METHOD FOR PRODUCING MUTANT HUMAN ERYTHROPOIETIN

Title:

METHOD FOR PRODUCING MUTANT HUMAN ERYTHROPOIETIN

Document Type and Number:

WIPO Patent Application WO/2017/154869

Kind Code:

A1

Abstract:

Disclosed is a method for producing mutant human erythropoietin with high purity and high yield. The method is a method for producing mutant human erythropoietin, comprising causing the mutant human erythropoietin to be produced by a transgenic mammalian animal cell and then subjecting a culture supernatant to hydrophobic column chromatography, multimodal anion-exchange column chromatography, anion-exchange column chromatography, phosphate-affinity column chromatography and gel filtration column chromatography sequentially in this order.

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Inventors:

KAKIMOTO SHINJI (JP)
MATEV MIROSLAV (JP)
FUKUI TSUYOSHI (JP)
HATANO YUKICHI (JP)
TANI JUNYA (JP)
MIHARA KAZUTOSHI (JP)
TAKAHASHI KENICHI (JP)
SUGIMURA ATSUSHI (JP)

Application Number:

PCT/JP2017/008907

Publication Date:

September 14, 2017

Filing Date:

March 07, 2017

Export Citation:

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Assignee:

JAPAN CHEM RES (JP)
KISSEI PHARMACEUTICAL (JP)

International Classes:

C12P21/02; C07K1/16; C12N15/09

Domestic Patent References:

WO2013002330A1	2013-01-03
WO2011063195A2	2011-05-26
WO2011024024A1	2011-03-03

Foreign References:

JPH08506023A	1996-07-02
JP2013505276A	2013-02-14
JP2002529475A	2002-09-10
JP2011521993A	2011-07-28

Other References: