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Title:
METHOD FOR PRODUCING GLUCOSE
Document Type and Number:
WIPO Patent Application WO/2013/137151
Kind Code:
A1
Abstract:
A thermophilic anaerobic microorganism is cultured in the presence of cellulose and β-glucosidase, whereby a cellulase is produced to decompose the cellulose into a saccharide such as cellooligosaccharide, the resultant cellooligosaccharide is further decomposed with β-glucosidase into glucose and the glucose is accumulated in a culture medium. Alternatively, a thermophilic anaerobic microorganism is cultured in the presence of a mixture of starch and cellulose, β-glucosidase, α-amylase and glucoamylase, whereby a cellulase is produced to decompose the starch-cellulose mixture into glucose and the glucose is accumulated in a culture medium.

Inventors:
KOSUGI AKIHIKO (JP)
MORI YUTAKA (JP)
Application Number:
PCT/JP2013/056511
Publication Date:
September 19, 2013
Filing Date:
March 08, 2013
Export Citation:
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Assignee:
JAPAN INTERNAT RES CT FOR AGRICULTURAL SCIENCES (JP)
KOSUGI AKIHIKO (JP)
MORI YUTAKA (JP)
International Classes:
C12P19/02; C12N1/20; C12N15/09; C12P19/14; C12N9/42
Domestic Patent References:
WO2010123932A12010-10-28
Foreign References:
JPS61128898A1986-06-16
JP2011115110A2011-06-16
US20100086981A12010-04-08
JP2010104361A2010-05-13
JP2011004730A2011-01-13
Other References:
WAEONUKUL R ET AL.: "Efficient saccharification of ammonia soaked rice straw by combination of Clostridium thermocellum cellulosome and Thermoanaerobacter brockii p-glucosidase", BIORESOUR TECHNOL., vol. 107, 2 January 2012 (2012-01-02), pages 352 - 357
SPECKA U ET AL.: "Purification and Properties of a Thermoactive Glucoamylase from Clostridium thermosaccharolyticum", APPL ENVIRON MICROBIOL., vol. 57, 1991, pages 2317 - 2323
SEO DJ ET AL.: "Structural changes of lignocelluloses by a nonionic surfactant, Tween 20, and their effects on cellulose adsorption and saccharification", BIORESOUR TECHNOL., vol. 102, 2011, pages 9605 - 9612, XP028290107, DOI: doi:10.1016/j.biortech.2011.07.034
ECKARD AD ET AL.: "Analysis of casein biopolymers adsorption to lignocellulosic biomass as a potential cellulase stabilizer", J BIOMED BIOTECHNOL., vol. 2012, 2012
LI J ET AL.: "The mechanism of poly(ethylene glycol) 4000 effect on enzymatic hydrolysis of lignocellulose", COLLOIDS SURF B BIOINTERFACES., vol. 89, January 2012 (2012-01-01), pages 203 - 210, XP028324031, DOI: doi:10.1016/j.colsurfb.2011.09.019
MAHESHWARI R ET AL.: "Thermophilic Fungi: Their Physiology and Enzymes", MICROBIOL MOL BIOL REV., vol. 64, 2000, pages 461 - 488, XP002674236, DOI: doi:10.1128/MMBR.64.3.461-488.2000
Attorney, Agent or Firm:
HIRAYAMAKazuyuki (JP)
Kazuyuki Hirayama (JP)
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