Title:
METHOD FOR PRODUCING LIPID USING THIOESTERASE VARIANT
Document Type and Number:
WIPO Patent Application WO/2014/045793
Kind Code:
A1
Abstract:
A method for producing a lipid, comprising a step of introducing a gene encoding a protein (a) or a protein (b) as mentioned below into a host to produce a transformant and a step of collecting the lipid from the transformant: (a) a protein which has an amino acid sequence lying between position-112 to position-414 in SEQ ID NO: 1 wherein an amino acid residue corresponding to position-209 in SEQ ID NO: 1, i.e., a tryptophan residue, is substituted by a threonine residue, a glutamic acid residue or an alanine residue, and which has a thioesterase activity; and (b) a protein which has an amino acid sequence produced by deleting, substituting, insterting or adding one or several amino acid residues other than the amino acid residue corresponding to position-209 in SEQ ID NO: 1 in the amino acid sequence for the protein (a), and which has a thioesterase activity.
Inventors:
KAWAHARA AKIHITO (JP)
Application Number:
PCT/JP2013/072418
Publication Date:
March 27, 2014
Filing Date:
August 22, 2013
Export Citation:
Assignee:
KAO CORP (JP)
International Classes:
C12N15/09; C12N1/13; C12N1/21; C12N5/10; C12N9/18; C12P7/64
Domestic Patent References:
| WO2011138891A1 | 2011-11-10 | |||
| WO2011077931A1 | 2011-06-30 | |||
| WO1998055633A1 | 1998-12-10 |
Foreign References:
| US20130029387A1 | 2013-01-31 |
Other References:
TAKUTO TOJO ET AL.: "Kokoyashi Yurai Acyl-ACP Thioesterase wa Chusa Shibosan no Seisan ni Kan'yo suru", JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY TAIKAI KOEN YOSHISHU, 5 March 2012 (2012-03-05), pages 2C10A02
FUYUAN IING ET AL.: "Phylogenetic and experimental characterization of an acyl-ACP thioesterase family reveals significant diversity in enzymatic specificity and activity", BMC BIOCHEMISTRY, vol. 12, no. 44, 2011, pages 1 - 16
KIMBERLY M MAYER ET AL.: "Identification of amino acid residues involved in substrate specificity of plant acyl-ACP thioesterases using a bioinformatics-guided approach", BMC PLANT BIOLOGY, vol. 7, no. 1, 2007, pages 1 - 11
JUN LI ET AL.: "Tryptophan Fluorescence of the lux-Specific Vibrio harveyi Acyl-ACP Thioesterase and Its Tryptophan Mutants: Structural Properties and Ligand-Induced Conformational Change", BIOCHEMISTRY, vol. 37, 1998, pages 16130 - 16138
FUYUAN IING ET AL.: "Phylogenetic and experimental characterization of an acyl-ACP thioesterase family reveals significant diversity in enzymatic specificity and activity", BMC BIOCHEMISTRY, vol. 12, no. 44, 2011, pages 1 - 16
KIMBERLY M MAYER ET AL.: "Identification of amino acid residues involved in substrate specificity of plant acyl-ACP thioesterases using a bioinformatics-guided approach", BMC PLANT BIOLOGY, vol. 7, no. 1, 2007, pages 1 - 11
JUN LI ET AL.: "Tryptophan Fluorescence of the lux-Specific Vibrio harveyi Acyl-ACP Thioesterase and Its Tryptophan Mutants: Structural Properties and Ligand-Induced Conformational Change", BIOCHEMISTRY, vol. 37, 1998, pages 16130 - 16138
Attorney, Agent or Firm:
IIDA, Toshizo et al. (JP)
Toshizo Iida (JP)
Toshizo Iida (JP)
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