Title:
METHOD FOR PRODUCING LAYERED CELL SHEET AND LAYERED CELL SHEET PRODUCED BY THE SAME
Document Type and Number:
WIPO Patent Application WO/2019/004361
Kind Code:
A1
Abstract:
The present invention provides a method for swiftly producing a layered cell sheet that is non-invasively obtained and is utilizable for transplantation, etc., the method including (1) a step of applying a centrifugal force to a first cell sheet on a temperature-responsive culture surface for a predetermined time in a temperature range from a lower critical solution temperature of the temperature-responsive culture surface to 45℃, (2) a step of further placing a second cell sheet on the first cell sheet, and (3) a step of applying a centrifugal force to the first cell sheet and the second cell sheet on the temperature-responsive culture surface for a predetermined time in the temperature range from the lower critical solution temperature to 45℃; and also provides a layered cell sheet obtained by the method.
Inventors:
KAGAWA YUKI (JP)
SHIMIZU TATSUYA (JP)
HARAGUCHI YUJI (JP)
KUBO HIROTSUGU (JP)
SHIMIZU TATSUYA (JP)
HARAGUCHI YUJI (JP)
KUBO HIROTSUGU (JP)
Application Number:
PCT/JP2018/024605
Publication Date:
January 03, 2019
Filing Date:
June 28, 2018
Export Citation:
Assignee:
NIHON KOHDEN CORP (JP)
UNIV TOKYO WOMENS MEDICAL (JP)
UNIV TOKYO WOMENS MEDICAL (JP)
International Classes:
C12N5/077; A61K35/34; A61L27/38; C12N5/00
Foreign References:
| EP2617810A1 | 2013-07-24 | |||
| JPH02211865A | 1990-08-23 | |||
| JP2017127873A | 2017-07-27 |
Other References:
AKIYUKI HASEGAWA ET AL: "Rapid fabrication system for three-dimensional tissues using cell sheet engineering and centrifugation : RAPID FABRICATION SYSTEM FOR 3D TISSUES", JOURNAL OF BIOMEDICAL MATERIALS RESEARCH. PART A, vol. 103, no. 12, 1 December 2015 (2015-12-01), HOBOKEN, NY, US, pages 3825 - 3833, XP055504173, ISSN: 1549-3296, DOI: 10.1002/jbm.a.35526
YUJI HARAGUCHI ET AL: "Three-Dimensional Human Cardiac Tissue Engineered by Centrifugation of Stacked Cell Sheets and Cross-Sectional Observation of Its Synchronous Beatings by Optical Coherence Tomography", BIOMED RESEARCH INTERNATIONAL, vol. 2017, 1 January 2017 (2017-01-01), pages 1 - 8, XP055504266, ISSN: 2314-6133, DOI: 10.1155/2017/5341702
KUSHIDA A ET AL: "DECREASE IN CULTURE TEMPERATURE RELEASES MONOLAYER ENDOTHELIAL CELL SHEETS TOGETHER WITH DEPOSITED FIBRONECTIN MATRIX FROM TEMPERATURE-RESPONSIVE CULTURE SURFACES", JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, WILEY, NEW YORK, NY, US, vol. 45, no. 4, 15 June 1999 (1999-06-15), pages 355 - 362, XP001204072, ISSN: 0021-9304, DOI: 10.1002/(SICI)1097-4636(19990615)45:4<355::AID-JBM10>3.0.CO;2-7
MASUDA SHINAKO ET AL: "Three-dimensional cardiac tissue fabrication based on cell sheet technology", ADVANCED DRUG DELIVERY REVIEWS, ELSEVIER, AMSTERDAM, NL, vol. 96, 14 May 2015 (2015-05-14), pages 103 - 109, XP029372126, ISSN: 0169-409X, DOI: 10.1016/J.ADDR.2015.05.002
SHIMIZU T ET AL: "Fabrication of pulsatile cardiac tissue grafts using a novel 3-dimensional cell sheet manipulation technique and temperature-responsive cell culture surfaces", CIRCULATION RESEARCH, GRUNE AND STRATTON, BALTIMORE, US, vol. 90, no. 3, 22 February 2002 (2002-02-22), pages e40, XP002285777, ISSN: 0009-7330, DOI: 10.1161/HH0302.105722
