Title:
METHOD FOR PRODUCING KERATINOCYTES, MEDIUM KIT FOR CONTINUOUSLY CULTURING KERATINOCYTES, AND KERATINOCYTES
Document Type and Number:
WIPO Patent Application WO/2024/024561
Kind Code:
A1
Abstract:
This method for producing keratinocytes includes a separation step that is repeated multiple times, in which step trypsinization is performed on a cell population including keratinocytes induced from pluripotent stem cells, to separate keratinocytes. The present invention realizes a technique of producing a cell population of keratinocytes induced from pluripotent stem cells, the cell population including keratinocytes having high proliferation capacity.
Inventors:
UMEZAWA AKIHIRO (JP)
TAKAGI RYO (JP)
TAKAGI RYO (JP)
Application Number:
PCT/JP2023/026201
Publication Date:
February 01, 2024
Filing Date:
July 18, 2023
Export Citation:
Assignee:
NAT CT CHILD HEALTH & DEV (JP)
International Classes:
C12N5/071; C12N5/0735
Foreign References:
| JP2017532047A | 2017-11-02 | |||
| JP2010057513A | 2010-03-18 |
Other References:
TAKAGI RYO, TANUMA-TAKAHASHI AKIKO, AKIYAMA SAEKO, KANEKO WAKANA, MIURA CHIKA, YAMATO MASAYUKI, SHIMIZU TATSUYA, UMEZAWA AKIHIRO: "Laminin-511-derived recombinant fragment and Rho kinase inhibitor Y-27632 facilitate serial cultivation of keratinocytes differentiated from human embryonic stem cells", REGENERATIVE THERAPY, vol. 18, 1 December 2021 (2021-12-01), pages 242 - 252, XP093132653, ISSN: 2352-3204, DOI: 10.1016/j.reth.2021.07.004
Attorney, Agent or Firm:
HARAKENZO WORLD PATENT & TRADEMARK (JP)
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