Title:
METHOD FOR PRODUCING HEMATOPOIETIC CELL FROM PLURIPOTENT STEM CELL
Document Type and Number:
WIPO Patent Application WO/2021/107117
Kind Code:
A1
Abstract:
A method for producing a hematopoietic cell from a pluripotent stem cell, the method including a first step for culturing naïve pluripotent stem cells and inducing differentiation into yolk sac cells, a second step for culturing primed pluripotent stem cells and inducing differentiation into mesoderm cells, and a third step for co-culturing the mesoderm cells obtained in the second step and the yolk sac cells obtained in the first step and thereby inducing differentiation into hematopoietic cells.
Inventors:
TAKASHIMA YASUHIRO (JP)
OKUBO TAKUMI (JP)
OKUBO TAKUMI (JP)
Application Number:
PCT/JP2020/044280
Publication Date:
June 03, 2021
Filing Date:
November 27, 2020
Export Citation:
Assignee:
UNIV KYOTO (JP)
International Classes:
C12N5/078; A61K35/28; A61K35/545; A61P7/06; C12N5/10
Domestic Patent References:
| WO2015199127A1 | 2015-12-30 | |||
| WO2019093340A1 | 2019-05-16 |
Foreign References:
| JP2013521762A | 2013-06-13 | |||
| JP2019512221A | 2019-05-16 |
Other References:
KAUFMAN, D. S. ET AL.: "Hematopoietic colony-forming cells derived from human embryonic stem cells", PNAS, vol. 98, no. 19, 11 September 2001 (2001-09-11), pages 10716 - 10721, XP002308712, DOI: 10.1073/pnas.191362598
YODER, M. C. ET AL.: "Murine Yolk Sac Endoderm- and Mesoderm-Derived Cell Lines Support In Vitro Growth and Differentiation of Hematopoietic Cells", BLOOD, vol. 83, no. 9, 1994, pages 2436 - 2443, XP055832382
KIM, P. G. ET AL.: "Signaling axis involving Hedgehog, Notch, and Scl promotes the embryonic endothelial- to-hematopoietic transition", PNAS, vol. 110, no. 2, 12 December 2012 (2012-12-12), pages E141 - E150, XP055832383
YODER, M. C. ET AL.: "Murine Yolk Sac Endoderm- and Mesoderm-Derived Cell Lines Support In Vitro Growth and Differentiation of Hematopoietic Cells", BLOOD, vol. 83, no. 9, 1994, pages 2436 - 2443, XP055832382
KIM, P. G. ET AL.: "Signaling axis involving Hedgehog, Notch, and Scl promotes the embryonic endothelial- to-hematopoietic transition", PNAS, vol. 110, no. 2, 12 December 2012 (2012-12-12), pages E141 - E150, XP055832383
Attorney, Agent or Firm:
IP FIRM SHUWA (JP)
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