Title:
METHOD FOR PRODUCING INTESTINAL CELLS FROM PLURIPOTENT STEM CELLS
Document Type and Number:
WIPO Patent Application WO/2020/054659
Kind Code:
A1
Abstract:
Provided is a method for producing intestinal cells derived from pluripotent stem cells, the produced intestinal cells exhibiting functions which are similar to those of actual intestinal cells. This method for producing intestinal cells uses a culture for differentiation into intestinal cells, which is characterized by containing a GSK3 inhibitor and one type selected from the group consisting of an activating agent for hepatocyte growth factor receptors, adrenal cortex hormone, calcitriol, and dimethylsulfoxide.
More Like This:
Inventors:
KUME SHOEN (JP)
SHIRAKI NOBUAKI (JP)
MAEDA KAZUYA (JP)
KUSUHARA HIROYUKI (JP)
ISHIKAWA MASAYA (JP)
WATANABE TERUHIKO (JP)
SHIRAKI NOBUAKI (JP)
MAEDA KAZUYA (JP)
KUSUHARA HIROYUKI (JP)
ISHIKAWA MASAYA (JP)
WATANABE TERUHIKO (JP)
Application Number:
PCT/JP2019/035358
Publication Date:
March 19, 2020
Filing Date:
September 09, 2019
Export Citation:
Assignee:
TOKYO INST TECH (JP)
UNIV TOKYO (JP)
KANTO KAGAKU (JP)
UNIV TOKYO (JP)
KANTO KAGAKU (JP)
International Classes:
C12N5/071; C07K14/78; C12N1/00; C12N5/074
Domestic Patent References:
| WO2012060315A1 | 2012-05-10 | |||
| WO2016147975A1 | 2016-09-22 | |||
| WO2019021990A1 | 2019-01-31 | |||
| WO2015125662A1 | 2015-08-27 | |||
| WO2012026531A1 | 2012-03-01 |
Foreign References:
| JP2005525104A | 2005-08-25 | |||
| JP2009525030A | 2009-07-09 | |||
| JP2016530219A | 2016-09-29 | |||
| JP2018168957A | 2018-11-01 | |||
| JP2019117748A | 2019-07-18 | |||
| JP2012115262A | 2012-06-21 |
Other References:
OGAKI, S. ET AL.: "Wnt and Notch Signals Guide Embryonic Stem Cell Differentiation into the Intestinal Lineages", STEM CELLS, vol. 31, 2013, pages 1086 - 1096, XP055177743, DOI: 10.1002/stem.1344
HUBATSCH, I.RAGNARSSON, E. G. E.ARTURSSON, P.: "Determination of drug permeability and prediction of drug absorption in Caco-2 monolayers", NAT. PROTOC., vol. 2, 2007, pages 2111 - 2119
SUN, D.LENNERNAS, H.WELAGE, L. S.BARNETT, J. L.LANDOWSKI, C. P.FOSTER, D.FLEISHER, D.LEE, K. D.AMIDON, G. L.: "Comparison of human duodenum and Caco-2 gene expression profiles for 12,000 gene sequences tags and correlation with permeability of 26 drugs", PHARM. RES., vol. 19, 2002, pages 4 - 6
TAKAHASHI, K.TANABE, K.OHNUKI, M.NARITA, M.ICHISAKA, T.TOMODA, K.YAMANAKA, S.: "Induction of pluripotent stem cells from adult human fibroblasts by defined factors", CELL, vol. 131, 2007, pages 861 - 872, XP055547222, DOI: 10.1016/j.cell.2007.11.019
NAKAMURA, T.TSUCHIYA, K.WATANABE, M.: "Crosstalk between Wnt and Notch signaling in intestinal epithelial cell fate decision", J. GASTROENTEROL., vol. 42, 2007, pages 705 - 10, XP019543723, DOI: 10.1007/s00535-007-2087-z
