Title:
METHOD FOR PRODUCING HUMAN CORNEAL EPITHELIUM SHEET
Document Type and Number:
WIPO Patent Application WO/2014/142038
Kind Code:
A1
Abstract:
[Problem] To provide a method that is for producing a human corneal epithelium sheet for transplantation to corneal disease patients and that is for culturing, on an amnion substrate, human corneal epithelium-derived cells obtained by culturing human corneal epithelium cells. [Solution] A method for culturing human corneal epithelium cells using human mesenchymal stem cells as support cells, and a method for culturing human corneal epithelium cells using a culture medium to which has been added the combination of a ROCK inhibitor, a phosphodiesterase inhibitor, a MAP kinase inhibitor, and a TGF-β receptor antagonist.
Inventors:
IMAGAWA KIWAMU (JP)
MAEDA KENICHI (JP)
HOSODA YUKI (JP)
YOKOYAMA SHUICHI (JP)
OKUMURA NAOKI (JP)
KOIZUMI NORIKO (JP)
KINOSHITA SHIGERU (JP)
MAEDA KENICHI (JP)
HOSODA YUKI (JP)
YOKOYAMA SHUICHI (JP)
OKUMURA NAOKI (JP)
KOIZUMI NORIKO (JP)
KINOSHITA SHIGERU (JP)
Application Number:
PCT/JP2014/056078
Publication Date:
September 18, 2014
Filing Date:
March 09, 2014
Export Citation:
Assignee:
JAPAN CHEM RES (JP)
KYOTO PREFECTURAL PUBLIC UNIV CORP (JP)
KYOTO PREFECTURAL PUBLIC UNIV CORP (JP)
International Classes:
A61L27/00; C12N5/071
Domestic Patent References:
| WO2005087286A1 | 2005-09-22 |
Foreign References:
| US20060234911A1 | 2006-10-19 | |||
| JPH07500001A | 1995-01-05 | |||
| JP2000506374A | 2000-05-30 | |||
| JP2000517188A | 2000-12-26 |
Other References:
KOIZUMI N. ET AL.: "An evaluation of cultivated corneal limbal epithelial cells, using cell - suspension culture", INVEST. OPHTHALMOL. VIS. SCI., vol. 43, no. 7, July 2002 (2002-07-01), pages 2114 - 2121, XP055279229
OMOTO M. ET AL.: "The use of human mesenchymal stem cell -derived feeder cells for the cultivation of transplantable epithelial sheets", INVEST. OPHTHALMOL. VIS. SCI., vol. 50, no. 5, May 2009 (2009-05-01), pages 2109 - 2115, XP055279242
HIDEYUKI MIYASHITA ET AL.: "ROCK Sogaizai KGF Heiyo ga Kessei Tenka Feeder Kyobaiyo-kei Rinbu Johi Sheet ni Ataeru Koka", JAPAN CORNEA SOCIETY SOKAI KERATOPLASTY SOCIETY OF JAPAN PROGRAM SHOROKUSHU, vol. 36 -28, 2012, pages 59, XP008181303
YAMAGUCHI T. ET AL.: "cAMP stimulates the in vitro proliferation of renal cyst epithelial cells by activating the extracellular signal- regulated kinase pathway", KIDNEY INT., vol. 57, no. 4, April 2000 (2000-04-01), pages 1460 - 1471, XP055192492
KOIZUMI N. ET AL., CORNEA, vol. 19, 2000, pages 65 - 71
KOIZUMI N. ET AL., ARCHIVES OF OPHTHALMOLOGY, vol. 119, 2001, pages 298 - 300
KOIZUMI N. ET AL., THE AMERICAN ACADEMY OF OPHTHALMOLOGY, vol. 108, 2001, pages 1569 - 74
KOIZUMI N. ET AL., INVEST OPHTHALMOL VIS SCI, vol. 43, 2002, pages 2114 - 21
See also references of EP 2975117A4
OMOTO M. ET AL.: "The use of human mesenchymal stem cell -derived feeder cells for the cultivation of transplantable epithelial sheets", INVEST. OPHTHALMOL. VIS. SCI., vol. 50, no. 5, May 2009 (2009-05-01), pages 2109 - 2115, XP055279242
HIDEYUKI MIYASHITA ET AL.: "ROCK Sogaizai KGF Heiyo ga Kessei Tenka Feeder Kyobaiyo-kei Rinbu Johi Sheet ni Ataeru Koka", JAPAN CORNEA SOCIETY SOKAI KERATOPLASTY SOCIETY OF JAPAN PROGRAM SHOROKUSHU, vol. 36 -28, 2012, pages 59, XP008181303
YAMAGUCHI T. ET AL.: "cAMP stimulates the in vitro proliferation of renal cyst epithelial cells by activating the extracellular signal- regulated kinase pathway", KIDNEY INT., vol. 57, no. 4, April 2000 (2000-04-01), pages 1460 - 1471, XP055192492
KOIZUMI N. ET AL., CORNEA, vol. 19, 2000, pages 65 - 71
KOIZUMI N. ET AL., ARCHIVES OF OPHTHALMOLOGY, vol. 119, 2001, pages 298 - 300
KOIZUMI N. ET AL., THE AMERICAN ACADEMY OF OPHTHALMOLOGY, vol. 108, 2001, pages 1569 - 74
KOIZUMI N. ET AL., INVEST OPHTHALMOL VIS SCI, vol. 43, 2002, pages 2114 - 21
See also references of EP 2975117A4
Attorney, Agent or Firm:
SHA Takuho et al. (JP)
謝 卓峰 (JP)
謝 卓峰 (JP)
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