Title:
METHOD FOR PRODUCING NUCLEIC ACID MOLECULE
Document Type and Number:
WIPO Patent Application WO/2024/048684
Kind Code:
A1
Abstract:
The present disclosure provides a method for producing a nucleic acid molecule. In detail, the present disclosure realizes process shortening and dramatic efficiency in ligation by using nucleic acid splints. Specifically, the present disclosure is a method for producing a polynucleotide molecule having a specific sequence, the method including 1) a step of producing two or more polynucleotide fragment molecules including a part of said specific sequence and 2) a step for connecting said two or more polynucleotide fragment molecules in the presence of two or more nucleic acid splints. Said specific sequence is formed when all the sequences of said two or more polynucleotide fragment molecules are assembled. Said nucleic acid splits are substantially complementary to said polynucleotide molecules. When at least two of said nucleic acid splints align with said specific sequence, a sequence in which there is substantially no deletion is obtained.
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Inventors:
SANOSAKA MASATO (JP)
SAITO EMI (JP)
NANKAI HIROKAZU (JP)
SAITO EMI (JP)
NANKAI HIROKAZU (JP)
Application Number:
PCT/JP2023/031631
Publication Date:
March 07, 2024
Filing Date:
August 30, 2023
Export Citation:
Assignee:
GENEDESIGN INC (JP)
International Classes:
C12N15/10; C12N15/11
Domestic Patent References:
| WO2021108647A1 | 2021-06-03 |
Foreign References:
| JP2016098198A | 2016-05-30 |
Other References:
ZHOU LIJUN, O’FLAHERTY DEREK K., SZOSTAK JACK W.: "Assembly of a Ribozyme Ligase from Short Oligomers by Nonenzymatic Ligation", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, vol. 142, no. 37, 16 September 2020 (2020-09-16), pages 15961 - 15965, XP093145545, ISSN: 0002-7863, DOI: 10.1021/jacs.0c06722
LIJUN ZHOU; DEREK K. O'FLAHERTY; JACK W. SZOSTAK: "Template‐Directed Copying of RNA by Non‐enzymatic Ligation", ANGEWANDTE CHEMIE INTERNATIONAL EDITION, VERLAG CHEMIE, HOBOKEN, USA, vol. 59, no. 36, 13 August 2020 (2020-08-13), Hoboken, USA, pages 15682 - 15687, XP072091490, ISSN: 1433-7851, DOI: 10.1002/anie.202004934
LIJUN ZHOU; DEREK K. O'FLAHERTY; JACK W. SZOSTAK: "Template‐Directed Copying of RNA by Non‐enzymatic Ligation", ANGEWANDTE CHEMIE INTERNATIONAL EDITION, VERLAG CHEMIE, HOBOKEN, USA, vol. 59, no. 36, 13 August 2020 (2020-08-13), Hoboken, USA, pages 15682 - 15687, XP072091490, ISSN: 1433-7851, DOI: 10.1002/anie.202004934
Attorney, Agent or Firm:
KAMADA, Mitsunori et al. (JP)
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