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Title:
METHOD OF PRODUCING ISOPRENE MONOMER
Document Type and Number:
WIPO Patent Application WO/2015/147341
Kind Code:
A3
Abstract:
A host cell includes a heterogeneous expression unit including: (a) a polynucleotide encoding a mevalonate kinase derived from a microorganism belonging to a genus selected from Methanocella, Corynebacterium, Methanosaeta, and Nitrosopumilus, and (b) a promoter operatively linked to the polynucleotide. The host cell is used to produce mevalonate kinase, mevalonate-5-phosphate, and isoprenoid compounds.

Inventors:
MIHARA YOKO (JP)
RACHI HIROAKI (JP)
NISHIO YOSUKE (JP)
KATASHKINA YOSIFOVNA JOANNA (RU)
KAZIEVA DMITRIEVNA EKATERINA (RU)
ANDREEVA GEORGIEVNA IRINA (RU)
Application Number:
PCT/JP2015/060529
Publication Date:
November 19, 2015
Filing Date:
March 27, 2015
Export Citation:
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Assignee:
AJINOMOTO KK (JP)
International Classes:
C12N1/21; C12N9/12; C12P5/00; C12P9/00
Domestic Patent References:
WO2010031062A12010-03-18
Foreign References:
EP2218729A12010-08-18
US5914245A1999-06-22
Other References:
MATSUMI RIE ET AL: "Isoprenoid biosynthesis in Archaea - Biochemical and evolutionary implications", RESEARCH IN MICROBIOLOGY, vol. 162, no. 1, Sp. Iss. SI, January 2011 (2011-01-01), pages 39 - 52, XP002740471, ISSN: 0923-2508
DATABASE UniProt [online] 9 February 2010 (2010-02-09), "RecName: Full=Mevalonate kinase {ECO:0000256|HAMAP-Rule:MF_00217}; Short=MK {ECO:0000256|HAMAP-Rule:MF_00217}; Short=MVK {ECO:0000256|HAMAP-Rule:MF_00217}; EC=2.7.1.36 {ECO:0000256|HAMAP-Rule:MF_00217};", XP002740472, retrieved from EBI accession no. UNIPROT:D1YZ39 Database accession no. D1YZ39
DATABASE UniProt [online] 19 October 2011 (2011-10-19), "SubName: Full=Mevalonate kinase {ECO:0000313|EMBL:AEK36255.1}; EC=2.7.1.36 {ECO:0000313|EMBL:AEK36255.1};", XP002744231, retrieved from EBI accession no. UNIPROT:G0HC93 Database accession no. G0HC93
DATABASE UniProt [online] 28 June 2011 (2011-06-28), "RecName: Full=Mevalonate kinase {ECO:0000256|HAMAP-Rule:MF_00217}; Short=MK {ECO:0000256|HAMAP-Rule:MF_00217}; Short=MVK {ECO:0000256|HAMAP-Rule:MF_00217}; EC=2.7.1.36 {ECO:0000256|HAMAP-Rule:MF_00217};", XP002744232, retrieved from EBI accession no. UNIPROT:F4BZB3 Database accession no. F4BZB3
DATABASE UniProt [online] 15 January 2008 (2008-01-15), "RecName: Full=Mevalonate kinase {ECO:0000256|HAMAP-Rule:MF_00217}; Short=MK {ECO:0000256|HAMAP-Rule:MF_00217}; Short=MVK {ECO:0000256|HAMAP-Rule:MF_00217}; EC=2.7.1.36 {ECO:0000256|HAMAP-Rule:MF_00217};", XP002744233, retrieved from EBI accession no. UNIPROT:A9A427 Database accession no. A9A427
See also references of EP 3122868A2
Attorney, Agent or Firm:
SAKAI, Hiroaki et al. (Toranomon Mitsui Building 8-1, Kasumigaseki 3-chome, Chiyoda-k, Tokyo 13, JP)
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