Title:
METHOD FOR PRODUCING OLIGOSACCHARIDE HAVING LEWIS X SKELETON
Document Type and Number:
WIPO Patent Application WO/2023/153461
Kind Code:
A1
Abstract:
The present invention relates to a microorganism having an enhanced activity of any one of the following proteins :[1] a protein composed of an amino acid sequence represented by SEQ ID NO: 2; [2] a mutant protein that is composed of an amino acid sequence in which 1-20 amino acids in the amino acid sequence represented by SEQ ID NO: 2 have been deleted, substituted, inserted or added and that has an α1,3-fucosyltransferase activity; [3] a homologous protein that is composed of an amino acid sequence having 90% or greater identity with the amino acid sequence represented by SEQ ID NO: 2 and that has an α1,3-fucosyltransferase activity; and [4] a protein composed of an amino acid sequence represented by SEQ ID NO: 24, 18 or 26. This microorganism also has improved productivity of an oligosaccharide having a Lewis X skeleton compared with the parent strain.
Inventors:
SUGITA TOMOTOSHI (JP)
Application Number:
PCT/JP2023/004262
Publication Date:
August 17, 2023
Filing Date:
February 08, 2023
Export Citation:
Assignee:
KIRIN HOLDINGS KK (JP)
International Classes:
C12N1/21; C12N15/54; C12P19/18
Domestic Patent References:
| WO2022168991A1 | 2022-08-11 | |||
| WO2022168992A1 | 2022-08-11 |
Foreign References:
| JP2019115349A | 2019-07-18 |
Other References:
DATABASE UniprotKB 24 June 2015 (2015-06-24), ANONYMOUS : "Alpha-(1,3)-fucosyltransferase FucT N-terminal domain-containing protein", XP093083858, retrieved from Uniprot Database accession no. A0A0F5J8L9
DATABASE UniprotKB 22 May 2017 (2017-05-22), ANONYMOUS : "Glycosyltransferase family 10 (Fucosyltransferase) C-term", XP093083860, retrieved from Uniprot Database accession no. A0A1I3AU36
SUGITA TOMOTOSHI; SAMPEI SOTARO; KOKETSU KENTO: "Efficient production of lacto-N-fucopentaose III in engineered Escherichia coli using α1,3-fucosyltransferase from Parabacteroides goldsteinii", JOURNAL OF BIOTECHNOLOGY, ELSEVIER, AMSTERDAM NL, vol. 361, 9 December 2022 (2022-12-09), Amsterdam NL , pages 110 - 118, XP087235161, ISSN: 0168-1656, DOI: 10.1016/j.jbiotec.2022.12.002
DATABASE UniprotKB 22 May 2017 (2017-05-22), ANONYMOUS : "Glycosyltransferase family 10 (Fucosyltransferase) C-term", XP093083860, retrieved from Uniprot Database accession no. A0A1I3AU36
SUGITA TOMOTOSHI; SAMPEI SOTARO; KOKETSU KENTO: "Efficient production of lacto-N-fucopentaose III in engineered Escherichia coli using α1,3-fucosyltransferase from Parabacteroides goldsteinii", JOURNAL OF BIOTECHNOLOGY, ELSEVIER, AMSTERDAM NL, vol. 361, 9 December 2022 (2022-12-09), Amsterdam NL , pages 110 - 118, XP087235161, ISSN: 0168-1656, DOI: 10.1016/j.jbiotec.2022.12.002
Attorney, Agent or Firm:
Eikoh, P.C. (JP)
Download PDF:
Previous Patent: POWER CIRCUIT AND METHOD FOR SUPPLYING POWER SUPPLY VOLTAGE
Next Patent: VEHICULAR LAMP
Next Patent: VEHICULAR LAMP