Title:
METHOD FOR PRODUCING L-CITRULLINE BY USING WHOLE CELL TRANSFORMATION OF RECOMBINANT CORYNEBACTERIUM CRENATUM
Document Type and Number:
WIPO Patent Application WO/2017/071006
Kind Code:
A1
Abstract:
Provided is a Corynebacterium crenatum for recombinant expression of an arginine deiminase, wherein same is a genetically engineered bacterium obtained by means of arginine deiminase genes from Lactobacillus brevis and being connected to an expression vector pXMJ19 and transformed into Corynebacterium crenatum SDNN403. Also provided is a method for catalytically producing L-citrulline by taking whole cells of the engineering bacterium as a biocatalyst and L-arginine as the substrate, and by using the strategy where L-arginine is supplemented in stages.
Inventors:
RAO ZHIMING (CN)
WANG MEIZHOU (CN)
XU MEIJUAN (CN)
ZHANG XIAN (CN)
YANG TAOWEI (CN)
WANG MEIZHOU (CN)
XU MEIJUAN (CN)
ZHANG XIAN (CN)
YANG TAOWEI (CN)
Application Number:
PCT/CN2015/096672
Publication Date:
May 04, 2017
Filing Date:
December 08, 2015
Export Citation:
Assignee:
UNIV JIANGNAN (CN)
International Classes:
C12N1/21; C12N15/77; C12P13/10; C12R1/15
Foreign References:
CN104195162A | 2014-12-10 |
Other References:
ZHANG, FENGQIN ET AL.: "Construction of Genetic Engineering Strain Used in Xylose Metabolic Pathway Study of Cornebacterium Crenatum", JOURNAL OF JINGGANGSHAN UNIVERSITY ( NATURAL SCIENCE EDITION, vol. 35, no. 4, 31 July 2014 (2014-07-31), pages 44 - 47, ISSN: 1674-8085
JIAO, HAITAO ET AL.: "Cloning and Expression of argR Gene from Corynebacterium Crenatum and Arginine Production with the Recombination Strain", CHINA BIOTECHNOLOGY, vol. 31, no. 10, 31 October 2011 (2011-10-31), pages 57 - 62, ISSN: 1671-8135
XIE, HONGCUI ET AL.: "Constitutive Expression of Citrulline Biosynthetic Genes in Corynebacterium Glutamicum", JOURNAL OF NANJING TECH UNIVERSITY ( NATURAL SCIENCE EDITION, vol. 33, no. 4, 31 July 2011 (2011-07-31), pages 43 - 47, ISSN: 1671-7627
JIAO, HAITAO ET AL.: "Cloning and Expression of argR Gene from Corynebacterium Crenatum and Arginine Production with the Recombination Strain", CHINA BIOTECHNOLOGY, vol. 31, no. 10, 31 October 2011 (2011-10-31), pages 57 - 62, ISSN: 1671-8135
XIE, HONGCUI ET AL.: "Constitutive Expression of Citrulline Biosynthetic Genes in Corynebacterium Glutamicum", JOURNAL OF NANJING TECH UNIVERSITY ( NATURAL SCIENCE EDITION, vol. 33, no. 4, 31 July 2011 (2011-07-31), pages 43 - 47, ISSN: 1671-7627
Attorney, Agent or Firm:
HARBIN SHINEIP INTELLECTUAL PROPERTY AGENCY CO., LED (CN)
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