Title:
METHOD FOR PRODUCING NATURAL KILLER CELLS, NATURAL KILLER CELLS PRODUCED THEREBY, AND COMPOSITION FOR TREATING CANCERS AND INFECTIOUS DISEASES CONTAINING THE SAME
Document Type and Number:
WIPO Patent Application WO/2013/094988
Kind Code:
A1
Abstract:
The present invention relates to a method for producing natural killer cells (hereinafter, referred to as "NK cells"), NK cells produced thereby, and a composition for treating cancers and infectious diseases containing the same. The present invention provides a method for producing NK cells, which maintain high cytotoxicity and cell viability to exhibit high therapeutic effects against cancers and infectious diseases and can be cultured ex vivo at high efficiency and high concentration. In addition, a culture method which maintains cell concentration at a constant level is used for the production of NK cells, and thus the overgrowth of the cells can be prevented so that the cells can be maintained at an optimal state. Particularly, even when the cells are thawed after freezing, the function of the cells is not impaired, and the NK cells can maintain high cell viability and cytotoxicity. Thus, the NK cells can be easily stored and supplied in a liquid or frozen state without needing an additional treatment process.
Inventors:
MIN BOKYUNG (KR)
CHOI HANA (KR)
LIM OKJAE (KR)
HER JUNG HYUN (KR)
KANG SANGMI (KR)
LEE EUN-KYOUNG (KR)
CHUNG HYEJIN (KR)
HWANG YU KYEONG (KR)
CHOI HANA (KR)
LIM OKJAE (KR)
HER JUNG HYUN (KR)
KANG SANGMI (KR)
LEE EUN-KYOUNG (KR)
CHUNG HYEJIN (KR)
HWANG YU KYEONG (KR)
Application Number:
PCT/KR2012/011114
Publication Date:
June 27, 2013
Filing Date:
December 18, 2012
Export Citation:
Assignee:
MOGAM BIOTECH RES INST (KR)
GREEN CROSS LAB CELL CORP (KR)
GREEN CROSS LAB CELL CORP (KR)
International Classes:
C12N5/0783; A61K35/17; A61K35/56; A61P35/00; C12N5/02
Domestic Patent References:
| WO2010013947A2 | 2010-02-04 | |||
| WO2009151183A1 | 2009-12-17 | |||
| WO2008138214A1 | 2008-11-20 | |||
| WO2008023874A1 | 2008-02-28 |
Foreign References:
| KR101133185B1 | 2012-04-06 | |||
| KR20110132618A | 2011-12-08 | |||
| KR101035556B1 | 2011-05-23 | |||
| KR20080008060A | 2008-01-23 | |||
| US20030068306A1 | 2003-04-10 |
Other References:
LI, XIAO-HONG ET AL.: "Ex vivo expansion of highly purified NK cells from human peripheral blood", ZHONGGUO SHIYAN XUEYEXUE ZAZHI - JOURNAL OF EXPERIMENTAL HEMATOLOGY, ZHONGGUO SHIYAN XUEYEXUE ZAZHISHE, BEIJING, CN, vol. 15, no. 2, 1 April 2007 (2007-04-01), pages 373 - 377, XP009160095
See also references of EP 2794859A4
See also references of EP 2794859A4
Attorney, Agent or Firm:
LEE, Cheo Young et al. (Yeosam Bldg.648-23, Yeoksam-dong,Gangnam-gu, Seoul 135-080, KR)
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Claims: