Title:
METHOD FOR PRODUCING INSULIN-PRODUCING CELLS, AND COMPOSITION
Document Type and Number:
WIPO Patent Application WO/2020/100788
Kind Code:
A1
Abstract:
The main problem addressed by this invention is to provide a novel production method with which it is possible to efficiently directly transform or derive insulin-producing cells from somatic cells. The present invention is a method for producing insulin-producing cells by directly inducing differentiation from somatic cells, for example, and is characterized by including a step in which somatic cells are cultured in a serum-free medium for induction of differentiation, or is characterized by including a step in which somatic cells are cultured by having the medium for induction of differentiation contain at least 5 μg/mL of insulin. According to this invention, it is possible to directly and efficiently produce insulin-producing cells with a high insulin secretion capacity. The insulin-producing cells obtained by this invention are useful in regenerative medicine and the like.
Inventors:
DAI PING (JP)
TAKEDA YUKIMASA (JP)
HARADA YOSHINORI (JP)
MATSUMOTO JUNICHI (JP)
KUSAKA AYUMI (JP)
TAKEDA YUKIMASA (JP)
HARADA YOSHINORI (JP)
MATSUMOTO JUNICHI (JP)
KUSAKA AYUMI (JP)
Application Number:
PCT/JP2019/044057
Publication Date:
May 22, 2020
Filing Date:
November 11, 2019
Export Citation:
Assignee:
KATAOKA CORP (JP)
KYOTO PREFECTURAL PUBLIC UNIV CORP (JP)
KYOTO PREFECTURAL PUBLIC UNIV CORP (JP)
International Classes:
C12N5/071; C12N5/077; C12N5/0775
Domestic Patent References:
| WO2010055616A1 | 2010-05-20 | |||
| WO2018062269A1 | 2018-04-05 |
Foreign References:
| JP2002348243A | 2002-12-04 |
Other References:
BHAWNA CHANDRAVANSHI, RAMESH BHONDE: "Reprogramming mouse embryo fibroblasts to functional islets without genetic manipulation", JOURNAL OF CELLULAR PHYSIOLOGY, vol. 233, no. 2, 28 June 2017 (2017-06-28), pages 1627 - 1637, XP055819513, ISSN: 0021-9541, DOI: 10.1002/jcp.26068
ANANDWARDHAN A. HARDIKAR, BERNICE MARCUS-SAMUELS, ELIZABETH GERAS-RAAKA, BRUCE M. RAAKA, MARVIN C. GERSHENGORN: "Human pancreatic precursor cells secrete FGF2 to stimulate clustering into hormone-expressing islet-like cell aggregates", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCE, vol. 100, no. 12, 10 June 2003 (2003-06-10), pages 7117 - 7122, XP055819515, ISSN: 0027-8424, DOI: 10.1073/pnas.1232230100
AMIT CHOUDHARY, KAIHUI HU HE, PHILIPP MERTINS, NAMRATA D. UDESHI, VLADO DANČÍK, DINA FOMINA-YADLIN, STEFAN KUBICEK, PAUL A. CLEMON: "Quantitative-Proteomic Comparison of Alpha and Beta Cells to Uncover Novel Targets for Lineage Reprogramming", PLOS ONE, vol. 9, no. 4, e95194, 23 April 2014 (2014-04-23), pages 1 - 10, XP055707997, DOI: 10.1371/journal.pone.0095194
TAIOKA TOSHIHIRO: "Molecular mechanisms of insulin resistance in beta cells.", THE SHOWA UNIVERSITY JOURNAL OF MEDICAL SCIENCES, vol. 2, no. 2, 2011, pages 127 - 138, XP009528083, ISSN: 1884-7854
XIAOJIE MA, SAIYONG ZHU: "Chemical strategies for pancreatic beta cell differentiation, reprogramming, and regeneration", ACTA BIOCHIMICA BIOPHYSICA SINICA, vol. 49, no. 4, 1 April 2017 (2017-04-01), pages 289 - 301, XP055647725, ISSN: 1672-9145, DOI: 10.1093/abbs/gmx008
CURRENT PATHOBIOLOGY REPORTS, vol. 3, 2016, pages 57 - 65
CELL STEM CELL, vol. 14, 2014, pages 228 - 236
NATURE COMMUNICATIONS, vol. 7, 2016, pages 10080
JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 292, 2017, pages 19122 - 19132
CAS, no. 1620576-64-8
CAS , no. 603288-22-8
ANANDWARDHAN A. HARDIKAR, BERNICE MARCUS-SAMUELS, ELIZABETH GERAS-RAAKA, BRUCE M. RAAKA, MARVIN C. GERSHENGORN: "Human pancreatic precursor cells secrete FGF2 to stimulate clustering into hormone-expressing islet-like cell aggregates", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCE, vol. 100, no. 12, 10 June 2003 (2003-06-10), pages 7117 - 7122, XP055819515, ISSN: 0027-8424, DOI: 10.1073/pnas.1232230100
AMIT CHOUDHARY, KAIHUI HU HE, PHILIPP MERTINS, NAMRATA D. UDESHI, VLADO DANČÍK, DINA FOMINA-YADLIN, STEFAN KUBICEK, PAUL A. CLEMON: "Quantitative-Proteomic Comparison of Alpha and Beta Cells to Uncover Novel Targets for Lineage Reprogramming", PLOS ONE, vol. 9, no. 4, e95194, 23 April 2014 (2014-04-23), pages 1 - 10, XP055707997, DOI: 10.1371/journal.pone.0095194
TAIOKA TOSHIHIRO: "Molecular mechanisms of insulin resistance in beta cells.", THE SHOWA UNIVERSITY JOURNAL OF MEDICAL SCIENCES, vol. 2, no. 2, 2011, pages 127 - 138, XP009528083, ISSN: 1884-7854
XIAOJIE MA, SAIYONG ZHU: "Chemical strategies for pancreatic beta cell differentiation, reprogramming, and regeneration", ACTA BIOCHIMICA BIOPHYSICA SINICA, vol. 49, no. 4, 1 April 2017 (2017-04-01), pages 289 - 301, XP055647725, ISSN: 1672-9145, DOI: 10.1093/abbs/gmx008
CURRENT PATHOBIOLOGY REPORTS, vol. 3, 2016, pages 57 - 65
CELL STEM CELL, vol. 14, 2014, pages 228 - 236
NATURE COMMUNICATIONS, vol. 7, 2016, pages 10080
JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 292, 2017, pages 19122 - 19132
CAS, no. 1620576-64-8
CAS , no. 603288-22-8
Attorney, Agent or Firm:
SHIMIZU Naoto (JP)
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