HARAGUCHI Y. ET AL.: "Scaffold-free tissue engineering using cell sheet technology", RSC ADV., vol. 2, 2012, pages 2184 - 2190
HASEGAWA A. ET AL.: "Rapid fabrication system for three-dimensional tissues using cell sheet engineering and centrifugation", J. BIOMED. MATER RES. A, vol. 103, 2015, pages 3825 - 3833
HARAGUCHI Y. ET AL.: "Three-dimensional human cardiac tissue engineered by centrifugation of stacked cell sheets and cross-sectional observation of its synchronous beatings by optical coherence tomography", BIOMED. RES. INT., vol. 2017, 2017, pages 5341702
TADAKUMA K. ET AL., BIOMATERIALS, vol. 34, 2013, pages 9018 - 9025
HARAGUCHI Y. ET AL., NAT. PROTOC., vol. 7, 2012, pages 850 - 858
HARAGUCHI Y. ET AL., METHODS MOL. BIOL., vol. 1181, 2014, pages 139 - 155
HASEGAWA; HASEGAWA A. ET AL., J. BIOMED. MATER RES. A., vol. 103, 2015, pages 3825 - 3833
HASEGAWA A. ET AL., J. BIOMED. MATER RES. A., vol. 103, 2015, pages 3825 - 3833
YUJI HARAGUCHI ET AL: "Three-Dimensional Human Cardiac Tissue Engineered by Centrifugation of Stacked Cell Sheets and Cross-Sectional Observation of Its Synchronous Beatings by Optical Coherence Tomography", BIOMED RESEARCH INTERNATIONAL, vol. 2017, 1 January 2017 (2017-01-01), pages 1 - 8, XP055504266, ISSN: 2314-6133, DOI: 10.1155/2017/5341702
KUSHIDA A ET AL: "DECREASE IN CULTURE TEMPERATURE RELEASES MONOLAYER ENDOTHELIAL CELL SHEETS TOGETHER WITH DEPOSITED FIBRONECTIN MATRIX FROM TEMPERATURE-RESPONSIVE CULTURE SURFACES", JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, WILEY, NEW YORK, NY, US, vol. 45, no. 4, 15 June 1999 (1999-06-15), pages 355 - 362, XP001204072, ISSN: 0021-9304, DOI: 10.1002/(SICI)1097-4636(19990615)45:4<355::AID-JBM10>3.0.CO;2-7
MASUDA SHINAKO ET AL: "Three-dimensional cardiac tissue fabrication based on cell sheet technology", ADVANCED DRUG DELIVERY REVIEWS, ELSEVIER, AMSTERDAM, NL, vol. 96, 14 May 2015 (2015-05-14), pages 103 - 109, XP029372126, ISSN: 0169-409X, DOI: 10.1016/J.ADDR.2015.05.002
SHIMIZU T ET AL: "Fabrication of pulsatile cardiac tissue grafts using a novel 3-dimensional cell sheet manipulation technique and temperature-responsive cell culture surfaces", CIRCULATION RESEARCH, GRUNE AND STRATTON, BALTIMORE, US, vol. 90, no. 3, 22 February 2002 (2002-02-22), pages e40, XP002285777, ISSN: 0009-7330, DOI: 10.1161/HH0302.105722
HARAGUCHI Y. ET AL.: "Scaffold-free tissue engineering using cell sheet technology", RSC ADV., vol. 2, 2012, pages 2184 - 2190
HASEGAWA A. ET AL.: "Rapid fabrication system for three-dimensional tissues using cell sheet engineering and centrifugation", J. BIOMED. MATER RES. A, vol. 103, 2015, pages 3825 - 3833
HARAGUCHI Y. ET AL.: "Three-dimensional human cardiac tissue engineered by centrifugation of stacked cell sheets and cross-sectional observation of its synchronous beatings by optical coherence tomography", BIOMED. RES. INT., vol. 2017, 2017, pages 5341702
TADAKUMA K. ET AL., BIOMATERIALS, vol. 34, 2013, pages 9018 - 9025
HARAGUCHI Y. ET AL., NAT. PROTOC., vol. 7, 2012, pages 850 - 858
HARAGUCHI Y. ET AL., METHODS MOL. BIOL., vol. 1181, 2014, pages 139 - 155
HASEGAWA; HASEGAWA A. ET AL., J. BIOMED. MATER RES. A., vol. 103, 2015, pages 3825 - 3833
HASEGAWA A. ET AL., J. BIOMED. MATER RES. A., vol. 103, 2015, pages 3825 - 3833
Attorney, Agent or Firm:
SHIN-EI PATENT FIRM, P.C. (JP)
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