Title:
METHOD FOR PRODUCING NAIVE HUMAN IPS CELLS FROM SOMATIC CELLS
Document Type and Number:
WIPO Patent Application WO/2022/138964
Kind Code:
A1
Abstract:
Provided is a method for producing naive induced pluripotent stem cells from human somatic cells, said method comprising the following steps (1) to (3): (1) a step for introducing at least one vector containing an initialization factor into human somatic cells; (2) a step for culturing the somatic cells in the presence of a naive medium; and (3) a step for, after step (2), culturing the obtained cells in the presence of the naive medium under conditions where the amount of the vector per somatic cell is reduced to 30% or less compared to the amount at the start of step 3.
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Inventors:
KUNITOMI AKIRA (JP)
SHU TSUGUMINE (JP)
KAWAGUCHI JITSUTARO (JP)
SHU TSUGUMINE (JP)
KAWAGUCHI JITSUTARO (JP)
Application Number:
PCT/JP2021/048397
Publication Date:
June 30, 2022
Filing Date:
December 24, 2021
Export Citation:
Assignee:
UNIV KYOTO (JP)
ID PHARMA CO LTD (JP)
ID PHARMA CO LTD (JP)
International Classes:
C12N5/10; C12N7/01; C12N15/113; C12N15/12; C12N15/86
Domestic Patent References:
| WO2016148253A1 | 2016-09-22 | |||
| WO2015046229A1 | 2015-04-02 | |||
| WO2012029770A1 | 2012-03-08 | |||
| WO2019093340A1 | 2019-05-16 | |||
| WO2011102444A1 | 2011-08-25 | |||
| WO2020153478A1 | 2020-07-30 | |||
| WO2017082174A1 | 2017-05-18 | |||
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FUSAKI, N.BAN, H.NISHIYAMA, A.SAEKI, K.HASEGAWA, M.: "Efficient induction of transgene-free human pluripotent stem cells using a vector based on Sendai virus, an RNA virus that does not integrate into the host genome", PROCEEDINGS OF THE JAPAN ACADEMY, vol. 85, 2009, pages 348 - 362, XP002663242, DOI: 10.2183/PJAB.85.348
YAN, L. ET AL., NATURE STRUCTURAL & MOLECULAR BIOLOGY, vol. 20, 2013, pages 1131 - 1139
THEUNISSEN T ET AL., CELL STEM CELL, vol. 15, 2014, pages 471 - 487
KILENS S ET AL., NATURE COMMUNICATIONS, vol. 9, 2018, pages 360
LIU X ET AL., NATURE METHODS, vol. 14, 2017, pages 1055
THEUNISSEN, T. W. ET AL.: "Systematic identification of culture conditions for induction and maintenance of naive human pluripotency", CELL STEM CELL, vol. 15, 2014, pages 471 - 487, XP055237395, DOI: 10.1016/j.stem.2014.07.002
YAN, L. ET AL.: "Single-cell RNA-Seq profiling of human preimplantation embryos and embryonic stem cells", NATURE STRUCTURAL & MOLECULAR BIOLOGY, vol. 20, 2013, pages 1131 - 1139, XP055304034, DOI: 10.1038/nsmb.2660
VIRUS, vol. 57, no. 1, 2007, pages 29 - 36
ANNU. REV. GENET., vol. 32, 1998, pages 123 - 162
"Fields virology", 2001, LIPPINCOTT-RAVEN, pages: 1305 - 1340
MICROBIOL. IMMUNOL., vol. 43, 1999, pages 613 - 624
FIELD VIROLOGY, 1996, pages 1205 - 1241
LAMB, R.A.D. KOLAKOFSKY, PARAMYXOVIRIDAE: THE VIRUSES AND THEIR REPLICATION, 1996, pages 1177 - 1204
BLANCHARD L. ET AL., VIROLOGY, vol. 319, 2004, pages 201 - 211
MEDICAL JOURNAL OF OSAKA UNIVERSITY, vol. 6, no. 1, March 1955 (1955-03-01), pages 1 - 15
LI, H.-O. ET AL., J. VIROL., vol. 74, no. 14, 2000, pages 6564 - 6569
J. VIROL., 1995, pages 849 - 855
INOUE, M. ET AL., J. VIROL., vol. 77, 2003, pages 3238 - 3246
HENIKOFF, S.HENIKOFF, J. G., PROC. NATL. ACAD. SCI. USA, vol. 89, 1992, pages 10915 - 10919
