign References:
JP2016507244A2016-03-10
Other References:
KIM, S. ET AL.: "Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins", GENOME RES., vol. 24, no. 6, 2014, pages 1012 - 1019, XP055277723, ISSN: 1088-9051, DOI: doi:10.1101/gr.171322.113
ARMSTRONG, G.A. ET AL.: "Homology Directed Knockin of Point Mutations in the Zebrafish tardbp and fus Genes in ALS Using the CRISPR/Cas9 System", PLOS ONE, vol. 11, no. 3, March 2016 (2016-03-01), pages e0150188, XP055386255, ISSN: 1932-6203, DOI: doi:10.1371/journal.pone.0150188
NAKAGAWA, Y. ET AL.: "Ultra-superovulation for the CRISPR-Cas9-mediated production of gene -knockout, single-amino-acid-substituted, and floxed mice", BIOL. OPEN, vol. 5, no. 8, July 2016 (2016-07-01), pages 1142 - 1148, XP055516911, ISSN: 2046-6390, DOI: doi:10.1242/bio.019349
HORII, T. ET AL.: "Efficient generation of knockin mice by CRISPR/Cas system", EXP. ANIM., vol. 64, 2015, pages S31, ISSN: 1341-1357
MIURA, H. ET AL.: "CRISPR/Cas9-based generation of knockdown mice by intronic insertion of artificial microRNA using longer single-stranded DNA", SCI. REP., vol. 5, 2015, pages 12799, XP055333089, ISSN: 2045-2322, DOI: doi:10.1038/srep12799
YOSHIMI, K. ET AL.: "ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes", NAT. COMMUN., vol. 7, 20 January 2016 (2016-01-20), pages 10431, XP055361121, ISSN: 2041-1723
AIDA, T. ET AL.: "Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice", GENOME BIOL., vol. 16, 2015, pages 87, XP021215503, ISSN: 1474-7596, DOI: doi:10.1186/s13059-015-0653-x
SVOBODOVA, M. ET AL.: "Comparison of different methods for generation of single-stranded DNA for SELEX processes", ANAL. BIOANAL. CHEM., vol. 404, no. 3, 2012, pages 835 - 842, XP035091549, ISSN: 1618-2642, DOI: doi:10.1007/s00216-012-6183-4
AVCI-ADALI, M. ET AL.: "Upgrading SELEX technology by using lambda exonuclease digestion for single-stranded DNA generation", MOLECULES, vol. 15, no. 1, 24 December 2009 (2009-12-24), pages 1 - 11, XP055603012, ISSN: 1420-3049
Attorney, Agent or Firm:
CENTCREST IP ATTORNEYS (JP)
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