SATO, T.CLEVERS, H.: "Growing Self-Organized Mini-Guts from a Single Intestinal Stem Cell: Mechanisms and Applications", SCIENCE, vol. 340, 2013, pages 190 - 194
BUCZACKI, S. J.ZECCHINI, H. I.NICHOLSON, A. M.RUSSELL, R.VERMEULEN, L.KEMP, R.WINTON, D. J.: "Intestinal label-retaining cells are secretory precursors expressing Lgr5", NATURE, vol. 495, 2013, pages 65 - 9, XP055407429, DOI: 10.1038/nature11965
CHIBA, S.: "Notch signaling in stem cell systems", STEM CELLS, vol. 24, 2006, pages 2437 - 47
FRE, S.HUYGHE, M.MOURIKIS, P.ROBINE, S.LOUVARD, D.ARTAVANIS-TSAKONAS, S.: "Notch signals control the fate of immature progenitor cells in the intestine", NATURE, vol. 435, 2005, pages 964 - 8, XP055407428, DOI: 10.1038/nature03589
FRE, S.PALLAVI, S. K.HUYGHE, M.LAE, M.JANSSEN, K.-P.ROBINE, S.ARTAVANIS-TSAKONAS, S.LOUVARD, D.: "Notch and Wnt signals cooperatively control cell proliferation and tumorigenesis in the intestine", PROC. NATL. ACAD. SCI. U. S. A., vol. 106, 2009, pages 6309 - 14, XP055122117, DOI: 10.1073/pnas.0900427106
GERBE, F.VAN ES, J. H.MAKRINI, L.BRULIN, B.MELLITZER, G.ROBINE, S.ROMAGNOLO, B.SHROYER, N. F.BOURGAUX, J.-F.PIGNODEL, C. ET AL.: "Distinct ATOH1 and Neurog3 requirements define tuft cells as a new secretory cell type in the intestinal epithelium", J. CELL BIOL., vol. 192, 2011, pages 767 - 80
GREGORIEFF, A.LIU, Y.INANLOU, M. R.KHOMCHUK, Y.WRANA, J. L.: "Yap-dependent reprogramming of Lgr5+ stem cells drives intestinal regeneration and cancer", NATURE, 2015
BARKER, N.VAN ES, J. H.KUIPERS, J.KUJALA, P.VAN DEN BORN, M.COZIJNSEN, M.HAEGEBARTH, A.KORVING, J.BEGTHEL, H.PETERS, P. J. ET AL.: "Identification of stem cells in small intestine and colon by marker gene Lgr5", NATURE, vol. 449, 2007, pages 1003 - 146
SATO, T.VRIES, R. G.SNIPPERT, H. J.VAN DE WETERING, M.BARKER, N.STANGE, D. E.VAN ES, J. H.ABO, A.KUJALA, P.PETERS, P. J. ET AL.: "Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche", NATURE, vol. 459, 2009, pages 262 - 5, XP055183787, DOI: 10.1038/nature07935
SATO, T.STANGE, D. E.FERRANTE, M.VRIES, R. G. J.VAN ES, J. H.VAN DEN BRINK, S.VAN HOUDT, W. J.PRONK, A.VAN GORP, J.SIERSEMA, P. D.: "Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett's epithelium", GASTROENTEROLOGY, vol. 141, 2011, pages 1762 - 1772, XP028325676, DOI: 10.1053/j.gastro.2011.07.050
SPENCE, J. R.MAYHEW, C. N.RANKIN, S.KUHAR, M. F.VALLANCE, J. E.TOLLE, K.HOSKINS, E. E.KALINICHENKO, V. VWELLS, S. I.ZORN, A. M. ET: "Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro", NATURE, vol. 470, 2011, pages 105 - 9
WATSON, C. L.MAHE, M. M.MUNERA, J.HOWELL, J. C.SUNDARAM, N.POLING, H. M.SCHWEITZER, J. I.VALLANCE, J. E.MAYHEW, C. N.SUN, Y. ET AL: "An in vivo model of human small intestine using pluripotent stem cells", NAT. MED., vol. 20, 2014, XP055241417, DOI: 10.1038/nm.3737
