Title:
METHOD FOR PRODUCING LIPID
Document Type and Number:
WIPO Patent Application WO/2017/183421
Kind Code:
A1
Abstract:
A method for producing a lipid, said method comprising culturing a transformant, in which the expression of a gene encoding one of proteins (A) to (F) is promoted, and thus allowing the transformant to produce a fatty acid or a lipid comprising the same as a constituent. (A) A protein which comprises the amino acid sequence represented by SEQ ID NO:2. (B) A protein which comprises an amino acid sequence having 89% or more sequence identity with the amino acid sequence of the aforesaid protein (A) and has an acyl CoA synthetase activity. (C) A protein which comprises the amino acid sequence represented by SEQ ID NO:4. (D) A protein which comprises an amino acid sequence having 49% or more sequence identity with the amino acid sequence of the aforesaid protein (C) and has an acyl CoA synthetase activity. (E) A protein which comprises the amino acid sequence represented by SEQ ID NO:6. (F) A protein which comprises an amino acid sequence having 85% or more sequence identity with the amino acid sequence of the aforesaid protein (E) and has an acyl CoA synthetase activity.
Inventors:
SUGIHARA SHINJI (JP)
Application Number:
PCT/JP2017/013550
Publication Date:
October 26, 2017
Filing Date:
March 31, 2017
Export Citation:
Assignee:
KAO CORP (JP)
International Classes:
C12N15/09; C12N1/13; C12N9/00; C12N9/16; C12P7/40; C12P7/64
Domestic Patent References:
| WO2015133305A1 | 2015-09-11 | |||
| WO2014103930A1 | 2014-07-03 | |||
| WO2012087982A2 | 2012-06-28 |
Foreign References:
| US20130102040A1 | 2013-04-25 | |||
| JP2004534507A | 2004-11-18 | |||
| JP2008514221A | 2008-05-08 |
Other References:
DATABASE GenBank [online] 1 January 2012 (2012-01-01), "DEFINITION: Nannochloropsis oculata long-chain acyl-coenzyme A synthetase (LACS) mRNA", XP055602127, retrieved from NCBI Database accession no. HQ228564.1
DATABASE UniProtKB [online] 22 February 2012 (2012-02-22), Database accession no. G9BBC7 (G9BBC7_9STRA)
AGNEW, DANIEL E. ET AL.: "Engineering Escherichia coli for production of C12-C14 polyhydroxyalkanoate from glucose", METAB. ENG., vol. 14, no. 6, 2012, pages 705 - 713, XP055108237, DOI: doi:10.1016/j.ymben.2012.08.003
HAGIWARA, HIROSHI: "Development of Sustainable Raw Materials of Fats and Oils using Microalgae", BIO INDUSTRY, vol. 33, 12 July 2016 (2016-07-12), pages 41 - 47
DATABASE UniProtKB [online] 22 February 2012 (2012-02-22), Database accession no. G9BBC7 (G9BBC7_9STRA)
AGNEW, DANIEL E. ET AL.: "Engineering Escherichia coli for production of C12-C14 polyhydroxyalkanoate from glucose", METAB. ENG., vol. 14, no. 6, 2012, pages 705 - 713, XP055108237, DOI: doi:10.1016/j.ymben.2012.08.003
HAGIWARA, HIROSHI: "Development of Sustainable Raw Materials of Fats and Oils using Microalgae", BIO INDUSTRY, vol. 33, 12 July 2016 (2016-07-12), pages 41 - 47
Attorney, Agent or Firm:
IIDA, Toshizo et al. (JP)
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