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Title:
METHOD FOR PRODUCING ORGANIC ACID
Document Type and Number:
WIPO Patent Application WO/2016/163382
Kind Code:
A1
Abstract:
Provided is a method for producing an organic acid, whereby it becomes possible to produce the organic acid from a microalga effectively. An organic acid can be produced effectively by allowing carbon dioxide and an optical energy to be utilized directly by a microalga and then collecting the organic acid, which is an intercellular metabolite of the microalga, from the microalga. Specifically, an organic acid is produced by culturing a microalga in an aqueous medium containing carbonate ions and/or bicarbonate ions and then collecting the organic acid which is an intercellular metabolite of the microalga. Alternatively, an organic acid is produced by culturing a microalga, in which the function of a NADPH-O2 oxidoreductase and/or the function of a rate-controlling enzyme in the glycolytic system from glycogen to the citric acid cycle is enhanced, in an aqueous medium and then collecting the organic acid which is an intercellular metabolite of the microalga. For example, succinic acid, among organic acids, has been produced utilizing petroleum or the like as a raw material. According to the method of the present invention, an organic acid can be produced by effectively utilizing a biomass. The organic acid produced by the method can be used effectively in foods, medicines and other various fields.

Inventors:
HASUNUMA, Tomohisa (1-1 Rokkodai-cho, Nada-ku, Kobe-sh, Hyogo 01, 〒6578501, JP)
Application Number:
JP2016/061216
Publication Date:
October 13, 2016
Filing Date:
April 06, 2016
Export Citation:
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Assignee:
NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY (1-1 Rokkodai-cho, Nada-ku Kobe-sh, Hyogo 01, 〒6578501, JP)
International Classes:
C12P7/40; C12N1/13; C12P7/44; C12P7/46; C12N1/21; C12N15/09
Domestic Patent References:
2012-03-29
Foreign References:
JP2015073469A2015-04-20
JP2010507369A2010-03-11
Other References:
HASUNUMA T. ET AL.: "Overexpression of flv3 improves photosynthesis in the cyanobacterium Synechocystis sp. PCC6803 by enhancement of alternative electron flow", BIOTECHNOLOGY FOR BIOFUELS, vol. 7, no. 493, 31 December 2014 (2014-12-31), pages 1 - 10, XP021210424
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CHI Z. ET AL.: "Bicarbonate-based integrated carbon capture and algae production system with alkalihalophilic cyanobacterium", BIORESOURCE TECHNOLOGY, vol. 133, 9 February 2013 (2013-02-09), pages 513 - 521, XP055319974
CHI Z. ET AL.: "Bicarbonate produced from carbon capture for algae culture", TRENDS IN BIOTECHNOLOGY, vol. 29, no. 11, November 2011 (2011-11-01), pages 537 - 541, XP028318598
OSANAI T. ET AL.: "Genetic manipulation of a metabolic enzyme and a transcriptional regulator increasing succinate excretion from unicellular cyanobacterium", FRONTIERS IN MICROBIOLOGY, vol. 6, 6 October 2015 (2015-10-06), pages 1 - 10, XP055319980
ANGERMAYR S. A. ET AL.: "Exploring metabolic engineering design principles for the photosynthetic production of lactic acid by Synechocystis sp. PCC6803", BIOTECHNOLOGY FOR BIOFUELS, vol. 7, no. 99, June 2014 (2014-06-01), pages 1 - 15, XP021191102
Attorney, Agent or Firm:
SHOJI, Takashi et al. (#307 Miyuki Bldg, 5-6-13 Nishinakajima, Yodogawa-ku, Osaka-sh, Osaka 11, 〒5320011, JP)
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