Title:
METHOD FOR PRODUCING MEDIUM-CHAIN FATTY ACID USING β-KETOACYL-ACP SYNTHASE
Document Type and Number:
WIPO Patent Application WO/2016/021481
Kind Code:
A1
Abstract:
[Problem] To provide a method for producing a medium-chain fatty acid using β-ketoacyl-ACP synthase, or a lipid having said fatty acid as a constituent thereof, and to also provide a gene, a protein, and a transformant used in said method. [Solution] A method for producing a medium-chain fatty acid or a lipid having said fatty acid as a constituent thereof, said method comprising obtaining a transformant by introducing, to a host, a gene that codes a protein (A) or (B), and extracting a medium-chain fatty acid or a lipid having said fatty acid as a constituent thereof from the obtained transformant. A gene, a protein, and a transformant used in said method. (A) A protein comprising an amino acid sequence represented by SEQ ID NO: 1; (B) A protein comprising an amino acid sequence that is at least 90% identical to said amino acid sequence represented by SEQ ID NO: 1, and exhibiting β-ketoacyl-ACP synthase activity specific to a medium-chain acyl-ACP
Inventors:
TOJO TAKUTO (JP)
SUGIHARA SHINJI (JP)
SUGIHARA SHINJI (JP)
Application Number:
PCT/JP2015/071666
Publication Date:
February 11, 2016
Filing Date:
July 30, 2015
Export Citation:
Assignee:
KAO CORP (JP)
International Classes:
C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12P7/64
Foreign References:
| US20030054524A1 | 2003-03-20 | |||
| US20130198904A1 | 2013-08-01 |
Other References:
DEHESH KATAYOON ET AL.: "How can we genetically engineer oilseed crops to produce high levels of medium-chain fatty acids?", EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY, vol. 103, no. 10, 2001, pages 688 - 697
SLABAUGH MARY B. ET AL.: "Condensing enzymes from Cuphea wrightii associated with medium chain fatty acid biosynthesis.", THE PLANT JOURNAL, vol. 13, no. 5, 1998, pages 611 - 620, XP002149886, DOI: doi:10.1046/j.1365-313X.1998.00065.x
DATABASE DDBJ/EMBL/GenBank [o] 31 October 2006 (2006-10-31), CHA T. S. ET AL.: "Definition: Beta-ketoacyl-ACP synthase II", Database accession no. Q9M604
SLABAUGH MARY B. ET AL.: "Condensing enzymes from Cuphea wrightii associated with medium chain fatty acid biosynthesis.", THE PLANT JOURNAL, vol. 13, no. 5, 1998, pages 611 - 620, XP002149886, DOI: doi:10.1046/j.1365-313X.1998.00065.x
DATABASE DDBJ/EMBL/GenBank [o] 31 October 2006 (2006-10-31), CHA T. S. ET AL.: "Definition: Beta-ketoacyl-ACP synthase II", Database accession no. Q9M604
Attorney, Agent or Firm:
IIDA, Toshizo et al. (JP)
Toshizo Iida (JP)
Toshizo Iida (JP)
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