AMERI, J.STAHLBERG, A.PEDERSEN, J.JOHANSSON, J. K.JOHANNESSON, M. M.ARTNER, I.SEMB, H.: "FGF2 specifies hESC-derived definitive endoderm into foregut/midgut cell lineages in a concentration-dependent manner", STEM CELLS, vol. 28, 2010, pages 45 - 56, XP002610827, DOI: 10.1002/stem.249
HANSSON, M.OLESEN, D. R.PETERSLUND, J. M. L.ENGBERG, N.KAHN, M.WINZI, M.KLEIN, T.MADDOX-HYTTEL, P.SERUP, P.: "A late requirement for Wnt and FGF signaling during activin-induced formation of foregut endoderm from mouse embryonic stem cells", DEV. BIOL., vol. 330, 2009, pages 286 - 304, XP026130754, DOI: 10.1016/j.ydbio.2009.03.026
OGAKI, S.SHIRAKI, N.KUME, K.KUME, S.: "Wnt and Notch signals guide embryonic stem cell differentiation into the intestinal lineages", STEM CELLS, vol. 31, 2013, pages 1086 - 96, XP055177743, DOI: 10.1002/stem.1344
OGAKI, S.MOROOKA, M.OTERA, K.KUME, S.: "A cost-effective system for differentiation of intestinal epithelium from human induced pluripotent stem cells", SCI. REP., vol. 5, 2015, pages 17297, XP055641733, DOI: 10.1038/srep17297
IWAO, T.KODAMA, N.KONDO, Y.KABEYA, T.NAKAMURA, K.HORIKAWA, T.NIWA, T.KUROSE, K.MATSUNAGA, T.: "Generation of Enterocyte-Like Cells with Pharmacokinetic Functions from Human Induced Pluripotent Stem Cells Using Small- Molecule Compounds", DRUG METAB. DISPOS., vol. 43, 2015, pages 603 - 610, XP055415683, DOI: 10.1124/dmd.114.062604
KODAMA, N.IWAO, T.KATANO, T.OHTA, K.YUASA, H.MATSUNAGA, T.: "Characteristic Analysis of Intestinal Transport in Enterocyte-Like Cells Differentiated from Human Induced Pluripotent Stem Cells", DRUG METAB. DISPOS., vol. 44, 2016, pages 0 - 0
NEGORO, R. ET AL., RES. COMMUN., vol. 472, 2016, pages 631 - 6
TAKAHASHI ET AL., CELL, vol. 126, 2006, pages 663 - 676
MAHERALI ET AL., CELL STEM CELL, vol. 1, 2007, pages 55 - 70
NAKAGAWA ET AL., NAT BIOTECHNOL, vol. 26, 2008, pages 101 - 106
WERNIG ET AL., CELL STEM CELL, vol. 10, 2008, pages 10 - 12
YU ET AL., SCIENCE, vol. 318, 2007, pages 1917 - 1920
STADTFELD ET AL., SCIENCE, vol. 322, 2008, pages 945 - 949
SHIRAKI ET AL., STEM CELLS, vol. 26, 2008, pages 874 - 85
TOSHIAKI TAKEZAWA, THE SOCIETY FOR BIOTECHNOLOGY BROCHURE, vol. 91, 2013, pages 214 - 217
ESTUDANTE, M.MORAIS, J. G.SOVERAL, G.BENET, L. Z.: "Intestinal drug transporters: An overview", ADV. DRUG DELIV. REV., vol. 65, 2013, pages 1340 - 1356, XP028737353, DOI: 10.1016/j.addr.2012.09.042
DJUV, A.NILSEN, 0. G.: "Review of pharmacological effects of Glycyrrhiza radix and its bioactive compounds", PHYTOTHER. RES., vol. 22, 2008, pages 1623 - 1628
WRIGHT, J. A.HASLAM, I. S.COLEMAN, T.SIMMONS, N. L.: "Breast cancer resistance protein BCRP (ABCG2)-mediated transepithelial nitrofurantoin secretion and its regulation in human intestinal epithelial (Caco-2) layers", EUR. J. PHARMACOL., vol. 672, 2011, pages 70 - 76, XP028113179, DOI: 10.1016/j.ejphar.2011.10.004