MORIKAWA Y. ET AL., KITASATO ARCH. EXP. MED., vol. 64, 1991, pages 15 - 30
"GenBank", Database accession no. AB855655
WRIGHT, K. E. ET AL., VIRUS RES., vol. 67, 2000, pages 49 - 57
J. VIROLOGY, vol. 39, 1981, pages 519 - 528
HASAN, M. K. ET AL., J. GEN. VIROL., vol. 78, 1997, pages 2813 - 2820
KATO, A. ET AL., EMBO J., vol. 16, 1997, pages 578 - 587
YU, D. ET AL., GENES CELLS, vol. 2, 1997, pages 457 - 466
DURBIN, A. P. ET AL., VIROLOGY, vol. 235, 1997, pages 323 - 332
WHELAN, S. P. ET AL., PROC. NATL. ACAD. SCI. USA, vol. 92, 1995, pages 8388 - 8392
SCHNELL. M. J. ET AL., EMBO J., vol. 13, 1994, pages 4195 - 4203
RADECKE, F. ET AL., EMBO J., vol. 14, 1995, pages 6087 - 6094
LAWSON, N. D. ET AL., PROC. NATL. ACAD. SCI. USA, vol. 82, 1985, pages 4477 - 4481
KATO, A. ET AL., GENES CELLS, vol. 1, 1996, pages 569 - 579
BARON, M. D.BARRETT, T., J. VIROL., vol. 71, 1997, pages 1265 - 1271
BRIDGEN, A.ELLIOTT, R. M., PROC. NATL. ACAD.SCI. USA, vol. 93, 1996, pages 15400 - 15404
TOKUSUMI, T. ET AL., VIRUS RES., vol. 86, 2002, pages 33 - 38
"Detailed Experimental Virology (Uirusu-gaku Jikken-gaku Kakuron", 1982, MARUZEN CO. LD.
TAKAHASHI, K.YAMANAKA S., CELL, vol. 126, 2006, pages 663 - 676
LOWRY WE ET AL., PROC NATL ACAD SCI U S A, vol. 105, no. 8, 2008, pages 2883 - 8
MASAKI, H. ET AL., STEM CELL RES., vol. 1, 2008, pages 105 - 115
MOL CELL BIOL., vol. 23, no. 8, 2003, pages 2699 - 708
CELL, vol. 113, no. 63, 2003, pages 1 - 642
NATURE, vol. 423, 2003, pages 541 - 545
DEVELOPMENT, vol. 130, 2003, pages 1673 - 1680
NAT MED, vol. 10, no. 1, 2004, pages 55 - 63
"Techniques in Molecular Biology", 1983, MACMILLAN PUBLISHING COMPANY
KUNKEL ET AL., METHODS ENZYMOL., vol. 154, 1987, pages 367 - 382
SAMBROOK ET AL.: "Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
DAYHOFF ET AL.: "Atlas of Protein Sequence and Structure", 1978, NATL. BIOMED. RES. FOUND.
S. HENIKOFFJ. G. HENIKOFF, PROC. ACAD. NATL. SCI. USA, vol. 89, 1992, pages 10915 - 10919
ALTSCHUL, S. F. BIOL., BIOL., vol. 215, 1990, pages 403 - 410
ALTSCHUL S. F. ET AL., NATURE GENET., vol. 3, 1993, pages 266 - 272
MADDEN, T. L. ET AL., METH. ENZYMOL., vol. 266, 1996, pages 131 - 141
ALTSCHUL S. F. ET AL., NUCLEIC ACIDS RES., vol. 25, 1997, pages 3389 - 3402
ZHANG J.MADDEN T. L., GENOME RES., vol. 7, 1997, pages 649 - 656
TATIANA A ET AL., FEMS MICROBIOL LETT., vol. 174, 1999, pages 247 - 250
CAS, no. 133052-90-1
CAS , no. 109511-58-2
"CAS", Database accession no. 142273-20-9
CAS, no. 1129669-05-1
ALTSCHUL, S. F. ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 410
IRIE T. ET AL., PLOS ONE., vol. 5, 2010, pages e10719
RECHSTEINER M., TRENDS BIOCHEM. SCI., vol. 21, 1996, pages 267 - 271
FUSAKI, N.BAN, H.NISHIYAMA, A.SAEKI, K.HASEGAWA, M.: "Efficient induction of transgene-free human pluripotent stem cells using a vector based on Sendai virus, an RNA virus that does not integrate into the host genome", PROCEEDINGS OF THE JAPAN ACADEMY, vol. 85, 2009, pages 348 - 362, XP002663242, DOI: 10.2183/PJAB.85.348
YAN, L. ET AL., NATURE STRUCTURAL & MOLECULAR BIOLOGY, vol. 20, 2013, pages 1131 - 1139
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YAMAO, Norihito et al. (JP)
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