Title:
METHOD FOR PRODUCING MIDDLE-CHAIN Ω-HYDROXY FATTY ACIDS, ΑAND Ω-DICARBOXYLIC ACIDS, AND Ω-AMINO FATTY ACIDS FROM LONG-CHAIN FATTY ACIDS BY BIOTRANSFORMATION
Document Type and Number:
WIPO Patent Application WO/2013/151393
Kind Code:
A1
Abstract:
The present invention relates to a transformant transformed so as to express Baeyer-Villiger monooxygenase (BVMO), a method for producing C5-C14 middle-chain ω-hydroxy fatty acids, αand ω-dicarboxylic acids, ω-amino fatty acids, or alcohols from C16-C20 long-chain fatty acids by biotransformation using the transformant, a method for producing a fatty acid derivative having an ester group introduced into the chain thereof from a keto fatty acid by using the BVMO, and novel ω-hydroxy fatty acids prepared by the method. It is possible to massively generate degradation products such as C5-C14 ω-hydroxy fatty acids, αand ω-dicarboxylic acids, ω-amino fatty acids, alcohols, and the like by degrading C16-C20 long-chain fatty acids contained in a medium through biotransformation by using the transformant capable of expressing BVMO of the present invention, and thus the present invention can be widely used for safer and more economical production of ω-hydroxy fatty acids, αand ω-dicarboxylic acids, ω-amino fatty acids, or alcohols.
Inventors:
PARK JIN BYUNG (KR)
SONG JI WON (KR)
JEON EUN YEONG (KR)
SONG JI WON (KR)
JEON EUN YEONG (KR)
Application Number:
PCT/KR2013/002885
Publication Date:
October 10, 2013
Filing Date:
April 05, 2013
Export Citation:
Assignee:
UNIV EWHA IND COLLABORATION (KR)
International Classes:
C12N15/53; C12N1/21; C12N15/63; C12P7/06
Foreign References:
| US20100285545A1 | 2010-11-11 | |||
| US5952517A | 1999-09-14 | |||
| US6392074B1 | 2002-05-21 | |||
| US5420316A | 1995-05-30 | |||
| US20110105774A1 | 2011-05-05 | |||
| US6392074B1 | 2002-05-21 |
Other References:
KIRSCHNER, A. ET AL.: "Design of a secondary alcohol degradation pathway from Pseudomonas fluorescens DSM 50106 in an engineered Escherichia coli", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 75, no. 5, 9 March 2007 (2007-03-09), pages 1095 - 1101, XP019513723
VAN BEILEN, JAN B. ET AL.: "Cloning of Baeyer-Villiger monooxygenases from Comamonas, Xanthobacter and Rhodococcus using polymerase chain reaction with highly degenerate primers", ENVIRONMENTAL MICROBIOLOGY, vol. 5, no. 3, March 2003 (2003-03-01), pages 174 - 182, XP055169233
HILDEBRANDT, P. ET AL.: "Cloning, functional expression and biochemical characterization of a stereoselective alcohol dehydrogenase from Pseudomonas fluorescens DSM50106", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 59, no. 4-5, 26 June 2002 (2002-06-26), pages 483 - 487, XP055169235
KIRSCHNER, ANETT. ET AL.: "Cloning, expression, and characterization of a Baeyer-Villiger monooxygenase from Pseudomonas fluorescens DSM 50106 in E. coli", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 73, no. 5, 31 August 2006 (2006-08-31), pages 1065 - 1072, XP019472485
BIOTECHNOL. LETT., vol. 29, 2007, pages 1393 - 1398
J. BIOTECHNOL., vol. 158, 2012, pages 17 - 23
APPL. MICROBIOL. BIOTECHNOL., vol. 73, 2007, pages 1065 - 1072
AGRIC BIOL. CHEM., vol. 54, 1990, pages 2039 - 2045
J. BIOTECHNOL., vol. 262, 1987, pages 17712 - 17718
CHEMCATCHEM, vol. 5, 2013, pages 154 - 157
VAN BEILEN, JAN B. ET AL.: "Cloning of Baeyer-Villiger monooxygenases from Comamonas, Xanthobacter and Rhodococcus using polymerase chain reaction with highly degenerate primers", ENVIRONMENTAL MICROBIOLOGY, vol. 5, no. 3, March 2003 (2003-03-01), pages 174 - 182, XP055169233
HILDEBRANDT, P. ET AL.: "Cloning, functional expression and biochemical characterization of a stereoselective alcohol dehydrogenase from Pseudomonas fluorescens DSM50106", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 59, no. 4-5, 26 June 2002 (2002-06-26), pages 483 - 487, XP055169235
KIRSCHNER, ANETT. ET AL.: "Cloning, expression, and characterization of a Baeyer-Villiger monooxygenase from Pseudomonas fluorescens DSM 50106 in E. coli", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, vol. 73, no. 5, 31 August 2006 (2006-08-31), pages 1065 - 1072, XP019472485
BIOTECHNOL. LETT., vol. 29, 2007, pages 1393 - 1398
J. BIOTECHNOL., vol. 158, 2012, pages 17 - 23
APPL. MICROBIOL. BIOTECHNOL., vol. 73, 2007, pages 1065 - 1072
AGRIC BIOL. CHEM., vol. 54, 1990, pages 2039 - 2045
J. BIOTECHNOL., vol. 262, 1987, pages 17712 - 17718
CHEMCATCHEM, vol. 5, 2013, pages 154 - 157
Attorney, Agent or Firm:
SON, MIN (KR)
손민 (KR)
손민 (KR)
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