COGBURN JN ET AL., PHARM RES, vol. 8, 1991, pages 210 - 216
ARTURSSON, J. PHARM. SCI., vol. 79, 1990, pages 476 - 482
OSHIKATA-MIYAZAKI, A.TAKEZAWA, T.: "Development of an oxygenation culture method for activating the liver-specific functions of HepG2 cells utilizing a collagen vitrigel membrane chamber", CYTOTECHNOLOGY, vol. 68, 2016, pages 1801 - 1811, XP036055355, DOI: 10.1007/s10616-015-9934-1
YAMAGUCHI, H.KOJIMA, H.TAKEZAWA, T.: "Vitrigel-eye irritancy test method using HCE-T cells", TOXICOL. SCI., vol. 135, 2013, pages 347 - 355
YAMAGUCHI, H.KOJIMA, H.TAKEZAWA, T.: "Predictive performance of the Vitrigel-eye irritancy test method using 118 chemicals", J. APPL. TOXICOL., vol. 36, 2016, pages 1025 - 1037
CAS, no. 59865-13-3
ARTURSSON, P.: "Epithelial Transport Of Drugs In Cell Culture. I: A Model For Studying The Passive Diffusion Of Drugs Over Intestinal Absorbtive (Caco-2", CELLS. J. PHARM. SCI., vol. 79, 1990, pages 476 - 482
ASPLUND, A.PRADIP, A.VAN GIEZEN, M.ASPEGREN, A.CHOUKAIR, H.REHNSTROM, M.JACOBSSON, S.GHOSHEH, N.EL HAJJAM, D.HOLMGREN, S. ET AL.: "One Standardized Differentiation Procedure Robustly Generates Homogenous Hepatocyte Cultures Displaying Metabolic Diversity from a Large Panel of Human Pluripotent Stem Cells", STEM CELL REV. REPORTS., 2015
COGBURN, J. N.DONOVAN, M. G.SCHASTEEN, C. S.: "A model of human small intestinal absorptive cells", TRANSPORT BARRIER. PHARM. RES., vol. 8, 1991, pages 210 - 6
LANCASTER, M. A.KNOBLICH, J. A.: "Organogenesis in a dish: modeling development and disease using organoid technologies", SCIENCE, vol. 345, 2014, pages 1247125
NEGORO, R.TAKAYAMA, K.NAGAMOTO, Y.SAKURAI, F.TACHIBANA, M.MIZUGUCHI, H.: "Modeling of drug-mediated CYP3A4 induction by using human iPS cell-derived enterocyte-like cells", BIOCHEM. BIOPHYS. RES. COMMUN., vol. 472, 2016, pages 631 - 6, XP029475186, DOI: 10.1016/j.bbrc.2016.03.012
TAKANO, J.MAEDA, K.BOLGER, M. B.SUGIYAMA, Y.: "The prediction of the relative importance of CYP3A/P-glycoprotein to the nonlinear intestinal absorption of drugs by advanced compartmental absorption and transit model", DRUG METAB. DISPOS., vol. 44, 2016, pages 1808 - 1818
See also references of EP 3851518A4
HUBATSCH, I.RAGNARSSON, E. G. E.ARTURSSON, P.: "Determination of drug permeability and prediction of drug absorption in Caco-2 monolayers", NAT. PROTOC., vol. 2, 2007, pages 2111 - 2119
SUN, D.LENNERNAS, H.WELAGE, L. S.BARNETT, J. L.LANDOWSKI, C. P.FOSTER, D.FLEISHER, D.LEE, K. D.AMIDON, G. L.: "Comparison of human duodenum and Caco-2 gene expression profiles for 12,000 gene sequences tags and correlation with permeability of 26 drugs", PHARM. RES., vol. 19, 2002, pages 4 - 6
