Title:
METHOD FOR PRODUCING INSOLUBLE AGGREGATE OF NEURODEGENERATIVE-DISEASE-RELATED PROTEIN
Document Type and Number:
WIPO Patent Application WO/2013/073219
Kind Code:
A1
Abstract:
The purpose of the present invention is to develop a method for producing a large amount of an insoluble aggregate that is equivalent to an insoluble aggregate formed in the brain of a patient.
A method for producing an insoluble aggregate of a neurodegenerative-disease-related protein according to the present invention comprises the steps of: (1) introducing an insoluble fraction originated from the brain of a neurodegenerative disease patient into a cultured cell in which the neurodegenerative-disease-related protein can be expressed in a constitutive manner; (2) culturing the cultured cell into which the insoluble fraction has been introduced; and (3) extracting an insoluble fraction from the cultured cell. Optionally, the method may additionally comprise a step of amplifying the insoluble aggregate of the neurodegenerative-disease-related protein in the cultured cell.
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Inventors:
NONAKA TAKASHI (JP)
MASUDA MASAMI (JP)
YAMASHITA MAKIKO (JP)
AKIYAMA HARUHIKO (JP)
HASEGAWA MASATO (JP)
MASUDA MASAMI (JP)
YAMASHITA MAKIKO (JP)
AKIYAMA HARUHIKO (JP)
HASEGAWA MASATO (JP)
Application Number:
PCT/JP2012/062794
Publication Date:
May 23, 2013
Filing Date:
May 18, 2012
Export Citation:
Assignee:
TOKYO METROPOLITAN INST MEDICAL SCIENCE (JP)
NONAKA TAKASHI (JP)
MASUDA MASAMI (JP)
YAMASHITA MAKIKO (JP)
AKIYAMA HARUHIKO (JP)
HASEGAWA MASATO (JP)
NONAKA TAKASHI (JP)
MASUDA MASAMI (JP)
YAMASHITA MAKIKO (JP)
AKIYAMA HARUHIKO (JP)
HASEGAWA MASATO (JP)
International Classes:
C07K14/47; C12P21/02; C12Q1/02; C12Q1/06; G01N33/15; G01N33/50; G01N33/68; C12N15/09
Domestic Patent References:
| WO2007066809A1 | 2007-06-14 | |||
| WO2007066809A1 | 2007-06-14 | |||
| WO2009008529A1 | 2009-01-15 |
Other References:
DANZER K.M. ET AL.: "Seeding induced by a- synuclein oligomer sprovides evidence for spreading of a-synuclein pathology.", J. NEUROCHEM., vol. 111, no. 1, 2009, pages 192 - 203, XP055147440
NONAKA T. ET AL.: "Seeded aggregation and toxicity of a-synucle in and Tau: cellular models of neurodegenerative diseases.", J. BIOL. CHEM., vol. 285, no. 45, 2010, pages 34885 - 34898, XP055147441
NONAKA, T. ET AL., J.BIOL.CHEM., vol. 285, 2010, pages 34885
UEDA. K ET AL., PROC. NATL. ACAD. SCI. U. S. A., vol. 90, 1993, pages 11282
OU. SH ET AL., J VIROL., vol. 3584, 1995, pages 394
NONAKA T ET AL., BIOCHEMISTRY, vol. 44, 2005, pages 361
FUJIWARA H ET AL., NAT. CELL BIOL., vol. 4, 2002, pages 160
MASUDA M ET AL., FEBS LETT., vol. 583, 2009, pages 787
GOEDERT M ET AL., NEURON, vol. 3, 1989, pages 519
NONAKA T. ET AL.: "Seeded aggregation and toxicity of a-synucle in and Tau: cellular models of neurodegenerative diseases.", J. BIOL. CHEM., vol. 285, no. 45, 2010, pages 34885 - 34898, XP055147441
NONAKA, T. ET AL., J.BIOL.CHEM., vol. 285, 2010, pages 34885
UEDA. K ET AL., PROC. NATL. ACAD. SCI. U. S. A., vol. 90, 1993, pages 11282
OU. SH ET AL., J VIROL., vol. 3584, 1995, pages 394
NONAKA T ET AL., BIOCHEMISTRY, vol. 44, 2005, pages 361
FUJIWARA H ET AL., NAT. CELL BIOL., vol. 4, 2002, pages 160
MASUDA M ET AL., FEBS LETT., vol. 583, 2009, pages 787
GOEDERT M ET AL., NEURON, vol. 3, 1989, pages 519
Attorney, Agent or Firm:
USHIKI, Mamoru (JP)
牛木 護 (JP)
牛木 護 (JP)
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