TAKAHASHI, K.TANABE, K.OHNUKI, M.NARITA, M.ICHISAKA, T.TOMODA, K.YAMANAKA, S.: "Induction of pluripotent stem cells from adult human fibroblasts by defined factors", CELL, vol. 131, 2007, pages 861 - 872, XP055547222, DOI: 10.1016/j.cell.2007.11.019
NAKAMURA, T.TSUCHIYA, K.WATANABE, M.: "Crosstalk between Wnt and Notch signaling in intestinal epithelial cell fate decision", J. GASTROENTEROL., vol. 42, 2007, pages 705 - 10, XP019543723, DOI: 10.1007/s00535-007-2087-z
SATO, T.CLEVERS, H.: "Growing Self-Organized Mini-Guts from a Single Intestinal Stem Cell: Mechanisms and Applications", SCIENCE, vol. 340, 2013, pages 190 - 194
BUCZACKI, S. J.ZECCHINI, H. I.NICHOLSON, A. M.RUSSELL, R.VERMEULEN, L.KEMP, R.WINTON, D. J.: "Intestinal label-retaining cells are secretory precursors expressing Lgr5", NATURE, vol. 495, 2013, pages 65 - 9, XP055407429, DOI: 10.1038/nature11965
CHIBA, S.: "Notch signaling in stem cell systems", STEM CELLS, vol. 24, 2006, pages 2437 - 47
FRE, S.HUYGHE, M.MOURIKIS, P.ROBINE, S.LOUVARD, D.ARTAVANIS-TSAKONAS, S.: "Notch signals control the fate of immature progenitor cells in the intestine", NATURE, vol. 435, 2005, pages 964 - 8, XP055407428, DOI: 10.1038/nature03589
FRE, S.PALLAVI, S. K.HUYGHE, M.LAE, M.JANSSEN, K.-P.ROBINE, S.ARTAVANIS-TSAKONAS, S.LOUVARD, D.: "Notch and Wnt signals cooperatively control cell proliferation and tumorigenesis in the intestine", PROC. NATL. ACAD. SCI. U. S. A., vol. 106, 2009, pages 6309 - 14, XP055122117, DOI: 10.1073/pnas.0900427106
GERBE, F.VAN ES, J. H.MAKRINI, L.BRULIN, B.MELLITZER, G.ROBINE, S.ROMAGNOLO, B.SHROYER, N. F.BOURGAUX, J.-F.PIGNODEL, C. ET AL.: "Distinct ATOH1 and Neurog3 requirements define tuft cells as a new secretory cell type in the intestinal epithelium", J. CELL BIOL., vol. 192, 2011, pages 767 - 80
GREGORIEFF, A.LIU, Y.INANLOU, M. R.KHOMCHUK, Y.WRANA, J. L.: "Yap-dependent reprogramming of Lgr5+ stem cells drives intestinal regeneration and cancer", NATURE, 2015
BARKER, N.VAN ES, J. H.KUIPERS, J.KUJALA, P.VAN DEN BORN, M.COZIJNSEN, M.HAEGEBARTH, A.KORVING, J.BEGTHEL, H.PETERS, P. J. ET AL.: "Identification of stem cells in small intestine and colon by marker gene Lgr5", NATURE, vol. 449, 2007, pages 1003 - 146
SATO, T.VRIES, R. G.SNIPPERT, H. J.VAN DE WETERING, M.BARKER, N.STANGE, D. E.VAN ES, J. H.ABO, A.KUJALA, P.PETERS, P. J. ET AL.: "Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche", NATURE, vol. 459, 2009, pages 262 - 5, XP055183787, DOI: 10.1038/nature07935
SATO, T.STANGE, D. E.FERRANTE, M.VRIES, R. G. J.VAN ES, J. H.VAN DEN BRINK, S.VAN HOUDT, W. J.PRONK, A.VAN GORP, J.SIERSEMA, P. D.: "Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett's epithelium", GASTROENTEROLOGY, vol. 141, 2011, pages 1762 - 1772, XP028325676, DOI: 10.1053/j.gastro.2011.07.050
SPENCE, J. R.MAYHEW, C. N.RANKIN, S.KUHAR, M. F.VALLANCE, J. E.TOLLE, K.HOSKINS, E. E.KALINICHENKO, V. VWELLS, S. I.ZORN, A. M. ET: "Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro", NATURE, vol. 470, 2011, pages 105 - 9
WATSON, C. L.MAHE, M. M.MUNERA, J.HOWELL, J. C.SUNDARAM, N.POLING, H. M.SCHWEITZER, J. I.VALLANCE, J. E.MAYHEW, C. N.SUN, Y. ET AL: "An in vivo model of human small intestine using pluripotent stem cells", NAT. MED., vol. 20, 2014, XP055241417, DOI: 10.1038/nm.3737
AMERI, J.STAHLBERG, A.PEDERSEN, J.JOHANSSON, J. K.JOHANNESSON, M. M.ARTNER, I.SEMB, H.: "FGF2 specifies hESC-derived definitive endoderm into foregut/midgut cell lineages in a concentration-dependent manner", STEM CELLS, vol. 28, 2010, pages 45 - 56, XP002610827, DOI: 10.1002/stem.249
HANSSON, M.OLESEN, D. R.PETERSLUND, J. M. L.ENGBERG, N.KAHN, M.WINZI, M.KLEIN, T.MADDOX-HYTTEL, P.SERUP, P.: "A late requirement for Wnt and FGF signaling during activin-induced formation of foregut endoderm from mouse embryonic stem cells", DEV. BIOL., vol. 330, 2009, pages 286 - 304, XP026130754, DOI: 10.1016/j.ydbio.2009.03.026
OGAKI, S.SHIRAKI, N.KUME, K.KUME, S.: "Wnt and Notch signals guide embryonic stem cell differentiation into the intestinal lineages", STEM CELLS, vol. 31, 2013, pages 1086 - 96, XP055177743, DOI: 10.1002/stem.1344
OGAKI, S.MOROOKA, M.OTERA, K.KUME, S.: "A cost-effective system for differentiation of intestinal epithelium from human induced pluripotent stem cells", SCI. REP., vol. 5, 2015, pages 17297, XP055641733, DOI: 10.1038/srep17297
IWAO, T.KODAMA, N.KONDO, Y.KABEYA, T.NAKAMURA, K.HORIKAWA, T.NIWA, T.KUROSE, K.MATSUNAGA, T.: "Generation of Enterocyte-Like Cells with Pharmacokinetic Functions from Human Induced Pluripotent Stem Cells Using Small- Molecule Compounds", DRUG METAB. DISPOS., vol. 43, 2015, pages 603 - 610, XP055415683, DOI: 10.1124/dmd.114.062604
KODAMA, N.IWAO, T.KATANO, T.OHTA, K.YUASA, H.MATSUNAGA, T.: "Characteristic Analysis of Intestinal Transport in Enterocyte-Like Cells Differentiated from Human Induced Pluripotent Stem Cells", DRUG METAB. DISPOS., vol. 44, 2016, pages 0 - 0
NEGORO, R. ET AL., RES. COMMUN., vol. 472, 2016, pages 631 - 6
TAKAHASHI ET AL., CELL, vol. 126, 2006, pages 663 - 676
MAHERALI ET AL., CELL STEM CELL, vol. 1, 2007, pages 55 - 70
NAKAGAWA ET AL., NAT BIOTECHNOL, vol. 26, 2008, pages 101 - 106
WERNIG ET AL., CELL STEM CELL, vol. 10, 2008, pages 10 - 12
YU ET AL., SCIENCE, vol. 318, 2007, pages 1917 - 1920
STADTFELD ET AL., SCIENCE, vol. 322, 2008, pages 945 - 949
SHIRAKI ET AL., STEM CELLS, vol. 26, 2008, pages 874 - 85
TOSHIAKI TAKEZAWA, THE SOCIETY FOR BIOTECHNOLOGY BROCHURE, vol. 91, 2013, pages 214 - 217
ESTUDANTE, M.MORAIS, J. G.SOVERAL, G.BENET, L. Z.: "Intestinal drug transporters: An overview", ADV. DRUG DELIV. REV., vol. 65, 2013, pages 1340 - 1356, XP028737353, DOI: 10.1016/j.addr.2012.09.042
DJUV, A.NILSEN, 0. G.: "Review of pharmacological effects of Glycyrrhiza radix and its bioactive compounds", PHYTOTHER. RES., vol. 22, 2008, pages 1623 - 1628
WRIGHT, J. A.HASLAM, I. S.COLEMAN, T.SIMMONS, N. L.: "Breast cancer resistance protein BCRP (ABCG2)-mediated transepithelial nitrofurantoin secretion and its regulation in human intestinal epithelial (Caco-2) layers", EUR. J. PHARMACOL., vol. 672, 2011, pages 70 - 76, XP028113179, DOI: 10.1016/j.ejphar.2011.10.004
COGBURN JN ET AL., PHARM RES, vol. 8, 1991, pages 210 - 216
ARTURSSON, J. PHARM. SCI., vol. 79, 1990, pages 476 - 482
OSHIKATA-MIYAZAKI, A.TAKEZAWA, T.: "Development of an oxygenation culture method for activating the liver-specific functions of HepG2 cells utilizing a collagen vitrigel membrane chamber", CYTOTECHNOLOGY, vol. 68, 2016, pages 1801 - 1811, XP036055355, DOI: 10.1007/s10616-015-9934-1
YAMAGUCHI, H.KOJIMA, H.TAKEZAWA, T.: "Vitrigel-eye irritancy test method using HCE-T cells", TOXICOL. SCI., vol. 135, 2013, pages 347 - 355
YAMAGUCHI, H.KOJIMA, H.TAKEZAWA, T.: "Predictive performance of the Vitrigel-eye irritancy test method using 118 chemicals", J. APPL. TOXICOL., vol. 36, 2016, pages 1025 - 1037
CAS, no. 59865-13-3
ARTURSSON, P.: "Epithelial Transport Of Drugs In Cell Culture. I: A Model For Studying The Passive Diffusion Of Drugs Over Intestinal Absorbtive (Caco-2", CELLS. J. PHARM. SCI., vol. 79, 1990, pages 476 - 482
ASPLUND, A.PRADIP, A.VAN GIEZEN, M.ASPEGREN, A.CHOUKAIR, H.REHNSTROM, M.JACOBSSON, S.GHOSHEH, N.EL HAJJAM, D.HOLMGREN, S. ET AL.: "One Standardized Differentiation Procedure Robustly Generates Homogenous Hepatocyte Cultures Displaying Metabolic Diversity from a Large Panel of Human Pluripotent Stem Cells", STEM CELL REV. REPORTS., 2015
COGBURN, J. N.DONOVAN, M. G.SCHASTEEN, C. S.: "A model of human small intestinal absorptive cells", TRANSPORT BARRIER. PHARM. RES., vol. 8, 1991, pages 210 - 6
LANCASTER, M. A.KNOBLICH, J. A.: "Organogenesis in a dish: modeling development and disease using organoid technologies", SCIENCE, vol. 345, 2014, pages 1247125
NEGORO, R.TAKAYAMA, K.NAGAMOTO, Y.SAKURAI, F.TACHIBANA, M.MIZUGUCHI, H.: "Modeling of drug-mediated CYP3A4 induction by using human iPS cell-derived enterocyte-like cells", BIOCHEM. BIOPHYS. RES. COMMUN., vol. 472, 2016, pages 631 - 6, XP029475186, DOI: 10.1016/j.bbrc.2016.03.012
TAKANO, J.MAEDA, K.BOLGER, M. B.SUGIYAMA, Y.: "The prediction of the relative importance of CYP3A/P-glycoprotein to the nonlinear intestinal absorption of drugs by advanced compartmental absorption and transit model", DRUG METAB. DISPOS., vol. 44, 2016, pages 1808 - 1818
See also references of EP 3851518A4
Attorney, Agent or Firm:
NOMURA, Kenichi et al. (JP)
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